We narrowed to 22,137 results for: his

1. pET28a-anticalin (N116amb)-His

   Plasmid

   #168045

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Use

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Asn 116 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a-anticalin (N21amb)-His

   Plasmid

   #168041

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Use

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Asn 21 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET15b p97-His D592Amb

   Plasmid

   #169022

   Purpose

   Expresses p97-His D592Amb (incorporates noncanonical amino acid at position 592 via amber suppression) in bacteria

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   p97
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   D592TAG

   Promoter

   T7

   Available Since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a-anticalin (I55amb)-His

   Plasmid

   #168042

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Use

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Ile 55 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAc5.1B-lambdaN-DmPABPC1-V5His6_D

   Plasmid

   #146095

   Purpose

   Insect Expression of DmPABPC1

   Depositor

   Elisa Izaurralde

   Insert

   DmPABPC1 (pAbp Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   seven silent mutations compared to the sequence g…

   Promoter

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET19b-HisDAD2 cPGFP(G164)

   Plasmid

   #165997

   Purpose

   Expression of coding sequence in Escherichia coli

   Depositor

   Claudia Vickers

   Article

   Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

   Insert

   HisDAD2 cPGFP(G164)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDB.His.MBP.3C CARD8 CARD A452C

   Plasmid

   #164023

   Purpose

   Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (A452C mutation for cysteine labelling applications)

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Mutation

   A452C

   Promoter

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDB.His.MBP.3C CARD8 UPA-STOP

   Plasmid

   #164031

   Purpose

   Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 UPA

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDB.His.MBP.3C NLRP1 CARD G1467C

   Plasmid

   #164009

   Purpose

   Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 CARD G1467C

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Mutation

   G1467C

   Promoter

   Available Since

   Feb. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pQE80L-Coh-His-SpyTag

    Plasmid

    #157673

    Purpose

    Expression of Coh (type III from Ruminococcus flavefaciens) with His6x tag and SpyTag.

    Depositor

    Michael Nash

    Article

    Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

    Insert

    Cohesin (R.f) with Histag and SpyTag
    

    Use

    Tags

    Histag and SpyTag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTricHis-ILY-HB2lock-N342W

    Plasmid

    #160536

    Purpose

    Expression of inserted ILY variant

    Depositor

    Doryen Bubeck

    Article

    Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

    Insert

    ILY-HB2lock-N342W
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Y208C, D322C, N342W

    Promoter

    Available Since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTricHis-ILY-HB2lock-L340W

    Plasmid

    #160535

    Purpose

    Expression of inserted ILY variant

    Depositor

    Doryen Bubeck

    Article

    Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

    Insert

    ILY-HB2lock-L340W
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Y208C, D322C, L340W

    Promoter

    Available Since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTricHis-ILY-HB2lock-N338W

    Plasmid

    #160534

    Purpose

    Expression of inserted ILY variant

    Depositor

    Doryen Bubeck

    Article

    Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

    Insert

    ILY-HB2lock-N338W
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Y208C, D322C, N338W

    Promoter

    Available Since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTricHis-ILY-HB2lock-Y336W

    Plasmid

    #160533

    Purpose

    Expression of inserted ILY variant

    Depositor

    Doryen Bubeck

    Article

    Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

    Insert

    ILY-HB2lock-Y336W
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Y208C, D322C, Y336W

    Promoter

    Available Since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTricHis-ILY-HB2lock-no charge

    Plasmid

    #160532

    Purpose

    Expression of inserted ILY variant

    Depositor

    Doryen Bubeck

    Article

    Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

    Insert

    ILY-HB2lock-no charge
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Y208C, D322C, E337A, N338A, K341A, N342A

    Promoter

    Available Since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTricHis-ILY-HB2lock-charge+

    Plasmid

    #160531

    Purpose

    Expression of inserted ILY variant

    Depositor

    Doryen Bubeck

    Article

    Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

    Insert

    ILY-HB2lock-charge+
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Y208C, D322C, Y336K, L340K

    Promoter

    Available Since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTricHis-ILY-HB1lock-N342W

