We narrowed to 12,327 results for: pcdna

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His-Precission-NEDD4L_C942S_pCDNA3.1(+)

Plasmid

#187729

Purpose

Express the catalytically inactive NEDD4L FL in mammalian cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L FL with C874 mutated to a serine (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

Cys874 mutated to a serine

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Neo(+) hSUMF1

Plasmid

#24386

Depositor

Carolyn Bertozzi

Article

Wu et al Proc Natl Acad Sci U S A. 2009 Mar 3. 10

Insert

hSUMF1 (SUMF1 Human)

Expression

Mammalian

Available Since

April 2, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO (pEHA1339)

Plasmid

#209088

Purpose

Backbone plasmid for Tet Responsive transgene expression, FRT integration

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1.Gαs-LgB113

Plasmid

#134362

Purpose

Nanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.

Depositor

Julien Hanson

Article

Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

Insert

Gαs-LgB113 (GNAS Human)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

T7

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_nucNanobody IRES EGFP

Plasmid

#136619

Purpose

Plasmid encoding nuclear localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

SNAP-Tag-EnhancerNb-NLS (IRES EGFP)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pcdna3 flag - ULK2 wt

Plasmid

#27637

Depositor

Reuben Shaw

Article

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse ULK2 (Ulk2 Mouse)

Tags

flag

Expression

Mammalian

Mutation

Initial M removed from ULK2.

Available Since

March 16, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1+/mit-2mutAEQ

Plasmid

#45539

Depositor

Javier Alvarez-Martin

Article

de la Fuente et al Biochem J. 2012 Aug 1;445(3):371-6. doi:

Insert

mit-2mutAEQ

Tags

Mitochondrially targeted

Expression

Mammalian

Mutation

Asp119Ala and Asn28Leu

Available Since

July 3, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HDAC2-H141A

Plasmid

#115346

Purpose

Expresses mouse HDAC2 cDNA with H141A point mutation

Depositor

Javier Gonzalez-Maeso

Article

Kurita et al Nat Neurosci. 2012 Sep;15(9):1245-54. do

Insert

Histone deacetylase 2 (Hdac2 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

H141A

Promoter

CMV

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mCherry-CyrCR1

Plasmid

#128768

Purpose

Expresses mCherry in mammalian cells. ~200 nt of Cyrano inserted in 3'UTR with miR-7 site replaced by miR-17 site.

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

Cyrano conserved region (CR1) with intact miR-7 site (OIP5-AS1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Ikkb-FLAG WT

Plasmid

#23298

Depositor

Warner Greene

Article

Geleziunas et al Mol Cell Biol. 1998 Sep . 18(9):5157-65.

Insert

Ikkbeta (IKBKB Human)

Tags

Flag

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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TLS 5:hTLS-CHOP.pCDNA1

Plasmid

#21831

Depositor

David Ron

Article

Crozat et al Nature. 1993 Jun 17;363(6430):640-4.

Insert

TLS-CHOP (FUS Human)

Expression

Mammalian

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Rheb

Plasmid

#19996

Depositor

Fuyuhiko Tamanoi

Article

Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

Ras homolog enriched in brain 1 (RHEB Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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hKCNQ3(WT).ires.mCherry-pcDNA3

Plasmid

#204362

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

KCNQ3 (KCNQ3 Human)

Expression

Mammalian

Promoter

CMV/T7 RNApol

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 M

Plasmid

#158078

Purpose

Expresses SARS-CoV-2 membrane protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 matrix (M )

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-mito-ApplePy1Highest

Plasmid

#239074

Purpose

Mammalian expression of mitochondria-targeted highest affinity red fluorescent pyruvate biosensor ApplePy1Highest

Depositor

Robert Campbell

Article

Imai et al bioRxiv 2025.04.17.649293

Insert

ApplePy1Highest

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf6(WT)-mCherry

Plasmid

#79422

Purpose

Expresses C-terminally mCherry-tagged Arf6(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf6 (ARF6 Human)

Tags

mCherry

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK6 K231R

Plasmid

#80884

Purpose

mammalian expression of ALK6 K231R

Depositor

Aristidis Moustakas

Article

Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

Insert

ALK6 (Bmpr1b Mouse)

Tags

HA

Expression

Mammalian

Mutation

K231R (kinase-deficient)

Promoter

CMV

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag HBx

Plasmid

#42596

Depositor

Ding Xue

Article

Geng et al Proc Natl Acad Sci U S A. 2012 Nov 6;109

Insert

Hepatitis B virus protein X

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Flag-Timeless

Plasmid

#22887

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2005 Apr . 25(8):3109-16.

Insert

Timeless (TIMELESS Human)

Tags

Flag

Expression

Mammalian

Mutation

deleted normal start codon, replaced with start c…

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-Sintbad

Plasmid

#234668

Purpose

expresses SINTBAD (TBKBP1) in mammalian cells

Depositor

Lienhard Schmitz

Article

Saul et al iScience. 2019 Sep 27;19:527-544. doi: 1

Insert

SINTBAD (TBKBP1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf1(WT)-mCherry

Plasmid

#79419

Purpose

Expresses C-terminally mCherry-tagged Arf1(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf1 (ARF1 Human)

Tags

mCherry

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag C/EBPa

Plasmid

#66978

Purpose

Expression plasmid for mouse C/EBPa; adds Flag tag to the N-terminus of protein

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

C/EBP alpha (Cebpa Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-neo-Strep_Flag_Cterm

Plasmid

#102645

Purpose

Epitope tag c-terminus with SBP-Flag moiety

Depositor

Michael E. Wright

Article

Hsiao et al BMC Cancer. 2015 Mar 31;15:204. doi: 10.

Type

Empty backbone

Tags

SBP-Flag, MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPG…

Expression

Mammalian

Available Since

Oct. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3β-FLAG-CBP-LD-HA

Plasmid

#32906

Depositor

Tso-Pang Yao

Article

Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

Insert

C/EBP (Crebbp Mouse)

Tags

FLAG and HA

Expression

Mammalian

Mutation

L1435A/D1436A

Promoter

CMV

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 E

Plasmid

#158080

Purpose

Expresses SARS-CoV-2 envelope protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 envelope (E )

Expression

Mammalian

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-30nmEML-mRFP

Plasmid

#231179

Purpose

Stabilizes the ER-mitochondrial distance at 30 nm; tagged with mRFP

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

30nmEML-mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RIPK1

Plasmid

#78842

Purpose

To overexpress RIPK1 in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK1 (RIPK1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ 2xFLAG-2xSTREP_KEAP1

Plasmid

#159128

Purpose

expresses KEAP1 in mammalian cells

Depositor

Michele Pagano

Article

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Insert

KEAP1 (KEAP1 Human)

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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2MLS-HyperNova/pcDNA3

Plasmid

#221457

Purpose

Expressing HyperNova in mitochondria

Depositor

Kiwamu Takemoto

Article

Shidara et al Commun Biol. 2024 Aug 6;7(1):945. doi: 1

Insert

HyperNova fused with a tandem repeat of the mitochondria localization signal from cytochrome c oxidase subunit VIII

Expression

Mammalian

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DN-hCUL4A-FLAG

Plasmid

#15821

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005;399:287-309. doi:

Insert

Cullin4A (CUL4A Human)

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N440

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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