We narrowed to 12,076 results for: ache
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Plasmid#50955Purposeflexed-ReaChR-citrine in AAV2 vector under human synapsin promoterDepositorInsertReaChR-citrine
UseAAVTagscitrineExpressionMutationPromoterhuman synapsin promoterAvailable sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-PuroR_BACH2-sgRNA8
Plasmid#71828PurposeExpression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a controlDepositorInsertBACH2-sgRNA8 (BACH2 S. pyogenes, Human, Synthetic)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterU6Available sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mNaChBacMut-T2A-tdTomato
Plasmid#60657PurposeExpresses mNaChBacMut-T2A-tdTomatoDepositorInsertmNaChBacMut-T2A-tdTomato
UseTagsExpressionMammalianMutationE191KPromoterAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFastbacHTB HA-human PP2A Cα D88N
Plasmid#132633Purposeexpresses HA-human PP2A Cα D88N in Hi5 insect cellDepositorInsertPP2A Cα D88N
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG-FLEX.iAChSnFR-Venus-NULL
Plasmid#137960PurposeExpresses FLEXed iAChSnFR (non-binding yellow version) under CAG promoterDepositorArticleInsertiAChSnFR (non-binding yellow variant)
UseAAV and Cre/LoxTagsExpressionMammalianMutationY140A binding site mutationPromoterCAGAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[Spinach2]
Plasmid#68430PurposeTransient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing the "Spinach2" aptamer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-AMV-α1L9'S-nAchR
Plasmid#20684DepositorInsertnicotinic acetylcholine receptors (Chrna1 Mouse)
UseTagsAMV and pA 50ExpressionBacterialMutationLeucine at 9' position in M2 region changed …PromoterAvailable sinceApril 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-PKA-RIIβ-sREACh
Plasmid#114113PurposeMammalian expression of rat PKA regulatory subunit IIβ C-terminally tagged by sREACh.DepositorInsertPKA regulatory subunit RII beta tagged by sREACh (low-irradiant YFP)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
alpha7-nAChR(scramble control)
Plasmid#86649Purposeexpression of control shRNADepositorInsertCHRNA7 (control)
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS-AMV-δ1L9'S-nAchR
Plasmid#20687DepositorInsertnicotinic acetylcholine receptors
UseTagsAMV and pA 50ExpressionBacterialMutationLeucine at 9' position in M2 region changed …PromoterAvailable sinceApril 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6-YFP
Plasmid#50483Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
UseTagsYFP fusion in M3-M4 loop (after residue A405)ExpressionMammalianMutationGly-Ala-Gly flexible linker flanking the YFP open…PromoterCMVAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS-AMV-β1L9'S-nAchR
Plasmid#20685DepositorInsertnicotinic acetylcholine receptors (Chrnb1 Mouse)
UseTagsAMV and pA 50ExpressionBacterialMutationLeucine at 9' position in M2 region changed …PromoterAvailable sinceApril 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
LLP338 pEF1a-DACH1-1-SunTag
Plasmid#100942PurposeTALE vector with DACH1-1 insert fused to SunTagx10, driven by EF1a, with 3xHA tag, no PURO geneDepositorInsertTALE target sequence for DACH1, SunTag array (10xGCN4)
UseTALENTags3xHAExpressionMammalianMutationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-nAChr-beta3-CFP
Plasmid#50485Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
UseTagsCFPExpressionMammalianMutationCFP fusion in M3-M4 loop (after residue P379). O…PromoterCMVAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS-AMV-γ1L9'S-nAchR
Plasmid#20686DepositorInsertnicotinic acetylcholine receptors (Chrng Mouse)
UseTagsAMV and pA 50ExpressionBacterialMutationLeucine at 9' position in M2 region changed …PromoterAvailable sinceApril 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
Ec holA-pET(11a)(ACH-)
Plasmid#237233Purposeover-express E. coli holA (Pol III delta subunit)DepositorInsertholA (delta) (holA E. coli)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ec holB-pET(11a)(ACH-)
Plasmid#237234Purposeover-express E. coli holB (Pol III delta prime subunit)DepositorInsertholB (delta prime) (holB E. coli)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FFSS-BACH1 C122E
Plasmid#232274PurposeExpression of N-terminal tagged 2xFLAG-2xSTREP-BACH1 (H. sapiens) with Cys122 to Glu point mutation in human cell linesDepositorInsertBACH1 (BACH1 Human)
UseTags2xFLAG-2xSTREPExpressionMammalianMutationchanged cysteine 122 to glutamic acidPromoterCMVAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
gWiz_BM40-vAchT-Cytosolic-VL-hIgG1-CL
Plasmid#218447Purposemammalian expression plasmid for the light chain fragment of an anti-vAchT Fab domain binding to the cytosolic side of the transporterDepositorInsertvAchT-Cytosolic-VL-hIgG1-CL
UseAffinity Reagent/ AntibodyTagsBM40ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-VAChT [N425/45R-1]
Plasmid#211600PurposeMammalian Expression Plasmid of anti-VAChT (Rat) subclass-switched IgG1 R-mAb. Derived from hybridoma N425/45.DepositorInsertanti-VAChT (Rattus norvegicus) recombinant mouse monoclonal antibody. (Slc18a3 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceJan. 4, 2024AvailabilityAcademic Institutions and Nonprofits only