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HA-sav

Plasmid

#32834

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

Sav (SAV1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
LentiCRISPRv2-Opti

Plasmid

#163126

Purpose

LentiCRISPRv2 variant with modified sgRNA scaffold from Chen et al. 2013 Cell.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-PGK-PYL1-sfGFP-Coilin

Plasmid

#122177

Purpose

expression of PYL1-SfGFP-Coilin, lentivirus vector

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

Coilin (COIL Human)

Use

Lentiviral

Tags

PYL1-SFGFP

Expression

Mammalian

Promoter

PGK

Available Since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-OTUB1 WT-pcDNA3.1

Plasmid

#118209

Purpose

Expresses FLAG-OTUB1 WT in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

OTUB1 (OTUB1 Human)

Tags

3x FLAG

Expression

Mammalian

Mutation

none

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAN-PTet-dCas9

Plasmid

#62244

Purpose

controls the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

dCas9

Use

CRISPR

Expression

Bacterial

Mutation

catalytically inactive, D10A, H840A

Promoter

Ptet

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pCBC-MT2T3

Plasmid

#50594

Purpose

CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Insert

T2, gRNA scaffold, TaU3t plus, U6-26p, T3

Use

CRISPR; Pcr template

Expression

Plant

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pMRXIP Turquoise2-Syntaxin17

Plasmid

#89938

Purpose

Expresses Syntaxin17 tagged with Turquoise2 in mammalian cells

Depositor

Noboru Mizushima

Article

Tsuboyama et al Science. 2016 Nov 25;354(6315):1036-1041

Insert

human Syntaxin 17 (STX17 Human)

Use

Retroviral

Tags

mTurquoise2

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pKIR1.0

Plasmid

#86409

Purpose

CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

Depositor

Tetsuya Higashiyama

Article

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG and NLS

Promoter

AtRPS5A

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pERB221

Plasmid

#61502

Purpose

const. CenpB-GFP-Halo + mCherry-DHFR

Depositor

David Chenoweth, Michael Lampson

Article

Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

Inserts

CenpB-GFP-Halo
mCherry-DHFR

Tags

HaloTag and mCherry

Expression

Mammalian

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human cofilin S3E

Plasmid

#50855

Purpose

expresses psuedo-phosporylated, non-activatable cofilin in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3E (CFL1 Human)

Expression

Mammalian

Mutation

S3E psuedo-phosporylated, non-activatable

Promoter

CMV

Available Since

Feb. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
TRE.IkBSR-Flag Pgk.Cre

Plasmid

#22504

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

IkBSR

Use

Cre/Lox and Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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Tom20-CIB-GFP

Plasmid

#117242

Purpose

Light activated recruitment to OMM in mammalian cells with Cry2/CIB optogenetic switch

Depositor

Robert Hughes

Article

Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

Insert

CIBN

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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PCMV-intron myc Rab11 S25N

Plasmid

#46786

Purpose

expression of S25N Rab11a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

RAB11A S25N (RAB11A Human)

Tags

myc

Expression

Mammalian

Mutation

S25N dominant negative

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pET28CLY3

Plasmid

#21762

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 3 SGGSGG repeats

Tags

His

Expression

Bacterial

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pSCT-GAL93-SFPQ

Plasmid

#46320

Depositor

Steven Brown

Article

Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

Insert

SFPQ (SFPQ Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only
Teto-FUW-Pax6

Plasmid

#61541

Purpose

Expresses Pax6 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Pax6 (Pax6 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pBUN6A11

Plasmid

#50579

Purpose

CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Inserts

dCas9-VP64
gRNA scaffold

Use

CRISPR; Plant expression

Tags

3xFLAG, HA, and NLS

Mutation

dCas9 = defective in DNA cleavage; 4×minimal VP16…

Promoter

OsU3p and Ubi1p

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pFB2B

Plasmid

#195284

Purpose

Bicistronic HSV-TK and Puromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A Puro

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC-hTRF1

Plasmid

#64164

Purpose

Retroviral vector expressing human TRF1 with N-terminal MYC tag

Depositor

Titia de Lange

Article

Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a His6HuR

Plasmid

#135943

Purpose

Expresses HuR in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

ELAV like RNA binding protein 1 (ELAVL1 Human)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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