We narrowed to 6,616 results for: rel
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Plasmid#202597PurposeExpress N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cellDepositorTypeEmpty backboneTagsTEV cleavable site (ENLYFQG) and poly-histidine a…ExpressionMammalianPromoterCAGAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only
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pCAGGS-N-TEV-HAT
Plasmid#202598PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cellDepositorTypeEmpty backboneTagsTEV cleavable site (ENLYFQG) and poly-histidine a…ExpressionMammalianPromoterCAGAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOET3-C-TEV-Twin-Strep
Plasmid#203324PurposeGenerate the baculovirus to express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cellsDepositorTypeEmpty backboneTagsTEV cleavable site (ENLYFQG) and Twin-strep-tagExpressionInsectAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GST-RAPH1-F2
Plasmid#194864PurposeExpress the RAPH1 fragment (535-868) tagged to GST in bacteria.DepositorInsertRAPH1 1062-1250 (RAPH1 Human)
TagsGSTExpressionBacterialMutationamino acids 1062-1250PromoterTacAvailable SinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
eGFP-RAPH1-F4
Plasmid#194863PurposeExpress the RAPH1 fragment (1062-1250) tagged to eGFP in mammalian cells.DepositorInsertRAPH1 (RAPH1 Human)
TagseGFPExpressionMammalianMutationamino acids 1062-1250PromoterCMVAvailable SinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
eGFP-RAPH1-F3
Plasmid#194862PurposeExpress the RAPH1 fragment (868-1062) tagged to eGFP in mammalian cells.DepositorInsertRAPH1 (RAPH1 Human)
TagseGFPExpressionMammalianMutationamino acids 868-1062PromoterCMVAvailable SinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
eGFP-RAPH1-F2
Plasmid#194861PurposeExpress the RAPH1 fragment (535-868) tagged to eGFP in mammalian cells.DepositorAvailable SinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-M2-Cterm_H
Plasmid#146482PurposeInsect Expression of DmGW182-M2-CtermDepositorInsertDmGW182-M2-Cterm (gw Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmeIF4E-siRNAres-V5His6_H
Plasmid#146487PurposeInsect Expression of DmeIF4E-siRNAresDepositorInsertDmeIF4E-siRNAres
ExpressionInsectMutation3 silent mutations C411A, T531C, C652T compared t…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGW182-M2-Cterm_H
Plasmid#146495PurposeInsect Expression of DmGW182-M2-CtermDepositorInsertDmGW182-M2-Cterm (gw Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmPop2a-V5His6_G
Plasmid#146390PurposeInsect Expression of DmPop2aDepositorInsertDmPop2a (Pop2 Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGW182-delDUFdelCterm_G
Plasmid#146393PurposeInsect Expression of DmGW182-delDUFdelCtermDepositorInsertDmGW182-delDUFdelCterm (gw Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGW182-delMGWdelRBD_G
Plasmid#146394PurposeInsect Expression of DmGW182-MGWdelRBDDepositorInsertDmGW182-MGWdelRBD (gw Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGW182-delQdelMGW_G
Plasmid#146395PurposeInsect Expression of DmGW182-delQdelMGWDepositorInsertDmGW182-delQdelMGW (gw Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
GLRX-roGFP2 in pT3TS-Dest
Plasmid#194297PurposeIn vitro transcription of the redox sensor GLRX-roGFP2 from the T3 promoter. Parton lab clone KRKDepositorInsertGLRX-roGFP2
UseIn vitro transcription of mrnaAvailable SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcrVIB1-cloDF-amp
Plasmid#184843PurposeExpression of anti-CRISPR protein AcrVIB1 in bacteria.DepositorInsertanti-CRISPR protein inhibiting Cas13b nucleases
UseSynthetic BiologyExpressionBacterialPromoterJ23119Available SinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBSKΔB-24xopto-TetO-no01
Plasmid#174887PurposePlasmid vector containing southern blot probe for opto-TetO in STREAMING-tagDepositorInsert24xopto-TetO
UseSouthern blot probeAvailable SinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME-p3E-GLRX-roGFP2
Plasmid#194288PurposeMultisite gateway entry clone for expression of the redox probe GLRX-roGFP2. Parton lab clone KQFDepositorInsertGLRX-roGFP2
UseMultisite gateway entry cloneAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAcrVIB1-cloDF-kan
Plasmid#184836PurposeExpression of anti-CRISPR protein AcrVIB1 in bacteria.DepositorInsertanti-CRISPR protein inhibiting Cas13b nucleases
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-PGK-SNAP-MDMUT-OCT4
Plasmid#115692PurposeConstitutive expression of an OCT4 (Pou5f1) protein tagged with SNAP-tag and a non-functional mitotic degron (MDMUT) for controlDepositorInsertSNAP-MDMUT-OCT4
UseLentiviralAvailable SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGW182-delUBAdelQrich_G
Plasmid#146396PurposeInsect Expression of DmGW182-delUBAdelQrichDepositorInsertDmGW182-delUBAdelQrich (gw Fly)
ExpressionInsectAvailable SinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-Drp1(K569A,K571A)-StrepII
Plasmid#174426PurposeExpresses human Drp1 Isoform 3 with K569A, K571A mutations in bacteriaDepositorInsertDrp1
Tags6xHis(TEV) and StrepIIExpressionBacterialMutationK569A, K571APromoterT7Available SinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRD445
Plasmid#168775PurposeExpression of mEos3.2 in MycobacteriumDepositorInsertmEos3.2
ExpressionBacterialAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-Drp1(K557A,K560A)-StrepII
Plasmid#174425PurposeExpresses human Drp1 Isoform 3 with K557A, K560A mutations in bacteriaDepositorInsertDrp1
Tags6xHis(TEV) and StrepIIExpressionBacterialMutationK557A, K560APromoterT7Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-Flag-NEDD4L∆WW4
