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Showing: 301 - 350 of 355 results

  9. pGEX-2T-TRX-C751-E3616S

    Plasmid
    #182844
    Purpose
    mutation E3616S (GAA/TCA), PCR product coding C-terminal residues 2976-3726 of TRX was inserted into modified pGEX-2T by Nde I-Nsi I sites. Expression in E. coli. by IPTG induction
    Depositor
    Peter Harte
    Article
    Tie et al Development. 2014 Mar;141(5):1129-39. do
    Insert
    TRX (w Fly)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    182844-plasmid-map-sequence-id-360053

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  10. pcDNA3.1/hFGFR3-K650M-V5

    Plasmid
    #214909
    Purpose
    expression of the K650M mutant variant of human FGFR3, which is associated with Severe Achondroplasia with Developmental Delay and Acanthosis Nigricans (SADDAN)
    Depositor
    Pavel Krejčí
    Article
    Kunova Bosakova et al Proc Natl Acad Sci U S A. 2019 Feb 19. p
    Insert
    FGFR3 (FGFR3 Human)
    Use
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    K650M substitution
    Promoter
    CMV
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214909-plasmid-map-sequence-id-426045

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  11. pCSm5En2CER4(#457)

    Plasmid
    #184090
    Purpose
    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Article
    Rampon et al Development. 2015 May 15;142(10):1840-9.
    Insert
    5myc-chkEn2(C>S)-ERT2
    Use
    Synthetic Biology
    Tags
    5xMyc
    Expression
    Mammalian
    Mutation
    chkEn2: C175S; ERT2: G400V, M543A, L544A, deleted…
    Promoter
    sCMV+SP6
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184090-plasmid-map-sequence-id-365714

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  14. pGGG-Sr43

    Plasmid
    #186974
    Purpose
    Sr43 is a wheat stem rust resistance gene transferred from tall wheat grass (Thinopyrum ponticum) into bread wheat (Triticum aestivum).
    Depositor
    Brande Wulff
    Article
    Yu et al Nat Genet. 2023 Jun;55(6):921-926. doi:
    Insert
    Sr43
    Use
    Tags
    None
    Expression
    Bacterial and Plant
    Mutation
    None
    Promoter
    Natice
    Available since
    April 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    186974-plasmid-map-sequence-id-382711

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  18. pCSmEn2ERT(#117)

    Plasmid
    #184079
    Purpose
    cyclofen-inducible chicken EN2 (wt) activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Article
    Rampon et al Development. 2015 May 15;142(10):1840-9.
    Insert
    6his-myc-chkEn2-ERT2
    Use
    Synthetic Biology
    Tags
    6his-Myc
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available since
    July 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184079-plasmid-map-sequence-id-365956

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  22. pCSmEn2n4ERT(#234)

    Plasmid
    #184082
    Purpose
    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Article
    Rampon et al Development. 2015 May 15;142(10):1840-9.
    Insert
    6his-myc-chkEn2(n4)-ERT2
    Use
    Synthetic Biology
    Tags
    6his-Myc
    Expression
    Mammalian
    Mutation
    chkEn2: G238S; ERT2: G400V, M543A, L544A, deleted…
    Promoter
    sCMV+SP6
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184082-plasmid-map-sequence-id-365718

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  23. pCSmEn2SRERT(#231)

    Plasmid
    #184080
    Purpose
    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Article
    Rampon et al Development. 2015 May 15;142(10):1840-9.
    Insert
    6his-myc-chkEn2(SR)-ERT2
    Use
    Synthetic Biology
    Tags
    6his-Myc
    Expression
    Mammalian
    Mutation
    chkEn2: W247S, F248R; ERT2: G400V, M543A, L544A, …
    Promoter
    sCMV+SP6
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184080-plasmid-map-sequence-id-365710

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  30. pCSmEn25EERT(#232)

    Plasmid
    #184081
    Purpose
    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Article
    Rampon et al Development. 2015 May 15;142(10):1840-9.
    Insert
    6his-myc-chkEn2(5E)-ERT2
    Use
    Synthetic Biology
    Tags
    6his-Myc
    Expression
    Mammalian
    Mutation
    chkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …
    Promoter
    sCMV+SP6
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184081-plasmid-map-sequence-id-363924

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  35. pLZRS-ER-Rac1-Q61L-shortlinker

    Plasmid
    #128584
    Purpose
    For mammalian cell expression of Q61L mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHT
    Depositor
    Julian Downward
    Article
    Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.
    Insert
    Rac1 (Rac1 Mouse)
    Use
    Retroviral
    Tags
    Modified hormone binding domain of ER alpha
    Expression
    Mammalian
    Mutation
    Changed Glutamine61 to Leucine in the RAC1 moiety.
    Promoter
    Available since
    Aug. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    128584-plasmid-map-sequence-id-254045

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  36. pLZRS-ER-Rac1-P29S-shortlinker

    Plasmid
    #128583
    Purpose
    For mammalian cell expression of P29S mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHT
    Depositor
    Julian Downward
    Article
    Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.
    Insert
    Rac1 (Rac1 Mouse)
    Use
    Retroviral
    Tags
    Modified hormone binding domain of ER alpha
    Expression
    Mammalian
    Mutation
    Changed Proline29 to Serine in the RAC1 moiety.
    Promoter
    Available since
    Aug. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    128583-plasmid-map-sequence-id-253120

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  37. pT22Ubm5En2SRE4PCh(#302)

    Plasmid
    #184089
    Purpose
    cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Article
    Rampon et al Development. 2015 May 15;142(10):1840-9.
    Insert
    5myc-chkEn2(SR)-ERT2-P2A-mCherry
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    5xMyc
    Expression
    Mutation
    chkEn2: W247S, F248R; ERT2: G400V, M543A, L544A, …
    Promoter
    Zf-Ubi
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184089-plasmid-map-sequence-id-363975

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  46. pLPE1

    Plasmid
    #209981
    Purpose
    Contains Level 0 Part: E. coli shuttle part (pMB1_ampR_B0017) for the construction of Level 1 plasmids
    Depositor
    Thorsten Mascher
    Article
    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2
    Insert
    E. coli shuttle part (pMB1_ampR_B0017)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    209981-plasmid-map-sequence-id-419436

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  47. pLPE2

    Plasmid
    #209982
    Purpose
    Contains Level 0 Part: E. coli shuttle part (ampR_B0017) for the construction of Level 1 plasmids
    Depositor
    Thorsten Mascher
    Article
    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2
    Insert
    E. coli shuttle part (ampR_B0017)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    209982-plasmid-map-sequence-id-419966

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Showing: 301 - 350 of 355 results