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Plasmid#188156PurposeMammalian Expression Plasmid of anti-IRSp53/BAIAP2 (Human). Derived from hybridoma L117/52DepositorInsertanti-IRSp53/BAIAP2 (Homo sapiens) recombinant mouse monoclonal antibody (BAIAP2 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
M71 pGL3 Sp5l P1203 mut
Plasmid#17209DepositorInsertSp5l promoter (sp5l Zebrafish)
UseLuciferaseTagsluciferaseExpressionMutation6 of the putative Tcf/Lef sites mutatedPromoterAvailable sinceMay 18, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBac[3xP3-EGFP;Tc'hsp5'-Gal4Delta-3'UTR]
Plasmid#86449PurposepBAc transposon with transformation marker for Tribolium transgenesis; Drives EGFP expression in the eyes; contains Gal4_ driven by Tribolium heatshock promoter; (Transformation plasmid)DepositorInsert3xP3-EGFP;Tc'hsp5'-Gal4Delta-3'UTR
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLIB_3xFlag-His-nsp5CS-nsp9 (SARS-CoV-2)
Plasmid#169177PurposeBaculoviral transfer vector to express SARS-CoV-2 nsp9 with nsp5-cleavable 3xFlag-His6 tag in insect cellsDepositorInsert3xFlag-His-nsp5CS-nsp9 (ORF1ab SARS-CoV-2)
UseTags3xFlag-His6 (nsp5-cleavable)ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-IRSp53-T2A-Rac1(G12V)
Plasmid#221330PurposeDox-inducible expression of FLAG-IRSp53-T2A-HA-Rac1 G12V in mammalian cells by retroviral transductionDepositorUseRetroviralTagsExpressionMutationG12VPromoterTRE3GAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-IRSp53-T2A-Cdc42(G12V)
Plasmid#221329PurposeDox-inducible expression of FLAG-IRSp53-T2A-HA-Cdc42 G12V in mammalian cells by retroviral transductionDepositorUseRetroviralTagsExpressionMutationG12VPromoterTRE3GAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-STING L373A with IRSp53 pLxIS
Plasmid#221283PurposeExpression of STING L373A with IRSp53 pLxIS (RLL361NLV) in mammalian cells by retroviral transductionDepositorUseRetroviralTags3xFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTV-Usp5-5prime-1000-24MS96T-3F_NeoR
Plasmid#174885PurposeTargeting vector for generating Usp5 STREAMING-tag KI cellDepositorInsertSTREAMING-tag with homology arms for Usp5
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-nsp5-2xStrep-IRES-Puro
Plasmid#141370PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp5 (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-EF1alpha-SARS-CoV-2-nsp5-C145A-2xStrep-IRES-Puro
Plasmid#141371PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp5-C145A (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationC145A, human codon optimizedPromoterEF1alphaAvailable sinceApril 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
ZP59 23f17
Plasmid#16883DepositorInsert23f17
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
ZP62 Cystathionine beta-synthase
Plasmid#16880DepositorInsertcystathionine beta-synthase (cbsb Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
ZP61 Isthmin
Plasmid#16881DepositorInsertisthmin (ism1 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
ZP60 Axin2
Plasmid#16882DepositorInsertaxin2 (axin2 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
TFORF0037
Plasmid#141488PurposeLentiviral vector for overexpressing the SP5 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSP5 (SP5 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
HyWnt3:Luc
Plasmid#192986PurposePlasmid to test the Hydra Wnt3 promoter activityDepositorInsertHydra Wnt3 promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
ZfWnt3:Luc
Plasmid#192992PurposePlasmid to test the Zebrafish Wnt3 promoter activityDepositorInsertZebrafish Wnt3 promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
HyWnt3-ΔRep:Luc
Plasmid#192987PurposePlasmid to test the Hydra Wnt3 promoter activity (full deletion of the repressor element)DepositorInsertHydra Wnt3 promoter
UseTagsExpressionMammalianMutationRepressor element deletedPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
HyWnt3-ΔRep-D3:Luc
Plasmid#192990PurposePlasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D3)DepositorInsertHydra Wnt3 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Wnt3 repressor element-D3PromoterAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
HyWnt3-ΔRep-D1:Luc
Plasmid#192988PurposePlasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D1)DepositorInsertHydra Wnt3 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Wnt3 repressor element-D1PromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only