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Plasmid#234883Purposenon-targeting CRISPRi controlDepositorInsertL1HS gRNA
UseCRISPR and LentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
shRNA_RNF170_1
Plasmid#215206PurposeSupression of shRNF170_1 expressionDepositorInsertRNF170 shRNA 1 (RNF170 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
EHMT1_exon 3_gRNA
Plasmid#228809PurposeCRISPR targeting of human EHMT1DepositorInsertgRNA targeting EHMT1 exon 3 (EHMT1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0_shRNAHsCavin1_EGFP
Plasmid#229688PurposeLentiviral expression of shRNA targeting Cavin1DepositorInsertshRNA for hCavin1 (CAVIN1 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterLTR viral promoterAvailable sinceJan. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-TadA8e-Sauri-U6-Camk2d sgRNA1
Plasmid#220117PurposeExpresses SauriABE8e by EFS promoter and sgRNA targeting murine Camk2d start codon site by U6 promoterDepositorInsertTadA8e, nSauriCas9
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MYL2-TadA8e-Sauri-U6-Camk2d sgRNA1
Plasmid#220120PurposeExpresses SauriABE8e by MYL2 promoter and sgRNA targeting murine Camk2d start codon site by U6 promoterDepositorInsertMYL2, TadA, nSauriCas9
UseAAVTagsExpressionMutationPromoterMYL2Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDY2259 hNOLC1 atgRNA Paired Guide 1
Plasmid#220989PurposehNOLC1 atgRNADepositorInsertNOLC1 atgRNA (NOLC1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
RhoA gRNA #1
Plasmid#198718PurposeCas9-mediated knockout of RhoA in mammalian cells #1DepositorInsertRhoA (Rhoa Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
RhoC gRNA #1
Plasmid#198724PurposeCas9-mediated knockout of RhoC in mammalian cells #1DepositorInsertRhoC (Rhoc Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
RhoC gRNA #3
Plasmid#198726PurposeCas9-mediated knockout of RhoC in mammalian cells #3DepositorInsertRhoC (Rhoc Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nucGFP11-3xPax7gRNA
Plasmid#224570PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.DepositorInsertGFP11-H2B
UseCRISPRTagsHistone H2B (nuclear localization)ExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-3xGFP11-mem-3xPax7gRNA
Plasmid#224571PurposeUbiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.DepositorInsert3xGFP11
UseCRISPRTagsMembrane Localization SignalExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-U6-E690_guide-CBh-hSpCas9
Plasmid#188545PurposePlasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid E690DepositorInsertgRNA
UseCRISPRTagsT2A-EGFPExpressionMutationPromoterU6Available sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-U6-L101_guide-CBh-hSpCas9
Plasmid#188547PurposePlasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid L101DepositorInsertgRNA
UseCRISPRTagsT2A-EGFPExpressionMutationPromoterU6Available sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-hL1-gRNA1-EGFP
Plasmid#226002PurposeCRISPRi-KD of human LINE1DepositorInsertsgRNA targeting human L1HS
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Luciferase shRNA-TRE
Plasmid#225339PurposeshRNAmir backbone under tet-operator for RNA interference, with YFPDepositorInsertLuciferase shRNA (LOC116160065 Mouse)
UseAAV and RNAiTagsExpressionMammalianMutationPromoterTRE (tetracycline-responsive element)Available sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA242
Plasmid#215943PurposeFragmid fragment: (guide cassette) guide expression; positive control cell surfaceDepositorHas ServiceCloning Grade DNAInsertU6_v1; sgCD47_v1 [Sp]; trRNA_v4 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
UCK2_Deletion_gRNA_1
Plasmid#204672PurposeDual gRNA plasmid for UCK2 deletionDepositorInserturidine-cytidine kinase 2 (UCK2 Human)
UseLentiviralTagsExpressionMutationPromoterU6Available sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF827-sgNT-3_U6-sgRNA-EFS-Cas9-P2A-mNeonGreen
Plasmid#211695PurposeU6-sgRNA-EFS-Cas9-P2A-mNeonGreenDepositorInsertSpyCas9 and sgNT-3 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF827-sgNT-2_U6-sgRNA-EFS-Cas9-P2A-mNeonGreen
Plasmid#211694PurposeU6-sgRNA-EFS-Cas9-P2A-mNeonGreenDepositorInsertSpyCas9 and sgNT-2 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only