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Plasmid#109786PurposeProtein expression and purification of RBM17_RRMDepositorInsertRBM17_RRM (RBM17 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCruzHA SIRT1
Plasmid#10962DepositorInsertSIRT1 (Sirt1 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166
Plasmid#109328PurposeGateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoterDepositorInsertzCas9
UseCRISPR; Gateway compatible zcas9 entry cloneTags3x FLAG, NLS and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-Lifeact
Plasmid#109545PurposeMultisite gateway vector for 5' tagging with the actin binding motif LifeactDepositorInsertactin binding motif Lifeact
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFLAG/HA-DGCR8
Plasmid#10921DepositorInsertDGCR8 (DGCR8 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro Non-targeting sgRNA 3
Plasmid#109003PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorInsertNon-targeting sgRNA #3
UseLentiviralTagsExpressionMammalianMutationPromotermU6 promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ACTG1_Actin
Plasmid#109989PurposeProtein expression and purification of ACTG1_ActinDepositorInsertACTG1_Actin
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM491-Flag-His
Plasmid#109282PurposeORBIT integrating plasmid for C-terminal tagging with FLAG-His tagDepositorInsertattB-Flag-His
UseOrbit integrating plasmidTagsExpressionMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-ATL2
Plasmid#109020Purposestable lentiviral expression of cDNADepositorInsertATL2 (ATL2 Human)
UseLentiviralTagseGFPExpressionMammalianMutationPromoterEIF-1a promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIDS-LSD1fl
Plasmid#109157PurposeEncodes human full-length LSD1 to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length LSD1, sequence-optimized for insect cells (KDM1A Human)
UseTagsExpressionInsectMutationPromoterp10Available sinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
1162_pAAV-HLP-EmGFP-SpA
Plasmid#109313PurposePlasmid for AAV EmGFP Mammalian ExpressionDepositorInsertEmGFP
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
actc1b-2PH-PLCd-mKate2
Plasmid#109500PurposeMuscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2.DepositorInsertactc1b-2PH-PLCd-mKate2
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmyc-MOV10
Plasmid#10977DepositorInsertMOV10 (MOV10 Human)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLys-M1
Plasmid#109382PurposeArabinose inducible VioB-mCherry expression (stronger RBS). Used to test burden response in E. coliDepositorInsertpARABAD-VioB-mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-CoRESTfl
Plasmid#109156PurposeEncodes human full-length CoREST with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length CoREST, sequence-optimized for insect cells (RCOR1 Human)
UseTags3xFLAGExpressionInsectMutationPromoterPolyhedrinAvailable sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTA-Luc SIRT1 promoter (-202)
Plasmid#10971DepositorInsertSIRT1 promoter (Sirt1 Mouse)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Rod Opsin
Plasmid#109361PurposeHuman rod opsinDepositorInsertRod opsin (RHO Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pHH0103_ESRRA_Hormone-recep
Plasmid#109908PurposeProtein expression and purification of ESRRA_Hormone-recepDepositorInsertESRRA_Hormone-recep (ESRRA Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME-mKate2-NS
Plasmid#109729PurposeMultisite gateway vector for 5' tagging with mKate2DepositorInsertmKate2
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only