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We narrowed to 22,196 results for: ESC

Showing: 301 - 350 of 22196 results

  1. pAAV-hSyn-sbGLuc-CheRiff-EGFP

    Plasmid
    #114108
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, synthetic channelrhodopsin CheRiff, and enhanced green fluorescent protein for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Ikefuama et al Int J Mol Sci. 2022 Oct 27;23(21):12994.
    Insert
    Gaussia luciferase, slow burn; CheRiff synthetic ChR; EGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available Since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    114108-plasmid-map-sequence-id-252563

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  2. pAAV-Hb9-LMO3 (sbGLuc-VChR1-EYFP)

    Plasmid
    #114103
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein (luminopsin LMO3) for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Petersen et al Front Neurol. 2022 Jan 20;12:792643. doi
    Insert
    sbGLuc-VChR1-EYFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    motoneuron-specific Homeobox 9 promoter
    Available Since
    Aug. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    114103-plasmid-map-sequence-id-252555

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  3. pAAV-hSyn-iLMO (slGLuc-Mac-EYFP)

    Plasmid
    #114100
    Purpose
    fusion protein of Gaussia luciferase variant superluminescent, Leptosphaeria maculans opsin, and enhanced yellow fluorescent protein for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11
    Insert
    Gaussia luciferase, superluminescent; Leptosphaeria maculans opsin; EYFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    114100-plasmid-map-sequence-id-252513

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  7. pGL001

    Plasmid
    #115674
    Purpose
    Fluorescent reporter for E.coli K12 fliC promoter activity
    Depositor
    Gene-Wei Li
    Article
    Parker et al J Bacteriol. 2019 Sep 6;201(19). pii: JB
    Insert
    fliC promoter fused with GFP
    Use
    Tags
    Green fluorescent protein (GFP)
    Expression
    Bacterial
    Mutation
    Promoter
    fliC promoter
    Available Since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    115674-plasmid-map-sequence-id-225632

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  16. pET30b_T7Sav

    Plasmid
    #138588
    Purpose
    T7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 ori
    Depositor
    Markus Jeschek
    Article
    Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10
    Insert
    T7-tagged streptavidin variant with native C-terminus
    Use
    Synthetic Biology
    Tags
    T7-tag
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available Since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    138588-plasmid-map-sequence-id-274485

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  18. pCKmatBC

    Plasmid
    #138587
    Purpose
    matBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, cat
    Depositor
    Markus Jeschek
    Article
    Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10
    Insert
    bicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    matB contains native GUG start codon
    Promoter
    Plac promoter
    Available Since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    138587-plasmid-map-sequence-id-274478

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  27. pmKalama1-Nuc

    Plasmid
    #14894
    Purpose
    Mammalian expression of mKalama1 blue fluorescent protein with NLS
    Depositor
    Robert Campbell
    Article
    Ai et al Biochemistry. 2007 May 22;46(20):5904-10
    Insert
    pmKalama1-Nuc
    Use
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    I178T mismatch, replace EYFP of the vector pEYFP-…
    Promoter
    Available Since
    May 15, 2007
    Availability
    Academic Institutions and Nonprofits only

  28. pH2B-SCFP1-2A-mTq2-NES

    Plasmid
    #60491
    Purpose
    Co-expresses two cyan fluorescent protein variants, a short fluorescence lifetime CFP variant in the nucleus and a CFP variant with a long lifetime (mTurquoise2) in the cytoplasm
    Depositor
    Dorus Gadella
    Article
    Joosen et al J Microsc. 2014 Sep 1. doi: 10.1111/jmi.
    Inserts
    H2B-SCFP1
    mTurquoise2-NES
    Use
    Tags
    SCFP1 and mTurquoise2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60491-plasmid-map-sequence-id-96973

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  36. 3xNLS-SGFP2(T65G)-T2A-SGFP2-3xNES

    Plasmid
    #60492
    Purpose
    Co-expresses two green fluorescent protein variants, a short fluorescence lifetime GFP variant in the nucleus and a GFP variant with a long lifetime in the cytoplasm
    Depositor
    Dorus Gadella
    Article
    Joosen et al J Microsc. 2014 Sep 1. doi: 10.1111/jmi.
    Inserts
    3xnls-SGFP2(T65G)
    SGFP2-3xNES
    Use
    Tags
    SGFP2 and SGFP2(T65G)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60492-plasmid-map-sequence-id-96974

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  37. pJC30X-BS(X)

    Plasmid
    #58958
    Purpose
    Encodes synthetic mitochondrial gene GFPm-3 in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.
    Depositor
    Thomas Fox
    Article
    Cohen et al Mitochondrion. 2001 Aug;1(2):181-9.
    Insert
    Enhanced fluorescence variant of GFP, in S. cerevisiae mitochondrial genetic code
    Use
    Tags
    First 8 codons of COX3
    Expression
    Mutation
    GFP modified as described: CORMACK, B. P., R. H. …
    Promoter
    n/a
    Available Since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  38. CMV-Y-GECO1

    Plasmid
    #55766
    Purpose
    Encoded a yellow fluorescent calcium ion indicator with inverted fluorescence response for expression in mammalian cells
    Depositor
    Robert Campbell
    Article
    Zhao et al Integr Biol (Camb). 2014 May 19.
    Insert
    Y-GECO1.0
    Use
    Tags
    6x His tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    July 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    55766-plasmid-map-sequence-id-89442

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  39. pTorPE-Y-GECO1

    Plasmid
    #55765
    Purpose
    Encoded a yellow fluorescent calcium ion indicator with inverted fluorescence response for expression in bacterial cells
    Depositor
    Robert Campbell
    Article
    Zhao et al Integr Biol (Camb). 2014 May 19.
    Insert
    Y-GECO1.0
    Use
    Lab constructed
    Tags
    6x His tag
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available Since
    July 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    55765-plasmid-map-sequence-id-89444

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  44. pEcRNAP6

    Plasmid
    #128940
    Purpose
    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, Z
    Depositor
    Seth Darst
    Article
    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:
    Inserts
    RpoA (rpoA E. coli)
    RpoB
    RpoC-ppx(His)10
    RpoZ
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    PreScission protease site (GE Healthcare; LEVLFQG…
    Promoter
    T7
    Available Since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    128940-plasmid-map-sequence-id-254597

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  49. pJK057

    Plasmid
    #75036
    Purpose
    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase, N-terminal His6 (H6EcPurE1) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)
    Depositor
    T. J. Kappock, JoAnne Stubbe
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase (purK Escherichia coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes residues 2-7 to His (SSRNNP>HHHHHH)
    Promoter
    T7 and n/a
    Available Since
    Oct. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    75036-plasmid-map-sequence-id-142299

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Showing: 301 - 350 of 22196 results