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We narrowed to 2,616 results for: pCas

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Showing: 1361 - 1380 of 2616 results

  7. pEF1a-PEmax-P2A-GFP

    Plasmid
    #202000
    Purpose
    Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.
    Depositor
    Daniel Geschwind
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PEmax-P2A-GFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    202000-plasmid-map-sequence-id-395506

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  9. p101_CRISPRai_PiggyBac

    Plasmid
    #213777
    Purpose
    Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.
    Depositor
    Howard Chang
    Publication
    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    VPR-dSaCas9
    dSpCas9-KRAB-BFP
    zeocin
    Tet-On transactivator
    Use
    CRISPR
    Promoter
    EF1a (EF-1-alpha intron a) and Tet_On_TRE
    Available since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    213777-plasmid-map-sequence-id-431902

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  15. PE7_AAVS1_knockin_HDR_donor

    Plasmid
    #223001
    Purpose
    HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm
    Depositor
    Brittany Adamson
    Publication
    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE7
    Use
    Hdr donor
    Tags
    SV40 bpNLS and c-Myc NLS
    Expression
    Mammalian
    Promoter
    EF1α
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    223001-plasmid-map-sequence-id-442579

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  17. Eef1a-1955/-1>nls::Cas9::nls

    Plasmid
    #59987
    Purpose
    Eef1a (EF1alpha) promoter driving nls::Cas9::nls
    Depositor
    Lionel Christiaen
    Publication
    Stolfi et al Development. 2014 Nov;141(21):4115-20. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nls::Cas9::nls
    Use
    CRISPR
    Mutation
    Reverted mutations from dCas9 to wiltdtype
    Promoter
    Ciinte.Eef1a (EF1alpha) -1955/-1
    Available since
    Nov. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    59987-plasmid-map-sequence-id-95666

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  18. pHis-parallel1-NLSH2BCas9

    Plasmid
    #112065
    Purpose
    Expression and purification of NLSH2BCas9 for fungal gene editing
    Depositor
    Jeff Coleman
    Publication
    Wang et al Fungal Genet Biol. 2018 May 12. pii: S10
    Insert
    NLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger
    Use
    CRISPR
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Cas9 was codon optimized for Aspergillus niger
    Promoter
    promoter in vector
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112065-plasmid-map-sequence-id-217190

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Showing: 1361 - 1380 of 2616 results