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pCIneo-NHA

Plasmid

#115359

Purpose

Cloning vector to produce NHA-fusion proteins without any other gene insert

Depositor

Witold Filipowicz, Ramesh Pillai

Article

Pillai et al RNA. 2004 Oct . 10(10):1518-25.

Insert

N peptide and HA

Use

Tags

N peptide and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCambia1300-PacI

Plasmid

#44183

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 14, 2013

Availability

Academic Institutions and Nonprofits only

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pET-AqpZ-ALFA

Plasmid

#212310

Purpose

Expression of AqpZ with ALFA tag

Depositor

Arthur Laganowsky

Article

Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

Insert

Aquaporin Z

Use

Tags

ALFA tag and Strep-Tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pTNT-pT7-tetR-mCherry

Plasmid

#140870

Purpose

T7 promoter driven synthesis of TetR-mCherry fusion protein

Depositor

Neal Devaraj

Article

Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

Insert

TetR-mCherry expressed with T7 promoter

Use

Synthetic Biology

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pClodANL-FGF2YE

Plasmid

#107872

Purpose

Bacterial expression of FGF2phosphomimic-NanoLuc

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Insert

FGF2-phosphomimic

Use

Luciferase

Tags

NanoLuc

Expression

Bacterial

Mutation

Y82E

Promoter

pro D

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pRRL-MHCK7-GCaMP6

Plasmid

#65042

Purpose

Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7

Depositor

Nenad Bursac

Article

Madden et al eLife. 2015 Jan 9;4:e04885. doi: 10.7554

Insert

GCaMP6

Use

Lentiviral

Tags

6xHis, T7 tag, and Xpress epitope

Expression

Mammalian

Mutation

Promoter

MHCK7

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pET29-bdSUMO-Anti-ALFA nanobody

Plasmid

#212311

Purpose

Expression of anti-ALFA nanobody

Depositor

Arthur Laganowsky

Article

Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

Insert

anti-ALFA nanobody

Use

Tags

bdSUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pQE80L SpyTag-ELP-SpyTag

Plasmid

#112634

Purpose

Two SpyTags linked by an elastin-like polypeptide for hydrogel formation

Depositor

Mark Howarth

Article

Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

Insert

SpyTag-ELP-SpyTag

Use

Tags

His6

Expression

Bacterial

Mutation

central cysteine

Promoter

T5

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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CellTagA

Plasmid

#124591

Purpose

Expressed barcode for sample multiplexing in single-cell analysis

Depositor

Samantha Morris

Article

Guo et al Genome Biol. 2019 May 9;20(1):90. doi: 1

Insert

CellTagA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pSB819-PKR-hum

Plasmid

#20030

Depositor

Harmit Malik

Article

Elde et al Nature. 2008 Nov 30. ():.

Insert

Protein kinase R (EIF2AK2 Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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CellTagB

Plasmid

#124592

Purpose

Expressed barcode for sample multiplexing in single-cell analysis

Depositor

Samantha Morris

Article

Guo et al Genome Biol. 2019 May 9;20(1):90. doi: 1

Insert

CellTagB

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pFUdioFRTeYFPW

Plasmid

#133998

Purpose

expresses eYFP upon coexpression with the recombinase flippase (Flp)

Depositor

Elly Nedivi

Article

Subramanian et al Cell Rep. 2019 Aug 6;28(6):1584-1595.e5.

Insert

eYFP

Use

Lentiviral; Flp/frt

Tags

Expression

Mammalian

Mutation

inverted and flanked by two incompatible FRT site…

Promoter

UbC

Available since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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pRC319

Plasmid

#61272

Purpose

Also referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.

Depositor

Timothy Lu

Article

Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

Insert

Cas9, tracrRNA, crRNA

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pQE80L TriCatcher-ELP

Plasmid

#112630

Purpose

Two SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcher

Depositor

Mark Howarth

Article

Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

Insert

TriCatcher-ELP

Use

Tags

His6 and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T5

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pTNT-pT7-tetR-sfGFP

Plasmid

#140868

Purpose

T7 promoter driven synthesis of C-terminal fusion of sfGFP to TetR repressor (TetR-sfGFP)

Depositor

Neal Devaraj

Article

Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

Insert

TetR-sfGFP expressed with T7 promoter

Use

Synthetic Biology

Tags

sfGFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pET-MhCINS-6xHis

Plasmid

#194304

Purpose

Bacterial expression of MhCINS protein with a C terminal His-tag and transit peptide removed at N terminal

Depositor

Richard Saunders

Article

Liu et al J Ecology 2024; 00, 1–20. doi: 10.1111/1

Insert

MhCINS

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pGW1-ExSYTE-P2A-EGFP

Plasmid

#216257

Purpose

ExSYTE (P2A) EGFP

Depositor

Susumu Tomita

Article

Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

Insert

ExSYTE P2A EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGW1-ExSYTE-EGFP

Plasmid

#216259

Purpose

ExSYTE - EGFP fusion

Depositor

Susumu Tomita

Article

Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

Insert

ExSYTE EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGEM-HE-ExSYTE

Plasmid

#216265

Purpose

ExSYTE in oocyte vector

Depositor

Susumu Tomita

Article

Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

Insert

ExSYTE

Use

Crna synthesis for xenopus laevis oocyte

Tags

none

Expression

Mutation

Promoter

Available since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK2 RPS28 UTR1 WT

Plasmid

#203157

Purpose

RPS28 UTR luciferase assay plasmid (WT UTR1, sites 1 and 2)

Depositor

Susan Baserga

Article

Bryant et al Nucleic Acids Res. 2024 Feb 28;52(4):198

Insert

RPS28 UTR (RPS28 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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