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pTRANS_213

Plasmid

#91112

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: WDV

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pDL52 (clone76)

Plasmid

#188579

Purpose

High-yield production of coenzyme F420 in E. coli

Depositor

Gerald Lackner

Publication

Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

Inserts

ribA
ribD
yigB
fbiC
cofC
cofD
cofI
cofE

Expression

Bacterial

Mutation

Codon optimization, synthetic ribosome binding si…

Promoter

T7 variants

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pTRANS_233

Plasmid

#91122

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: WDV

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pFP.R275

Plasmid

#138224

Purpose

Expresses the split mCherry reporter interrupted by the PhoRadA intein (in cis)

Depositor

Baojun Wang

Publication

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(SGK).PhoRadA.(TQL)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBad_crtEBI_27

Plasmid

#63806

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Publication

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pJPC12-ΔM13-mCherry-P/PM,4A5T6T-GFP

Plasmid

#80913

Purpose

Reporter plasmid (mCherry and GFP)

Depositor

Mark Isalan

Publication

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

mCherry
GFP

Expression

Bacterial

Promoter

P4A5T6T and PM,4A5T6T

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pWT050a

Plasmid

#107899

Purpose

W2.4-2 plasmid. Contains PLacO-sgRNA1, PRha-sgRNA4 and is part of CAMERA 2.4

Depositor

David Liu

Publication

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PLacO-sgRNA1, PRha-sgRNA4

Expression

Bacterial

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-3xGFP-tDeg

Plasmid

#191371

Purpose

Overexpression of 3xGFP-tDeg in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

3xGFP-tDeg

Tags

3xGFP

Expression

Mammalian

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-Halo-tDeg

Plasmid

#191370

Purpose

Overexpression of Halo-tDeg in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Halo-tDeg

Tags

Halo

Expression

Mammalian

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pJ204-loxP-100RPU.2-coEPO-lox2272

Plasmid

#154828

Purpose

Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of erythropoietin

Depositor

Lise Marie Grav

Publication

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Insert

coEPO

Use

Cre/Lox

Expression

Mammalian

Mutation

Codon-optimized (Cricetulus griseus)

Promoter

100RPU.2

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV2-N587X

Plasmid

#130877

Purpose

Encodes the AAV2 capsid with the N587 residue replaces with a TAG stop codon

Depositor

Prashant Mali

Publication

Katrekar et al Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.

Insert

AAV2 cap N587X

Use

AAV

Expression

Mammalian

Mutation

N587X

Promoter

Unknown

Available since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pTK750

Plasmid

#114718

Purpose

Express mCherry-NuMA(5A-3) from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Publication

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NuMA 5A-3

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAN-AND

Plasmid

#62308

Purpose

synthetic circuit: AND gate

Depositor

Christopher Voigt

Publication

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Inserts

A1NT sgRNA
A4NT sgRNA
A2NT sgRNA
A1NT

Use

CRISPR

Expression

Bacterial

Promoter

PA2, PPhlF, pA4, and pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-NLS-ddMisCas13b-SNAPf-NLS-3xFlag

Plasmid

#191537

Purpose

Overexpression of NLS-ddMisCas13b-SNAPf-NLS-3xFlag in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NLS-ddMisCas13b-SNAPf-NLS-3xFlag

Expression

Mammalian

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDIRECT_26H

Plasmid

#91150

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:bar

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers

Use

CRISPR

Expression

Plant

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pJ204-loxP-EF1a-tagBFP-HygR-lox2272-BGHpA-siteT9-HDR

Plasmid

#154827

Purpose

Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site T9)

Depositor

Lise Marie Grav

Publication

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

TagBFP
HygR
ZsGreen1-DR

Use

CRISPR and Cre/Lox

Tags

PEST

Expression

Mammalian

Promoter

CMV, EF1a, and SV40 early promoter

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p426*-LbSgH

Plasmid

#136992

Purpose

Helper plasmid for LbCpf1 gRNA cloning and expression

Depositor

Huimin Zhao

Publication

Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

Type

Empty backbone

Expression

Yeast

Promoter

SNR52

Available since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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p426*-SaSgH

Plasmid

#136994

Purpose

Helper plasmid for SaCas9 gRNA cloning and expression

Depositor

Huimin Zhao

Publication

Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

Type

Empty backbone

Expression

Yeast

Promoter

SNR52

Available since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAN-PTet-YFP

Plasmid

#62245

Purpose

used to characterize the promoter activities of PTet for dCas9 and sgRNA response functions.

Depositor

Christopher Voigt

Publication

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

YFP

Expression

Bacterial

Promoter

Ptet

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pET30a-SARS-CoV2-NP

Plasmid

#165090

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Publication

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)

Expression

Bacterial

Available since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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