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OmpX

Plasmid

#213136

Purpose

Expresses outer membrane protein X

Depositor

Sebastian Hiller

Article

Rath et al Biochim Biophys Acta Biomembr. 2020 Jan

Insert

OmpX (24-171) (ompX E.coli (K12))

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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InterTag-BFP

Plasmid

#186901

Purpose

Cell-free expression of transmembrane protein PDGFR-tagBFP-sfCherry11-SpyTag

Depositor

Taekjip Ha, Allen Liu

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

SpyTag003-sfCherry11-tagBFP-PDGFR

Use

Synthetic Biology

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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A3RXB7

Plasmid

#177694

Purpose

expresses a N-acetyl hexosamine oxidase in E. coli

Depositor

Martin Engqvist

Article

Rembeza et al Chembiochem. 2022 Jan 19;23(2):e20210051

Insert

N-acetylhexosamine oxidase

Tags

10xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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PNCA-mCherry

Plasmid

#67790

Purpose

Expression of MMLV proviral DNA-mCherry. Use together with pCMV-intron and VSV-G to package MMLV reporter virus

Depositor

Stephen Goff

Article

Schlesinger et al EMBO Rep. 2013 Jan;14(1):73-9. doi: 10.1

Insert

MMLV-LTR-mCherry

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
pMA_Auxiliary 1

Plasmid

#102709

Purpose

Provides the Middle-to-End linker 1 which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMA_Auxiliary 2

Plasmid

#102710

Purpose

Provides the Middle-to-End linker 2 which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMA_Auxiliary 3

Plasmid

#102711

Purpose

Provides the Middle-to-End linker 3 which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMA_Auxiliary 4A

Plasmid

#102712

Purpose

Provides the End-to-End linker 4A which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMA_Auxiliary 4Γ

Plasmid

#102714

Purpose

Provides the End-to-End linker 4Γ which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMA_Auxiliary 4Δ

Plasmid

#102715

Purpose

Provides the End-to-End linker 4Δ which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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mUAV

Plasmid

#102680

Purpose

Mobius Assembly Universal Acceptor Vector, it converts amplified PCR fragments into standard, exchangeable parts

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

J23106:B0034:amilCP:rrnBT1-T7TE

Use

Synthetic Biology

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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p1990 SV40-LMP1

Plasmid

#26654

Depositor

Bill Sugden

Article

Kaykas et al EMBO J. 2001 Jun 1. 20(11):2641-54.

Insert

Latent Membrane Protein 1 (LMP-1 Epstein-Barr Virus)

Expression

Mammalian

Available Since

April 27, 2012

Availability

Academic Institutions and Nonprofits only

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pMA_Auxiliary 4B

Plasmid

#102713

Purpose

Provides the End-to-End linker 4B which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMSP2N2

Plasmid

#29520

Depositor

Stephen Sligar

Article

Grinkova et al Protein Eng Des Sel. 2010 Nov . 23(11):8

Insert

MSP2N2

Tags

7-His tag

Expression

Bacterial

Mutation

tandem MSP; His-tag on N-terminus followed by spa…

Available Since

May 3, 2011

Availability

Academic Institutions and Nonprofits only

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pGL4.26_4xUAS_DSCP_GFP

Plasmid

#111931

Purpose

Vector to express GFP (under the control of the DSCP and an array of 4 UAS sites)

Depositor

Alexander Stark

Article

Arnold et al EMBO J. 2018 Aug 15;37(16). pii: embj.20

Insert

4xUAS GFP

Expression

Insect

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH_blast_MCS_Nard_GFP_D50

Plasmid

#167339

Purpose

Expresses GFP fused to progerin (d50) in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

Progerin

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

50AA deletion creating Progerin instead of mature…

Promoter

CMV

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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A0A075HNX4

