We narrowed to 7,412 results for: CIT

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pHH0103_AC104650.1-1/1

Plasmid

#91541

Purpose

Protein expression and purification of human SH3 domain construct AC104650.1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

AC104650.1-1/1

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_AC093799.1-1/1

Plasmid

#91495

Purpose

Protein expression and purification of human SH3 domain construct AC093799.1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

AC093799.1-1/1

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_OTOR-2/2

Plasmid

#91382

Purpose

Protein expression and purification of human SH3 domain construct OTOR-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

OTOR-2/2

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_OTOR-1/2

Plasmid

#91328

Purpose

Protein expression and purification of human SH3 domain construct OTOR-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

OTOR-1/2

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_RIMBP3B-1/1

Plasmid

#91299

Purpose

Protein expression and purification of human SH3 domain construct RIMBP3B-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

RIMBP3B-1/1

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_LOC440461-1/1

Plasmid

#91309

Purpose

Protein expression and purification of human SH3 domain construct LOC440461-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

LOC440461-1/1

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_C1orf168-1/1

Plasmid

#91295

Purpose

Protein expression and purification of human SH3 domain construct C1orf168-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

C1orf168-1/1

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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LLhG-pHG165a

Plasmid

#90039

Purpose

Constitutive expression of a chimeric LacI:GalR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Proteins. 2008 Dec;73(4):941-57. doi: 10

Insert

LLhG

Expression

Bacterial

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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TU#1412_tiam-1_cDNA

Plasmid

#82364

Purpose

express tiam-1 cDNA

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

tiam-1 cDNA (tiam-1 Nematode)

Expression

Worm

Promoter

none

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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TU#1449_unc-73e_cDNA

Plasmid

#82363

Purpose

express unc-73e cDNA

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

unc-73 e isoform (unc-73 Nematode)

Expression

Worm

Promoter

none

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only
pPB-CAG-zNanogHD-pgk-hph

Plasmid

#74910

Purpose

Mammalian expression of zNanog Homeodomain

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pJK359

Plasmid

#71570

Purpose

Produces Acetobacter aceti 1023 putative acyl-CoA:carboxylate CoA-transferase (UctA)

Depositor

T. J. Kappock

Article

Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

Insert

putative CoA-transferase

Expression

Bacterial

Promoter

T7

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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FCIV1-GR SS287AA

Plasmid

#72703

Purpose

Lentiviral expression of GR Phospho-deficient mutant (2nd generation)

Depositor

Freddy Jeanneteau

Article

Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

Insert

GR (Nr3c1 Rat)

Use

Lentiviral

Tags

IRES-Venus

Expression

Mammalian

Mutation

SS287AA

Promoter

ubiquitin

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
pSTARR-seq_fly-x16

Plasmid

#71505

Purpose

flySTARR-seq screening vector with x16 core promoter

Depositor

Alexander Stark

Article

Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Available Since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pJK286

Plasmid

#71567

Purpose

Produces Acetobacter aceti 1023 formyl-CoA:oxalate CoA-transferase (UctB)

Depositor

T. J. Kappock

Article

Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

Insert

formyl-CoA:oxalate CoA-transferase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJK358

Plasmid

#71569

Purpose

Produces Acetobacter aceti 1023 putative acyl-CoA:carboxylate CoA-transferase (UctD)

Depositor

T. J. Kappock

Article

Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

Insert

putative CoA-transferase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-RpS12-Gateway

Plasmid

#71508

Purpose

flySTARR-seq validation vector with Gateway cassette downstream of the firefly luciferase gene and the RpS12 core promoter

Depositor

Alexander Stark

Article

Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

Type

Empty backbone

Use

Flystarr-seq validation vector

Tags

luciferase

Expression

Insect

Available Since

Dec. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pSTARR-seq_fly-eEF1delta

Plasmid

#71501

Purpose

flySTARR-seq screening vector with eEF1delta core promoter

Depositor

Alexander Stark

Article

Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pES004 (pTet-qacR 5)

Plasmid

#69033

Purpose

qacR 5 downstream of a Tet promoter

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Insert

qacR (qacR Synthetic)

Tags

His6 tag

Expression

Bacterial

Mutation

W61Y, E90Q, F102S, M116Q, Y119L, E120Q

Promoter

pTet

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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AIRAP 5: AIRAP(1-50).GST,V1.pcDNA3

Plasmid

#21795

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

AIRAP (Zfand2a Mouse)

Tags

GST

Expression

Mammalian

Mutation

amino acids 1-50

Available Since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal-RNQ1 L94A-YFP

Plasmid

#18692

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 L94A (RNQ1 Budding Yeast)

Tags

YFP

Expression

Yeast

Mutation

Leucine 94 mutated to Alanine (L94A)

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
Sup35 Sc/Ca NM Chimera 4 G/A His7 Cys

Plasmid

#15602

Depositor

Susan Lindquist

Article

Tessier et al Nature. 2007 May 31. 447(7144):556-61.

Insert

Sup35 NM (SUP35 Candida albicans, Budding Yeast)

Tags

7xHis and Cysteine

Expression

Bacterial

Mutation

4 G/A. Four glycines mutated to alanines. The pos…

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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FH95pUP95S73DGW (F3)

Plasmid

#74040

Purpose

lentiviral expression of Psd95 shRNA and Psd95-S73D-EGFP fusion

Depositor

Robert Malenka, Bernardo Sabatini, Weifeng Xu

Article

Steiner et al Neuron. 2008 Dec 10;60(5):788-802. doi:

Insert

Psd95

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

S73D

Promoter

H1

Availability

Academic Institutions and Nonprofits only
BA31_pU.CAG.SaCas9-D10A.rBGpA

Plasmid

#199251

Purpose

Expression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Insert

SaCas9.D10A nickase

Use

CRISPR

Tags

3XHA epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

D10A

Promoter

CAG promoter

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-iCas9-neo

Plasmid

#85400

Purpose

Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence

Depositor

Qin Yan

Article

Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

Insert

Flag-iCas9-P2A-GFP

Use

Lentiviral

Tags

Flag-iCas9-P2A-GFP

Available Since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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BPK1520

Plasmid

#65777

Purpose

Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

SpCas9 gRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_SV40_Large_T_Antigen-P2A-Hygro_Barcode

Plasmid

#170255

Purpose

Barcoded lentiviral vector to express SV40 Large T Antigen in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

SV40 Large T Antigen

Use

Lentiviral

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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4XCLEAR-luciferase reporter

Plasmid

#66800

Purpose

Reporter for activity of the CLEAR network

Depositor

Albert La Spada

Article

Cortes et al Nat Neurosci. 2014 Sep;17(9):1180-9. doi

Insert

4XCLEAR

Use

Luciferase

Promoter

Four CLEAR elements (GTCACGTGAC) in tandem derive…

Available Since

Nov. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pFudio-Cpg15-IRES2-TdTomatoW

Plasmid

#134309

Purpose

Expresses cpg15 and TdTomato upon coexpression with Cre recombinase

Depositor

Elly Nedivi

Article

Subramanian et al Cell Rep. 2019 Aug 6;28(6):1584-1595.e5.

Inserts

cpg15 (Nrn1 Mouse, Rat)
TdTomato

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

UbC

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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beta-arrestin1 mYFP

Plasmid

#36916

Depositor

Robert Lefkowitz

Article

Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

Insert

beta-arrestin1 (Arrb1 Rat)

Tags

mYFP

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2012

Availability

Academic Institutions and Nonprofits only

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