    Plasmid

    #160529

    Purpose

    Expression of inserted ILY variant

    Depositor

    Doryen Bubeck

    Article

    Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

    Insert

    ILY-HB1lock-N342W
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    A221C, N404C, N342W

    Promoter

    Available Since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTricHis-ILY-HB1lock-L340W

    Plasmid

    #160528

    Purpose

    Expression of inserted ILY variant

    Depositor

    Doryen Bubeck

    Article

    Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

    Insert

    ILY-HB1lock-L340W
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    A221C, N404C, L340W

    Promoter

    Available Since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTricHis-ILY-HB1lock-N338W

    Plasmid

    #160527

    Purpose

    Expression of inserted ILY variant

    Depositor

    Doryen Bubeck

    Article

    Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

    Insert

    ILY-HB1lock-N338W
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    A221C, N404C, N338W

    Promoter

    Available Since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTricHis-ILY-HB1lock-Y336W

    Plasmid

    #160526

    Purpose

    Expression of inserted ILY variant

    Depositor

    Doryen Bubeck

    Article

    Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

    Insert

    ILY-HB1lock-Y336W
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    A221C, N404C, Y336W

    Promoter

    Available Since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTricHis-ILY-HB1lock-no charge

    Plasmid

    #160525

    Purpose

    Expression of inserted ILY variant

    Depositor

    Doryen Bubeck

    Article

    Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

    Insert

    ILY-HB1lock-no charge
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    A221C, N404C, E337A, N338A, K341A, N342A

    Promoter

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTricHis-ILY-HB1lock-charge+

    Plasmid

    #160524

    Purpose

    Expression of inserted ILY variant

    Depositor

    Doryen Bubeck

    Article

    Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

    Insert

    ILY-HB1lock-charge+
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    A221C, N404C, Y336K, L340K

    Promoter

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTricHis-ILY-prepore-charge+

    Plasmid

    #160522

    Purpose

    Expression of inserted ILY variant

    Depositor

    Doryen Bubeck

    Article

    Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

    Insert

    ILY-prepore-charge+
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    I104C, G244C, Y336K, L340K

    Promoter

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTricHis-ILY-prepore-N342C

    Plasmid

    #160521

    Purpose

    Expression of inserted ILY variant

    Depositor

    Doryen Bubeck

    Article

    Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

    Insert

    ILY-prepore-N342C
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    I104C, G244C, N342C

    Promoter

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTricHis-ILY-prepore-L340C

    Plasmid

    #160520

    Purpose

    Expression of inserted ILY variant

    Depositor

    Doryen Bubeck

    Article

    Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

    Insert

    ILY-prepore-L340C
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    I104C, G244C, L340C

    Promoter

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA-HisFlag-rUSP19ER-CAmut

    Plasmid

    #155237

    Purpose

    for mammalian overexpression of the mutated ER isoform of rat USP19

    Depositor

    Simon Wing

    Article

    Lu et al Mol Cell Biol. 2009 Jan;29(2):547-58. do

    Insert

    USP19 (ER isoform) (Usp19 Rat)
    

    Use

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Mutation

    Cys(545) to Ala

    Promoter

    CMV

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA-HisFlag-rUSP19ER-CRmut

    Plasmid

    #155253

    Purpose

    for mammalian overexpression of the mutated ER isoform of rat USP19

    Depositor

    Simon Wing

    Insert

    USP19 (ER isoform) CR mutant (Usp19 Rat)
    

    Use

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Mutation

    Cys(545) to Arg

    Promoter

    CMV

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA-HisFlag-rUSP19ER deltaN3

    Plasmid

    #155243

    Purpose

    for mammalian overexpression of truncated (delta1-1631) ER isoform of rat USP19

    Depositor

    Simon Wing

    Insert

    USP19 (delta1-1631) (ER isoform) (Usp19 Rat)
    

    Use

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA-HisFlag-rUSP19ER deltaN2

    Plasmid

    #155242

    Purpose

    for mammalian overexpression of truncated (delta1-1088) ER isoform of rat USP19

    Depositor

    Simon Wing

    Insert

    USP19 (delta1-1088) (ER isoform) (Usp19 Rat)
    

