We narrowed to 23,191 results for: his
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Plasmid#226401PurposeExpresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged 20D3Q mutant 2N4R tau for recombinant protein production in bacteriaDepositorAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only
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2BT-His-TEV-cs-LC3b
Plasmid#197460PurposeBacterial Expression of human LC3bDepositorAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
Rab11A[8-175]-His-C Q70L
Plasmid#228042PurposeProtein purification construct. Rab11 crystallography truncation with a C-terminal cys for maleimide labeling. Constitutively active mutation (Q70L).DepositorAvailable SinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L_Δ1,2-linker_pcDNA3.1(+)
Plasmid#187727PurposeExpress his-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in mammalian cellsDepositorInsertNEDD4L with residues 226-382 removed (NEDD4L Human)
TagsHis-HRV 3C siteExpressionMammalianMutationdeletion of residues 226-382Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L_Δ2,3-linker_pcDNA3.1(+)
Plasmid#187728PurposeExpress his-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in mammalian cellsDepositorInsertNEDD4L with residues 418-495 removed (NEDD4L Human)
TagsHis-HRV 3C siteExpressionMammalianMutationdeletion of residues 418-495Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-a(+)-6xHis-miRFP(-5)-HO1
Plasmid#219424PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertsmiRFP682
HO1
TagsMGHHHHHGGExpressionBacterialMutationContains Ribosomal Binding Site for expression of…PromoterT7Available SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21a HsDHFR-bio-10His
Plasmid#193883PurposeExpresses human dihydrofolate reductase (DHFR) with C-terminal AviTag (Bio) and His10 sequenceDepositorInserthuman dihydrofolate reductase (DHFR) (DHFR Human)
TagsHis tag and biotinylation sequenceExpressionBacterialMutationCodon optimizedPromoterT7Available SinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPPI4-Strep-SNAP-PD-L2-His10
Plasmid#223578PurposeProtein purification of human PD-L2 with SNAP and 10xHis tags in mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCM2(413-438)_pHisSUMO(K0)
Plasmid#224065PurposeBacterial expression of the C-terminal helix of CCM2 (residues 413-438) fused to an N-terminal hexa-histidine tag and SUMO(K0) [all lysines in SUMO mutated to arginines]. HRV-3C cleavage siteDepositorAvailable SinceAug. 26, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)
Plasmid#222990PurposeBacterial expression of iGeoCas9(C1) constructDepositorInsertHis-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)
UseCRISPRTagsCL7, His, and MBPExpressionBacterialPromotertacAvailable SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX304-HsTtyh1-TwinStrep-His
Plasmid#211332PurposeMammalian overexpression vector for C-terminally TwinStrep and His tagged human Ttyh1 (W417R)DepositorInsertTweety homology protein 1
UseLentiviralTagsTwinStrep, 10xHisExpressionMammalianMutationW417RPromoterCMVAvailable SinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-HsProm1s1-TwinStrep-His
Plasmid#210816PurposeMammalian overexpression vector for C-terminally TwinStrep and His tagged human Prom1s1 (WT)DepositorAvailable SinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-hIsl2-EGFP
Plasmid#153206PurposeHuman Isl2 (hIsl2) promoter-mediates gene expression in retinal neurons with fluorescent reporterDepositorTypeEmpty backboneUseAAVExpressionMammalianAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-ccdB-SNAP
Plasmid#166146PurposeGateway destination vector for generation of N-terminal His10-tagged and C-terminal SNAP-tagged proteins in E. coli.DepositorTypeEmpty backboneTagsHis10 and SNAPExpressionBacterialAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-ccdB
Plasmid#166147PurposeGateway destination vector for generation of N-terminal His10-Smt3 tagged proteins in E. coli.DepositorTypeEmpty backboneTagsHis10-Smt3ExpressionBacterialAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-DDX5(kf)-SNAP
Plasmid#166152PurposeExpression of N. furzeri DDX5 in E. coliDepositorInsertDDX5
TagsHis10 and SNAPExpressionBacterialPromoterT7Available SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC-HIS6-SUMO1-AVITAG-GG
Plasmid#215808PurposeCloning of target gene with N terminal His6 SUMO2 tag and SENP1 cleavage non cleavable N-terminal avitagDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBac1-His6-E3(1)-CUbo-TwinStrep
Plasmid#212712PurposeExpression of His6-E3(1)-CUbo-TwinStrep in insect cellsDepositorInsertE3(1)
Tags6xHis and CUbo-TwinStrepExpressionBacterialAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H2_mCherry(-6)-L1
