We narrowed to 7,808 results for: 199

1. 6CCR (GID4)

   Plasmid

   #119960

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   GID4 (C17orf39 Human)
   

   Use

   Tags

   6xHIS, thrombin,SUMO and TEV cleavage

   Expression

   Bacterial

   Mutation

   contains amino acids 116-300

   Promoter

   T7

   Available since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pAAV-CAG-BDFP1.6

   Plasmid

   #197199

   Purpose

   Expresses the protein of BDFP1.6 in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   BDFP1.6
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PCDHGC5-EGFP

   Plasmid

   #119933

   Purpose

   Protocadherin gamma C5 expression (EGFP-tag at the C-terminus).

   Depositor

   Angel de Blas

   Article

   Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

   Insert

   PCDHGC5 (Pcdhgc5 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. apt-CBE-3'end

   Plasmid

   #219939

   Purpose

   Mammalian expression of apt-CBE-3'end protein (MCP_32aa-linker_evoAPOBEC1) with P2A-EGFP marker

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   MCP_32-aa_evo-rA1_2xUGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. apt-ABE8e

   Plasmid

   #219937

   Purpose

   Mammalian expression of apt-ABE8e protein (Com_93aa-linker_TadA8e) with P2A-EGFP marker

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   Com_93-aa_TadA8e
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAd5-B1-deltaE1-MCS

   Plasmid

   #122558

   Purpose

   Modified block 1, with deletion in E1

   Depositor

   Fred Bunz

   Article

   Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

   Insert

   Adenovirus 5 genomic region 1-3759
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Adenovirus sequences 481-3530 replaced with multi…

   Promoter

   Available since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Nano-lantern(ATP1)/pcDNA3

   Plasmid

   #51992

   Purpose

   mammalian expression of a Nano-lantern-based luminescent ATP indicator

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_ATP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

8. 6CDG (GID4)

   Plasmid

   #119961

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   GID4 (C17orf39 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   contains amino acids 124-289

   Promoter

   T7

   Available since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pGFP Glra2 (CC#301)

   Plasmid

   #15199

   Depositor

   Connie Cepko

   Article

   Young et al Neuron. 2004 Mar 25. 41(6):867-79.

   Insert

   Glra2 (GLRA2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. nCas9-NG_P2A-mCherry

    Plasmid

    #219934

    Purpose

    Mammalian expression of Sp-nCas9-NG with P2A-mCherry marker for use with MOBE systems

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    Sp-nCas9(D10A)-NG_P2A-mCherry
    

    Use

    CRISPR

    Tags

    SV40 bpNLS and SV40 bpNLS-P2A-mCherry

    Expression

    Mammalian

    Mutation

    nCas9(D10A)

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPM2212

    Plasmid

    #167552

    Purpose

    Encodes rfb region of Shigella flexneri serotype 2a, which expresses Shigella O antigen in E. coli K-12

    Depositor

    Renato Morona

    Article

    Macpherson et al Mol Microbiol. 1991 Jun;5(6):1491-9. doi

    Insert

    rfb region of Shigella flexneri serotype 2a
    

    Use

    Cosmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-HA-MAVS-M142A(KaganF61)

    Plasmid

    #51999

    Purpose

    Expresses the human MAVS CDS containing the point mutation M142A and an N-terminal HA tag "strong translational context"

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Met 142 to Ala

    Promoter

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pTRANS_101

    Plasmid

    #91199

    Purpose

    Transformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: BeYDV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMaCTag-12

    Plasmid

    #119991

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mScarlet
    

    Use

    Pcr template

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRK5_tAKARα-T391A

    Plasmid

    #119920

    Purpose

    Mammalian expression of the tAKARα sensor with T391A mutation

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKARα-T391A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCXN2-mVIN

    Plasmid

    #11776

    Depositor

    Eileen Adamson

    Article

    Xu et al J Cell Sci. 1998 Jun;111 ( Pt 11):1535-4

    Insert

    vinculin (Vcl Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pMaCTag-15

    Plasmid

    #119994

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with miRFP670

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    miRFP670
    

    Use

    Pcr template

    Tags

    miRFP670

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMK409 (mAID-Nluc BSD)

    Plasmid

    #121199

    Purpose

    C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    mAID-Nluc BSD
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMaCTag-11

    Plasmid

    #119990

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet-i

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mScarlet-i
    

    Use

    Pcr template

    Tags

    mScarlet-i

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMYs-IRES-mitoRFP

    Plasmid

    #121996

    Purpose

    Expression of mitoRFP in mammalian cells

    Depositor

    Qing Deng

    Article

    Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

    Insert

    IRES-mitoRFP
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge