Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 739 results for: Numb

Showing: 241 - 280 of 739 results

  1. pFUSEss-CHIg-mM-Asn209Phe_M18

    Plasmid
    #91750
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Phe (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Phe (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91750-plasmid-map-sequence-id-181011

    Enlarge

  2. pFUSEss-CHIg-mM-Asn209Met_M18

    Plasmid
    #91749
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Met (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Met (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91749-plasmid-map-sequence-id-181009

    Enlarge

  3. pFUSEss-CHIg-mM-Asn209Lys_M18

    Plasmid
    #91748
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Lys (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Lys (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91748-plasmid-map-sequence-id-181008

    Enlarge

  4. pFUSEss-CHIg-mM-Asn209Leu_M18

    Plasmid
    #91747
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Leu (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Leu (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91747-plasmid-map-sequence-id-181007

    Enlarge

  5. pFUSEss-CHIg-mM-Asn209His_M18

    Plasmid
    #91745
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209His (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to His (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91745-plasmid-map-sequence-id-181006

    Enlarge

  6. pFUSEss-CHIg-mM-Asn209Gly_M18

    Plasmid
    #91744
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Gly (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Gly (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91744-plasmid-map-sequence-id-181005

    Enlarge

  7. pFUSEss-CHIg-mM-Asn209Glu_M18

    Plasmid
    #91743
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Glu (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Glu (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91743-plasmid-map-sequence-id-181003

    Enlarge

  8. pFUSEss-CHIg-mM-Asn209Gln_M18

    Plasmid
    #91742
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Gln (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Gln (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91742-plasmid-map-sequence-id-181002

    Enlarge

  9. pFUSEss-CHIg-mM-Asn209Cys_M18

    Plasmid
    #91741
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Cys (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Cys (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91741-plasmid-map-sequence-id-180999

    Enlarge

  10. pFUSEss-CHIg-mM-His214Ala_M18

    Plasmid
    #91737
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: His214Ala (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    His214 changed to Ala (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91737-plasmid-map-sequence-id-180997

    Enlarge

  11. pFUSEss-CHIg-mM-Leu213Ala_M18

    Plasmid
    #91736
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Leu213Ala (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Leu213 changed to Ala (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91736-plasmid-map-sequence-id-180995

    Enlarge

  12. pFUSEss-CHIg-mM-Asp212Ala_M18

    Plasmid
    #91735
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asp212Ala (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asp212 changed to Ala (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    91735-plasmid-map-sequence-id-180938

    Enlarge

  13. pFUSEss-CHIg-mM-Tyr205Ala_M18

    Plasmid
    #91731
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Tyr205Ala (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Tyr205 changed to Ala (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    91731-plasmid-map-sequence-id-180935

    Enlarge

  14. pFUSEss-CHIg-mM-His204Ala_M18

    Plasmid
    #91730
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: His204Ala (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    His204 changed to Ala (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    91730-plasmid-map-sequence-id-180933

    Enlarge

  15. pTT5-hSDC1-S15/25A

    Plasmid
    #52364
    Purpose
    expresses human Syndecan-1 serine 15 and 25 mutated to alanine (numbering excludes signal peptide) in HEK293-EBNA1 (293E) suspension culture.
    Depositor
    Gordon Laurie
    Article
    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1
    Insert
    SDC1 (SDC1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S15A, S25A and R95Q (according to numbering in Fi…
    Promoter
    CMV
    Available since
    Dec. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52364-plasmid-map-sequence-id-136249

    Enlarge

  16. pTT5-hSDC1-N20

    Plasmid
    #52355
    Purpose
    expresses human syndecan 1 'del 1- 20' truncation mutant in HEK293-EBNA1 (293E) suspension culture.
    Depositor
    Gordon Laurie
    Article
    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1
    Insert
    SDC1 (SDC1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of SDC1 aa2-21 (according to numbering i…
    Promoter
    CMV
    Available since
    Dec. 8, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pDEST-N2H-F2-empty vector

    Plasmid
    #125552
    Purpose
    N2H assay vector with nanoluc fragment2 (empty control, no Gateway cloning site, expressing nanoluc fragment2)
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment2 alone without any attached prot…
    Expression
    Mammalian and Yeast
    Mutation
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125552-plasmid-map-sequence-id-252313

    Enlarge

  18. pDEST-N2H-F1-empty vector

    Plasmid
    #125551
    Purpose
    N2H assay vector with nanoluc fragment1 (empty control, no Gateway cloning site, expressing nanoluc fragment1)
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment1 alone without any attached prot…
    Expression
    Mammalian and Yeast
    Mutation
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125551-plasmid-map-sequence-id-252311

    Enlarge

  19. pFUSEss-chimeric-IgM-mouse/human_M18

    Plasmid
    #91738
    Purpose
    Expression plasmid coding for modified heavy chain of mouse IgM antibody. Residues 203-239 (EU numbering) were exchanged to human homologue sequence.
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Residues 203-239 (EU numbering) were exchanged to…
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91738-plasmid-map-sequence-id-181015

    Enlarge

  20. pFUSEss-CHIg-mM-Asp212Ser_M18

    Plasmid
    #91739
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asp212Ser (EU numbering) - N-glycosylated Asn209
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asp212 changed to Ser (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91739-plasmid-map-sequence-id-180998

    Enlarge

  21. pBluescript-PB

    Plasmid
    #133885
    Purpose
    piggyBac ITR's in high copy number pBluescript backbone plasmid
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Piggybac
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    133885-plasmid-map-sequence-id-274419

    Enlarge

  22. p15A-PB

    Plasmid
    #133886
    Purpose
    piggyBac ITR's in low copy number p15A backbone plasmid
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Piggybac
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    133886-plasmid-map-sequence-id-274420

    Enlarge

  23. pBluescript-minimal PB

    Plasmid
    #177151
    Purpose
    Minimal piggyBac ITR's in high copy number pBluescript backbone plasmid
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Piggybac
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    177151-plasmid-map-sequence-id-348859

    Enlarge

  24. pUMN105

    Plasmid
    #233024
    Purpose
    Encodes eGFP for ORBIT integration. High copy number.
    Depositor
    Anthony Baughn
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    233024-plasmid-map-sequence-id-467921

    Enlarge

  25. C1m

    Plasmid
    #120955
    Purpose
    MoClo golden gate assembly CD part for deGFP (deGFP3 with 4aa N-truncation, 9aa C-truncation, F64L, and C203T (numbered with original sequence); see https://www.fpbase.org/protein/degfp3/)
    Depositor
    Richard Murray
    Insert
    deGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120955-plasmid-map-sequence-id-235968

    Enlarge

  26. pSAH031

    Plasmid
    #90330
    Purpose
    Promoterless iLOV gene for recombineering, flanked by BsmbI restriction sites.
    Depositor
    David Savage
    Insert
    iLOV
    Use
    Tags
    None
    Expression
    Mutation
    Promoter
    None
    Available since
    July 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    90330-plasmid-map-sequence-id-189687

    Enlarge

  27. pKP2186

    Plasmid
    #167637
    Purpose
    Express FepA T13C, I14P . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Insert
    fepA T13C, I14P
    Use
    Tags
    Expression
    Bacterial
    Mutation
    fepA with T13C, I14P mutations (Mutations are T3…
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167637-plasmid-map-sequence-id-339561

    Enlarge

  28. pKP2187

    Plasmid
    #167640
    Purpose
    Express FepA V15C, I14P . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Insert
    fepA V15C, I14P
    Use
    Tags
    Expression
    Bacterial
    Mutation
    fepA with V15C, I14P mutations (Mutations are I36…
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167640-plasmid-map-sequence-id-339573

    Enlarge

  29. pKP2189

    Plasmid
    #167644
    Purpose
    Express FepA T17C, I14P . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Insert
    fepA T17C, I14P
    Use
    Tags
    Expression
    Bacterial
    Mutation
    fepA with T17C, I14P mutations (Mutations are I36…
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167644-plasmid-map-sequence-id-339582

    Enlarge

  30. pKP2185

    Plasmid
    #167635
    Purpose
    Express FepA D12C, I14P . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Insert
    fepA D12C, I14P
    Use
    Tags
    Expression
    Bacterial
    Mutation
    fepA with D12C, I14P mutations (Mutations are D34…
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167635-plasmid-map-sequence-id-339489

    Enlarge

  31. pLNKR1

    Plasmid
    #67968
    Purpose
    High copy number plasmid encoding part of the tra Operon PY-traR
    Depositor
    Joshua Leonard
    Insert
    tra Operon (First Quarter)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    67968-plasmid-map-sequence-id-121285

    Enlarge

  32. pB6-Hipp11-DTA-Capecchi-for

    Plasmid
    #126649
    Purpose
    B6J Hipp11 targeting vector homology arms, Capecchi configuration, DTA, I-CeuI, forward cloning sites, low copy number
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    126649-plasmid-map-sequence-id-247127

    Enlarge

  33. pJM104: pTrc RFP on pCM66T

    Plasmid
    #74745
    Purpose
    pCM66T with promoter pTrc driving RFP expression (maxRFP = BBa_E1010)
    Depositor
    Mary Lidstrom
    Insert
    RFP (BioBrick part number BBa_E1010)
    Use
    Tags
    Expression
    Mutation
    Promoter
    pTrc
    Available since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    74745-plasmid-map-sequence-id-141461

    Enlarge

  34. pDTA-5

    Plasmid
    #128845
    Purpose
    Gateway-compatible p15A origin plasmid with a DTA negative selection cassette for targeting vector construction, I-CeuI, low copy number
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    128845-plasmid-map-sequence-id-252002

    Enlarge

  35. pB6-ROSA26-DTA-Capecchi-for

    Plasmid
    #125743
    Purpose
    B6J ROSA26 targeting vector homology arms, Capecchi configuration, DTA, I-CeuI, forward cloning sites, low copy number
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125743-plasmid-map-sequence-id-247902

    Enlarge

  36. pB6-Hipp11-DTA-Capecchi-rev

    Plasmid
    #127054
    Purpose
    B6J Hipp11 targeting vector homology arms, Capecchi configuration, DTA, I-CeuI, reverse cloning sites, low copy number
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    127054-plasmid-map-sequence-id-247133

    Enlarge

  40. pLNKR2

    Plasmid
    #67969
    Purpose
    High copy number plasmid encoding part of the tra Operon traC-artA
    Depositor
    Joshua Leonard
    Insert
    tra Operon (second Quarter)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    67969-plasmid-map-sequence-id-121291

    Enlarge

Showing: 241 - 280 of 739 results