    Use

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA-HisFlag-rUSP19ER deltaN1

    Plasmid

    #155241

    Purpose

    for mammalian overexpression of truncated (delta1-536) ER isoform of rat USP19

    Depositor

    Simon Wing

    Insert

    USP19 (delta1-536) (ER isoform) (Usp19 Rat)
    

    Use

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET15-His-∆NGlpG(Y205F)

    Plasmid

    #155132

    Purpose

    Expresses N-terminally His-tagged E. coli GlpG Y205F variant (residues 87-276) from pET15b

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Y205F, insert codes for GlpG residues 87-276

    Promoter

    T7

    Available Since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHybr_r2a>Fab-srt-his

    Plasmid

    #124810

    Purpose

    HDR template to knock-in sortag and histag in rat IgG2a heavy chain locus. Use in combination with PX459-gR2A_Hinge to convert rat IgG2a hybridoma to Fab' fragment producing cell lines.

    Depositor

    Ferenc Scheeren, Martijn Verdoes

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    rat IgG2a Hinge-G4S-Sortag-Histag
    

    Use

    CRISPR; Hdr template

    Tags

    Histag (HHHHHH) and Sortag (LPETGG)

    Expression

    Bacterial

    Mutation

    Promoter

    pUC

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1/NDUFS2 _2-33/myc-His

    Plasmid

    #127492

    Purpose

    Expresses myc-tagged _MTS NDUFS2 in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 _2-33 (NDUFS2 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 _2-33

    Promoter

    CMV

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1/NDUFS2 _2-78/myc-His

    Plasmid

    #127493

    Purpose

    Expresses myc-tagged _2-78 NDUFS2 in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 _2-78 (NDUFS2 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 _2-78

    Promoter

    CMV

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAS1-rab-3P::his-24::mOrange

    Plasmid

    #124346

    Purpose

    Nuclear Localized Pan-Neuronal mOrange

    Depositor

    Andrew Leifer

    Insert

    rab-3, mOrange (rab-3 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Smp_141500-Cd4-bio-His

    Plasmid

    #120699

    Purpose

    Expression of recombinant S. mansoni protein

    Depositor

    Gavin Wright

    Article

    Cécile et al bioRXiv

    Insert

    Smp_141500 (Smp_141500.1 Schistosoma mansoni)
    

    Use

    Tags

    ratCd4(d3+4)-bio-6His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Smp_006060-Cd4-bio-His

    Plasmid

    #120700

    Purpose

    Expression of recombinant S. mansoni protein

    Depositor

    Gavin Wright

    Article

    Cécile et al bioRXiv

    Insert

    Smp_006060 (Smp_006060 Schistosoma mansoni)
    

    Use

    Tags

    ratCd4(d3+4)-bio-6His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Smp_063330-Cd4-bio-His

    Plasmid

    #120701

    Purpose

    Expression of recombinant S. mansoni protein

    Depositor

    Gavin Wright

    Article

    Cécile et al bioRXiv

    Insert

    Smp_063330 (Smp_063330 Schistosoma mansoni)
    

    Use

    Tags

    ratCd4(d3+4)-bio-6His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Smp_096790-Cd4-bio-His

    Plasmid

    #120702

    Purpose

    Expression of recombinant S. mansoni protein

    Depositor

    Gavin Wright

    Article

    Cécile et al bioRXiv

    Insert

    Smp_096790 (Smp_096790 Schistosoma mansoni)
    

    Use

    Tags

    ratCd4(d3+4)-bio-6His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Smp_201730-Cd4-bio-His

    Plasmid

    #120703

    Purpose

    Expression of recombinant S. mansoni protein

    Depositor

    Gavin Wright

    Article

    Cécile et al bioRXiv

    Insert

    Smp_201730 (Smp_201730 Schistosoma mansoni)
    

    Use

    Tags

    ratCd4(d3+4)-bio-6His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge