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Plasmid#186717PurposeACME dual-deaminase CRISPR base editorDepositorInsertACME
UseCRISPRTagsNucleoplasmin NLSExpressionMammalianMutationSee manuscriptPromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKI1.1
Plasmid#85807PurposeCRISPR/Cas9 for Arabidopsis in pENTR/D-TOPODepositorInserthuman-codon-optimized SpCas9
UseCRISPRTagsFLAG and NLSExpressionMutationPromoterAtRPS5AAvailable sinceMay 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMZ288
Plasmid#59897PurposeCombination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6+DepositorInsertsCas9
rrk1:sgRNA
UseCRISPRTagsExpressionYeastMutationSilent muation of the CspCI sitePromoterAvailable sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMZ289
Plasmid#59898PurposeCombination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-M210DepositorInsertsCas9
rrk1:sgRNA
UseCRISPRTagsExpressionYeastMutationSilent muation of the CspCI sitePromoterAvailable sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMZ381
Plasmid#59899PurposeCombination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-L469DepositorInsertsCas9
rrk1:sgRNA
UseCRISPRTagsExpressionYeastMutationSilent muation of the CspCI sitePromoterAvailable sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEL082
Plasmid#137891PurposeiSTU-CRISPR/Cas9 with OsCDPK2intron, RZ structure backboneDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-CasRx-non-targeting (pEZ18)
Plasmid#229049PurposeCloning backbone for CasRx crRNA (U6 promoter)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6-Cas13bt1-non-targeting (pJK2)
Plasmid#229054PurposeCloning backbone for Cas13bt1 crRNA (U6 promoter)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
phU1-Cas13bt1-non-targeting (pEZ25)
Plasmid#229055PurposeCloning backbone for Cas13bt1 crRNA (Human U1 promoter)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6-RanCas13b-non-targeting (pJK6)
Plasmid#229056PurposeCloning backbone for RanCas13b crRNA (U6 promoter)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
phU1-RanCas13b-non-targeting (pEZ45)
Plasmid#229057PurposeCloning backbone for RanCas13b crRNA (Human U1 promoter)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Homology Arms-BRD4-HaloTag
Plasmid#228575PurposeCRISPR donor plasmid to tag human BRD4 with Halo tag; for CRISPR-mediated gene insertionDepositorInsertHomology Arms-BRD4-HaloTag (BRD4 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSM-dCas9::Ap
Plasmid#154307PurposeShuttle vector for expression dCas9, mobilization cluster, expresses the tracrRNA and a CRISPR array designed for the easy cloning of new spacers.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
JME4599
Plasmid#129660PurposeNATex_CrisprCas9-yl_RFP: CAS9 vector with Nourseothricin ex marker for gRNA cloning using GoldenGateDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
px330-mcherry
Plasmid#98750PurposeCas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiSAMPHv2
Plasmid#167934PurposeAll-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRGEB31
Plasmid#51295PurposeBinary vector derived from pRGE31. Deliver sgRNA and Cas9 into plants by agrobacterium mediated transformation.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterRice snoRNA U3 and dual 35S promoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSEVA451-Cpf1
Plasmid#192362PurposeCRISPR-based vector for genome editingDepositorInsertCas12a, gRNA scaffold
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSGKP-km
Plasmid#117233PurposeA sgRNA expression plasmid for genome editing in Klebsiella PneumoniaeDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPUR-hU6-sgRNA-Sirius-8XMS2
Plasmid#121942PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-8XMS2
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only