Plasmid#205050PurposeCo-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H2_mCherry(-6)-L1
TagsGRRGKKSRK and MGHHHHHGGAExpressionBacterialAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H2_mCherry(-6)-L2
Plasmid#205051PurposeCo-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H2_mCherry(-6)-L2
TagsGRRGKKSRKGRRGKKSRK and MGHHHHHGGAExpressionBacterialAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H2_mCherry(-6)-L3
Plasmid#205052PurposeCo-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H2_mCherry(-6)-L3
TagsGRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGAExpressionBacterialAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo(K48R)-His6
Plasmid#212778PurposeC-terminal PCR-based tagging with CUbo(K48R)-His6 in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingAvailable SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo(K63R)-His6
Plasmid#212779PurposeC-terminal PCR-based tagging with CUbo(K63R)-His6 in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingAvailable SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo-yeGFP-His6
Plasmid#212782PurposeC-terminal PCR-based tagging with CUbo-yeGFP-His6 in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingAvailable SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-Duet-C17orf53-6His
Plasmid#211417Purposebacterial expression of human C17orf53 C-terminally fused to 6xHis-TagDepositorAvailable SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-Duet-6His-C17orf53
Plasmid#211416Purposebacterial expression of human C17orf53 N-terminally fused to a 6xHis-TagDepositorAvailable SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUra3-pCUP1-His7-NUbo
Plasmid#212761PurposeN-terminal PCR-based tagging with pCUP1-His7-NUbo in budding yeast with URA3 selectionDepositorTypeEmpty backboneUsePcr-based taggingAvailable SinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His6-CUbo-MCS
Plasmid#212725PurposeBacterial expression of a protein of interest with N-terminal His6-CUbo-fusion, contains multiple cloning siteDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His7-NUbo-MCS
Plasmid#212724PurposeBacterial expression of a protein of interest with N-terminal His7-NUbo-fusion, contains multiple cloning siteDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-NUbo-MCS-His6
Plasmid#212723PurposeBacterial expression of a protein of interest with N-terminal NUbo- and C-terminal His6-fusions, contains multiple cloning siteDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His6-CUbo-GFP
Plasmid#212720PurposeBacterial expression of His6-CUbo-GFPDepositorInsertGFP
Tags6xHis-CUboExpressionBacterialAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His7-NUbo-GFP
Plasmid#212719PurposeBacterial expression of His7-NUbo-GFPDepositorInsertGFP
Tags7xHis-NUboExpressionBacterialAvailable SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-NUbo-GFP-His6
Plasmid#212718PurposeBacterial expression of NUbo-GFP-His6DepositorInsertGFP
Tags6xHis and NUboExpressionBacterialAvailable SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30-His6-TEV-Mms2
Plasmid#212716PurposeBacterial expression of His6-Mms2, His6-tag cleavable with TEV-proteaseDepositorInsertMms2
Tags6xHis-TEVExpressionBacterialAvailable SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-TEV2-Ubc7-His6
Plasmid#212713PurposeBacterial expression of MBP-Ubc7-His6, MBP-tag cleavable with TEV-proteaseDepositorInsertUbc7
Tags6xHis and MBP-TEV2ExpressionBacterialAvailable SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_StammerAAA
Plasmid#202587PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purificationDepositorInsertORF1p
Tags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Methionine 91 to Alanine, ORF1p Glu…Available SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_StammerAEA
Plasmid#202588PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purificationDepositorInsertORF1p
Tags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Methionine 91 to Alanine, changed O…Available SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-HypaSpCas9-NLS-6xHis
Plasmid#207384PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertHypaSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationN692A, M694A, Q695A, H698APromoterT7Available SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TEV-A7 (2-180)
Plasmid#190066PurposeCodon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain.DepositorInsertAnnexin A7 PRD (ANXA7 Human)
TagsGB1-His-TEVExpressionBacterialMutationPRD only, residues 2-180Available SinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-mRhubarb713-HO1
Plasmid#197254PurposeExpresses the protein of mRhubarb713 and HO1 in bacteriaDepositorInsertmRhubarb713
ExpressionBacterialAvailable SinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only