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CMV-dCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA

Plasmid

#171384

Purpose

Expression vector for encoding a human codon-optimized dCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

Depositor

Hui Yang

Article

Xu et al Nat Methods. 2021 May;18(5):499-506. doi

Insert

humanized dCas13X.1-RESCUE-S

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV, U6

Available since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ9757: CMV-BPNLS_TadA-linkerA-TadA*(8e)-Linker B-SV40 NLS-dCasMINI-V4-linkerC-c-Myc_NLS-3x FLAG-polyA

Plasmid

#176270

Purpose

Expression of TadA-TadA* (8e)-dCasMINI-V4 in mammalian cells

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

TadA-TadA*(8e)-dCasMINI-V4

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ202

Plasmid

#86198

Purpose

Multisite Gateway T-DNA entry plasmid (attR1 & attR2); AtUBQ10 promoter and Hygromiycin resistance marker

Depositor

Yiping Qi

Article

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Type

Empty backbone

Use

Gateway compatible attr1-attr2 destination vector

Tags

Expression

Plant

Mutation

Promoter

Arabidopsis ubiquitin 10 (AtUbi10)

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiCD19_H1_Gal4VP64

Plasmid

#169918

Purpose

Expression of a synNotch receptor containing antiCD19, a human Notch1 core, and Gal4VP64.

Depositor

Zhili Rong

Article

Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

Insert

antiCD19-Notch1-GL4VP64

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pPV-EF1a-mRxxFP1(AfeI)-iP-A

Plasmid

#204625

Purpose

Cloning vector for SSA vector (mRxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestion

Depositor

Akitsu Hotta

Article

Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pJEP300-pAAV-CMV-MCS2-pA

Plasmid

#112138

Purpose

CMV driven Multi Cloning Site-2

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

N/A

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-P-AtAFB2

Plasmid

#129715

Purpose

AAVS1 safe harbor targeting vector expressing AtAFB2

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

AFB2 (AFB2 Mustard Weed)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

no

Expression

Mammalian

Mutation

codon optimized

Promoter

EF1a

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti_gRNA -hMLHdn-Puro

Plasmid

#191105

Purpose

Backbone for lentiviral pegRNA library construction with hMLH1dn and Puro marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

hMLH1dn

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

I31 silent mutation (ATA to ATC) to remove early …

Promoter

EF-1a

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro-CAG-GCaMP6s

Plasmid

#120896

Purpose

Donor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene.

Depositor

Xiaojun Lian

Article

Jiang et al iScience. 2018 Oct 12;9:27-35. doi: 10.1

Insert

GCaMP6s

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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th-P2A-EGFP donor

Plasmid

#65562

Purpose

Intron targeting-mediated EGFP knockin at the zebrafish th locus.

Depositor

Jiu-lin Du

Article

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

th-P2A-EGFP (th Zebrafish)

Use

Zebrafish injection

Tags

Expression

Mutation

Promoter

none

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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pHS1184

Plasmid

#205965

Purpose

NZ_CP025815 His14-TwinStrep-bdSUMO tagged DinG HNH protein (E. coli codon optimized) in pET45b(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

DinG HNH

Use

Tags

14xHis-TwinStrep-bdSUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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th-P2A-Gal4 donor

Plasmid

#65563

Purpose

Intron targeting-mediated Gal4 knockin at the zebrafish th locus.

Depositor

Jiu-lin Du

Article

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

th-P2A-Gal4 (th Zebrafish)

Use

Zebrafish injection

Tags

Expression

Mutation

Promoter

none

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pHS1528

Plasmid

#205982

Purpose

U6-MWEE01000013 Cas5-HNH DR-BpiI-DR

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5-HNH DR-BpiI-DR

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiCD19_Z3_Gal4VP64

Plasmid

#169916

Purpose

Expression of a synNotch receptor containing antiCD19, a zebrafish Notch core and Gal4VP64.

Depositor

Zhili Rong

Article

Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

Insert

antiCD19-Notch3-GL4VP64

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL

Plasmid

#79614

Purpose

Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Inserts

SpCas9
PmCDA1

Use

Tags

SH3 domain and SHL

Expression

Yeast

Mutation

D10A and H840A for nuclease deficient Cas9

Promoter

pGal1 and pGal10

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pZ005 (AacC2c1 human codon optimized protein)

Plasmid

#70171

Purpose

Expresses AacC2c1 human codon optimized protein

Depositor

Feng Zhang

Article

Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

Insert

AacC2c1 protein

Use

Tags

NLS

Expression

Mutation

Promoter

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pJEP301-pAAV-CMV-MCS3-pA

Plasmid

#113676

Purpose

CMV driven Multi Cloning Site-3

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

N/A

Use

AAV

Tags

Expression

Mutation

Promoter

Cytomegalo Virus(CMV)

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)

Plasmid

#79619

Purpose

Expresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Use

Tags

3xFlag-PmCDA1

Expression

Mammalian

Mutation

D10A for nickase Cas9

Promoter

pCMV

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT)

Plasmid

#79621

Purpose

Expresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Use

Tags

PmCDA1

Expression

Mammalian

Mutation

D10A and H840A for nuclease deficient Cas9

Promoter

pCMV

Available since

Sept. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pJEP306-pAAV-EFS-MCS3-pA

Plasmid

#113682

Purpose

EFS driven Multi Cloning Site-3.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

N/A

Use

AAV

Tags

Expression

Mutation

Promoter

Cytomegalo Virus(CMV)

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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