Plasmid#166710Purposeexpresses NEDD4L with inactivating mutation in fourth WW domain in mammalian cellsDepositorInsertNEDD4L W576A
TagsFLAGExpressionMammalianMutationchanged tryptophan 576 to alaninePromoterCMV promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PP-NEDD4L-WW1(193-226)
Plasmid#166701Purposeexpresses NEDD4L WW1 in bacterial cellsDepositorInsertNEDD4L WW1 domain
TagsGST, HRV 3C site, and Thrombin siteExpressionBacterialPromotertac promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PP-NEDD4L-WW2(385-418)
Plasmid#166702Purposeexpresses NEDD4L WW2 in bacterial cellsDepositorInsertNEDD4L WW2 domain
TagsGST, HRV 3C site, and Thrombin siteExpressionBacterialPromotertac promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PP-NEDD4L-WW3(497-530)
Plasmid#166703Purposeexpresses NEDD4L WW3 in bacterial cellsDepositorInsertNEDD4L WW3 domain
TagsGST, HRV 3C site, and Thrombin siteExpressionBacterialPromotertac promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PP-NEDD4L-WW4(548-581)
Plasmid#166704Purposeexpresses NEDD4L WW4 in bacterial cellsDepositorInsertNEDD4L WW4 domain
TagsGST, HRV 3C site, and Thrombin siteExpressionBacterialPromotertac promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-Flag-NEDD4L∆WW1
Plasmid#166707Purposeexpresses NEDD4L with inactivating mutation in first WW domain in mammalian cellsDepositorInsertNEDD4L W221A
TagsFLAGExpressionMammalianMutationchanged tryptophan 221 to alaninePromoterCMV promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBA cMYC-BPC4
Plasmid#118668PurposePlant overexpressionDepositorAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_vhhGFP4::Nrv1::tagBFP
Plasmid#163925PurposeExpression of GrabFP-B-extracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (extracellular) nanobody with Nrv1 protein and tagBFP fluorophor
UseCre/LoxTagstagBFPExpressionInsectAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10AC1)_Psyn-sgRNArodA
Plasmid#149659Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRExpressionBacterialPromoterPflaB, PpQE30(-10AC1), PsynAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10AC2)_Psyn-sgRNArodA
Plasmid#149660Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRExpressionBacterialPromoterPflaB, PpQE30(-10AC2), PsynAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_Psyn-sgRNAmreB
Plasmid#149595Purposeall-in-one CRISPRi vector for targeting B. burgdorferi mreBDepositorInsertdCas9, lacI, sgRNAmreB
ExpressionBacterialPromoterPflaB, PpQE30(-10TC), PsynAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_Psyn-sgRNAftsI
Plasmid#149591Purposeall-in-all CRISPRi vector for targeting B. burgdorferi ftsIDepositorInsertdCas9, lacI, sgRNAftsI
UseCRISPRExpressionBacterialPromoterPflaB, PpQE30(-10TC), PsynAvailable SinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-RNAPII-A2-14-HA
Plasmid#160689PurposeExpression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains an S-to-A mutation in position 2 of 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.DepositorInsertRNA polymerase II CTD
ExpressionBacterialMutationContains an S-to-A mutation in position 2 of 14 o…Available SinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_Psyn-sgRNArodA
Plasmid#149657Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRExpressionBacterialPromoterPflaB, PpQE30(-10TC), PsynAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_Psyn-sgRNAflaB
Plasmid#149589Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
ExpressionBacterialPromoterPflaB, PpQE30(-10TC), PsynAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15-His-∆NGlpG(Y205F)
Plasmid#155132PurposeExpresses N-terminally His-tagged E. coli GlpG Y205F variant (residues 87-276) from pET15bDepositorInsertGlpG (glpG Escherichia coli)
TagsHisExpressionBacterialMutationY205F, insert codes for GlpG residues 87-276PromoterT7Available SinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS-ATG1-ATG7 3'UTR(406)
Plasmid#122070PurposeExpressing ATG1 with ATG7 3'UTRDepositorInsertserine/threonine protein kinase ATG1 (ATG1 Budding Yeast)
ExpressionYeastMutationThe native 3'UTR was replaced by ATG7 3'…Available SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluSnFR E25R
Plasmid#119831PurposeProduction of recombinant iGluSnFR E25R variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluSnFR E25R
TagsGSTExpressionBacterialMutationE25RPromoterT7Available SinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
TIAM2_PDZ_1
Plasmid#103952PurposeProtein expression and purification of TIAM2 PDZ domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
SNTA1_PDZ_1
Plasmid#103928PurposeProtein expression and purification of STNA1 PDZ domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MPP1_PDZ_1
Plasmid#103931PurposeProtein expression and purification of MPP1 PDZ domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
INADL_PDZ_5
Plasmid#103905PurposeProtein expression and purification of INADL PDZ5 domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
INADL_PDZ_6
Plasmid#103906PurposeProtein expression and purification of INADL PDZ6 domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
INADL_PDZ_7
Plasmid#103907PurposeProtein expression and purification of INADL PDZ7 domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MPP6_PDZ_1
Plasmid#103924PurposeProtein expression and purification of P55 PDZ domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
SCRIB_PDZ_2
Plasmid#103884PurposeProtein expression and purification of Scribble PDZ2 domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only