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Showing: 241 - 280 of 798 results

  11. pET28-MBP-super TEV protease

    Plasmid
    #171782
    Purpose
    Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    MBP-sTEV
    Tags
    Arg6 tag and Internal His6 tag
    Expression
    Bacterial
    Mutation
    No internal TEV cleavage site between the MBP and…
    Promoter
    T7
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171782-plasmid-map-sequence-id-443047

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  12. Negative Control Binder

    Plasmid
    #179140
    Purpose
    Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9) and an additionional A70Q mutation greatly reduces affinity for SsrA peptide
    Depositor
    Klaus Hahn
    Article
    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.
    Insert
    SspB
    Tags
    FLAG and mCherry
    Expression
    Mammalian
    Mutation
    Y11K/A15E, residue numbering from pdb: 1ou9. Addi…
    Available Since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    179140-plasmid-map-sequence-id-353410

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  13. pDEST-N2H-N1

    Plasmid
    #125547
    Purpose
    Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment1
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment1 attached to n-terminus of Gatew…
    Expression
    Bacterial and Mammalian
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125547-plasmid-map-sequence-id-254706

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  14. pDEST-N2H-C1

    Plasmid
    #125549
    Purpose
    Gateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment1
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment1 attached to c-terminus of Gatew…
    Expression
    Mammalian and Yeast
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125549-plasmid-map-sequence-id-254837

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  15. pDEST-N2H-C2

    Plasmid
    #125559
    Purpose
    Gateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment2
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment2 attached to c-terminus of Gatew…
    Expression
    Mammalian and Yeast
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125559-plasmid-map-sequence-id-254701

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  16. pDEST-N2H-N2

    Plasmid
    #125548
    Purpose
    Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment2
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment2 attached to n-terminus of Gatew…
    Expression
    Mammalian and Yeast
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125548-plasmid-map-sequence-id-254836

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  17. pFUSEss-CHIg-mM-Asn209Asp_M18

    Plasmid
    #91781
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Asp (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Asp (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91781-plasmid-map-sequence-id-181014

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  18. pFUSEss-CHIg-mM-Asn209Ala_M18

    Plasmid
    #91733
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ala (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Ala (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    June 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    91733-plasmid-map-sequence-id-180936

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  19. pTT5-hSDC1-S15A

    Plasmid
    #52363
    Purpose
    expresses human Syndecan-1 serine 15 mutated to alanine (numbering excludes signal peptide) in HEK293-EBNA1 (293E) suspension culture.
    Depositor
    Gordon Laurie
    Article
    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1
    Insert
    SDC1 (SDC1 Human)
    Expression
    Mammalian
    Mutation
    S15A and R95Q (according to numbering in Fig. 2A …
    Promoter
    CMV
    Available Since
    Dec. 8, 2014
    Availability
    Academic Institutions and Nonprofits only

  21. pFUSEss-CHIg-mM-Arg210Ala_M18

    Plasmid
    #91734
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Arg210Ala (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Arg210 changed to Ala (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    Oct. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    91734-plasmid-map-sequence-id-194569

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  22. pFUSEss-CHIg-mM-Asn209Arg_M18

    Plasmid
    #91740
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Arg (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Arg (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    91740-plasmid-map-sequence-id-191795

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  23. pFUSEss-CHIg-mM-Asn209Ile_M18

    Plasmid
    #91746
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ile (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Ile (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    91746-plasmid-map-sequence-id-190060

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  24. pFUSEss-CHIg-mM-Asn209Val_M18

    Plasmid
    #91756
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Val (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Val (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    91756-plasmid-map-sequence-id-190059

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  25. pFUSEss-CHIg-mM-Asn209Tyr_M18

    Plasmid
    #91755
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Tyr (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Tyr (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91755-plasmid-map-sequence-id-182657

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  26. pFUSEss-CHIg-mM-Asn209Thr_M18

    Plasmid
    #91753
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Thr (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Thr (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91753-plasmid-map-sequence-id-182656

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  27. pFUSEss-CHIg-mM-Lys208Ala_M18

    Plasmid
    #91732
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Lys208Ala (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Lys208 changed to Ala (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91732-plasmid-map-sequence-id-182642

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  28. pFUSEss-CHIg-mM-Asn209Trp_M18

    Plasmid
    #91754
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Trp (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Trp (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    June 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91754-plasmid-map-sequence-id-181130

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  29. pFUSEss-CHIg-mM-Asn209Ser_M18

    Plasmid
    #91752
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ser (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Ser (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91752-plasmid-map-sequence-id-181013

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  30. pFUSEss-CHIg-mM-Asn209Pro_M18

    Plasmid
    #91751
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Pro (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Pro (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91751-plasmid-map-sequence-id-181012

    Enlarge

  31. pFUSEss-CHIg-mM-Asn209Phe_M18

    Plasmid
    #91750
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Phe (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Phe (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91750-plasmid-map-sequence-id-181011

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  32. pFUSEss-CHIg-mM-Asn209Met_M18

    Plasmid
    #91749
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Met (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Met (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91749-plasmid-map-sequence-id-181009

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  33. pFUSEss-CHIg-mM-Asn209Lys_M18

    Plasmid
    #91748
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Lys (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Lys (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91748-plasmid-map-sequence-id-181008

    Enlarge

  34. pFUSEss-CHIg-mM-Asn209Leu_M18

    Plasmid
    #91747
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Leu (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Leu (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91747-plasmid-map-sequence-id-181007

    Enlarge

  35. pFUSEss-CHIg-mM-Asn209His_M18

    Plasmid
    #91745
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209His (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to His (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91745-plasmid-map-sequence-id-181006

    Enlarge

  36. pFUSEss-CHIg-mM-Asn209Gly_M18

    Plasmid
    #91744
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Gly (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Gly (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91744-plasmid-map-sequence-id-181005

    Enlarge

  37. pFUSEss-CHIg-mM-Asn209Glu_M18

    Plasmid
    #91743
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Glu (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Glu (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91743-plasmid-map-sequence-id-181003

    Enlarge

  38. pFUSEss-CHIg-mM-Asn209Gln_M18

    Plasmid
    #91742
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Gln (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Gln (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91742-plasmid-map-sequence-id-181002

    Enlarge

  39. pFUSEss-CHIg-mM-Asn209Cys_M18

    Plasmid
    #91741
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Cys (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Cys (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91741-plasmid-map-sequence-id-180999

    Enlarge

  40. pFUSEss-CHIg-mM-His214Ala_M18

    Plasmid
    #91737
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: His214Ala (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    His214 changed to Ala (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91737-plasmid-map-sequence-id-180997

    Enlarge

Showing: 241 - 280 of 798 results