We narrowed to 823 results for: Anti-CRISPR

1. pLenti-NIrD

   Plasmid

   #65010

   Purpose

   NS3/4A Inducible rtTA-mediated Dual-reporter system for Hepatitis C Virus (HCV) infection

   Depositor

   Wensheng Wei

   Article

   Ren et al Sci Rep. 2015 Mar 9;5:8865. doi: 10.1038

   Inserts

   rtTA-MAVS(C)
   2A-linked delta-TK
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Tight-TRE

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

2. LLP789_αGCN4-DNMT3A-CO

   Plasmid

   #211783

   Purpose

   SunTag counterpart binding domain, aGCN4, fused to DNA methyltransferase DNMT3A, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   aGCN4-DNMT3A
   

   Use

   Tags

   3xTy1

   Expression

   Mammalian

   Mutation

   Last 300 bp codon optimised (CO) to detect DNMT3A…

   Promoter

   pEF1a and pSV40

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCryptDel4.8

   Plasmid

   #141293

   Purpose

   Plasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.

   Depositor

   Neel Joshi

   Article

   Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

   Inserts

   RelB
   gRNA targetting pMUT2
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Native promoter from pMUT2 relB/relE operon and P…

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB_TRE3G_scFV-sfGFP-G9aCD

   Plasmid

   #235580

   Purpose

   Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector G9a fused with scFV to programme H3K9me2/3

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   G9a (Ehmt2 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB_TRE3G_scFV-sfGFP-Ezh2FL

   Plasmid

   #235575

   Purpose

   Dox-inducible expression of epigenetic effector Ezh2 fused with scFV to programme H3K27me3

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   Ezh2 (Ezh2 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB_TRE3G_scFV-sfGFP-Dot1LCD

   Plasmid

   #235582

   Purpose

   Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Dot1l fused with scFV to programme H3K79me2

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   Dot1l (Dot1l Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. scFv-GCN4-DNMT3a-DNMT3l

   Plasmid

   #154140

   Purpose

   Expresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag systems.

   Depositor

   Albert Jeltsch

   Article

   Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

   Insert

   scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
   

   Use

   Tags

   HA and sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB_TRE3G_scFV-sfGFP-Dot1LCD-CatMut

   Plasmid

   #235591

   Purpose

   Dox-inducible expression of control catalytic-mutant Dot1l CD fused with scFV

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   Dot1l (Dot1l Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   GS163-164RC

   Promoter

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. Plas-gRNA-LEU2

   Plasmid

   #157656

   Purpose

   GFP and gRNA tag for protein and mRNA quantification, to attache to the 3' end of the gene coding sequence

   Depositor

   Frank Albert

   Article

   Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

   Insert

   GFP-gRNA
   

   Use

   Insert storage (replicative in e. coli)

   Tags

   GFP-gRNA

   Expression

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-KrasG12D/sgKras/Cre

    Plasmid

    #99851

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Promoter

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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