Plasmid

#177695

Purpose

expresses a fatty alcohol oxidase in E. coli

Depositor

Martin Engqvist

Article

Rembeza et al Chembiochem. 2022 Jan 19;23(2):e20210051

Insert

Long chain alcohol oxidase

Tags

10xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pccGFP-MalE-pos-2H01

Plasmid

#239760

Purpose

Positive control for liquid MalE complementation assay

Depositor

Alessandro Senes

Article

Anderson et al bioRxiv 2025.04.22.650048

Insert

transmembrane domain of SEM5A

Tags

MBP and ToxR

Expression

Bacterial

Mutation

Changed Leucine 985 to Isoleucine

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pccGFP-MalE-neg-2H11

Plasmid

#239086

Purpose

Negative control for liquid MalE complementation assay

Depositor

Alessandro Senes

Article

Anderson et al bioRxiv 2025.04.22.650048

Insert

transmembrane domain of EVA1B

Tags

MBP and ToxR

Expression

Bacterial

Mutation

Changed Leucine 30 to Isoleucine

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pccGFP-MalE-pos-1F02

Plasmid

#239087

Purpose

Positive control for liquid MalE complementation assay

Depositor

Alessandro Senes

Article

Anderson et al bioRxiv 2025.04.22.650048

Insert

transmembrane domain of AMPE

Tags

MBP and ToxR

Expression

Bacterial

Mutation

deleted amino acids 19-22, and changed Leucine 35…

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pccGFP-MalE-pos-2H07

Plasmid

#239088

Purpose

Positive control for liquid MalE complementation assay

Depositor

Alessandro Senes

Article

Anderson et al bioRxiv 2025.04.22.650048

Insert

transmembrane domain of FND10

Tags

MBP and ToxR

Expression

Bacterial

Mutation

Changed Valine 184 to Isoleucine

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pccGFP-MalE-neg-2E11

Plasmid

#239084

Purpose

Negative control for liquid MalE complementation assay

Depositor

Alessandro Senes

Article

Anderson et al bioRxiv 2025.04.22.650048

Insert

transmembrane domain of LRIG3

Tags

MBP and ToxR

Expression

Bacterial

Mutation

Changed Valine 827 to Isoleucine

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pccGFP-MalE-neg-2F12

Plasmid

#239085

Purpose

Negative control for liquid MalE complementation assay

Depositor

Alessandro Senes

Article

Anderson et al bioRxiv 2025.04.22.650048

Insert

transmembrane domain of LRIG3

Tags

MBP and ToxR

Expression

Bacterial

Mutation

Changed Valine 815 to Isoleucine

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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PB-Blast-Dest-LifeAct-mRuby

Plasmid

#194907

Purpose

To generate LifeAct-mRuby expressing stable cell lines with piggybac transposase co-expression and blastocidin selections.

Depositor

Clare Buckley

Article

Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

Insert

LifeAct

Use

Mouse Targeting

Tags

mRuby

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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C1-MPAct-mRuby3

Plasmid

#155221

Purpose

Monitor changes in the density of membrane-proximal F-actin (MPA)

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b

Tags

mRuby3

Expression

Mammalian

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Blast NT shRNA

Plasmid

#123306

Purpose

Expression of a non-targeting shRNA

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

Non-targeting shRNA

Use

Lentiviral and RNAi

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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p-mCherry2-sgRNA (empty)

Plasmid

#198330

Purpose

Customisable sgRNA sequence with optimised sgRNA scaffold for expression under U6 promoter, with mCherry2 reporter

Depositor

Sarah McClelland

Article

Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

Insert

U6-sgRNA(F+E) empty

Expression

Mammalian

Promoter

U6

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-cMSP26

Plasmid

#199769

Purpose

Expresses split-intein fused MSP26 for circularization during expression (internal His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSP26

Tags

His6-Tag

Expression

Bacterial

Mutation

internal His-Tag

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-cMSPdH4-6

Plasmid

#199766

Purpose

Expresses split-intein fused MSPdH4-6 for circularization during expression (internal His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSPdH46

Tags

His6-Tag

Expression

Bacterial

Mutation

internal His-Tag

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-cMSP1D1

Plasmid

#199768

Purpose

Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSP1D1

Tags

His6-Tag

Expression

Bacterial

Mutation

external His-Tags flanking the C and N termini

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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C1-MPAct-mCherry

Plasmid

#155222

Purpose

Monitor changes in the density of membrane-proximal F-actin (MPA)

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b

Tags

mCherry

Expression

Mammalian

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
C1-MPAct-mCitrine

Plasmid

#155220

Purpose

Monitor changes in the density of membrane-proximal F-actin (MPA)

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with the CaaX sequence derived from Kras4b

Tags

mCitrine

Expression

Mammalian

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-cMSPdH5

Plasmid

#199771

Purpose

Expresses split-intein fused MSPdH5 for circularization during expression (external His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSPdH5

Tags

His6-Tag

Expression

Bacterial

Mutation

external His-Tags flanking the C and N termini

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-cMSPdH45

Plasmid

#199767

Purpose

Expresses split-intein fused MSPdH45 for circularization during expression (internal His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSPdH45

Tags

His6-Tag

Expression

Bacterial

Mutation

internal His-Tag

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TRAPa-3xFLAG-pCDNA

Plasmid

#201963

Purpose

Mammalian expression of Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Tags

3xFLAG-tag

Expression

Mammalian

Available Since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pML2(−)WF

Plasmid

#108337

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pML2(+)BR

Plasmid

#108336

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pML2(+)WF

Plasmid

#108333

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pML2(+)BF

Plasmid

#108334

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pML2(+)WR

Plasmid

#108335

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 17,519 results for: Emb