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pU6-crRNA(TBK1)

Plasmid

#109322

Purpose

AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TBK1

Depositor

Feng Zhang

Article

Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

Insert

mCherry-KASH (TBK1 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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sgNF2

Plasmid

#229433

Purpose

knockout of NF2

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgNF2 (NF2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6;bU6;EFS

Available since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2b_(CJT93)

Plasmid

#226992

Purpose

pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2b must be used with gRNA 1a or 1b

Depositor

Benjamin Kleinstiver

Article

Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

Insert

SpCas9 gRNA 2b to create OPA1-del_exon5

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(PDLIM1_5-1)-PGKpuroBFP-W

Plasmid

#211978

Purpose

Express gRNA against PDLIM1 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting PDLIM1 (PDLIM1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-ENO1_sgRNA1

Plasmid

#201592

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

ENO1 (ENO1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 LIPT2-2

Plasmid

#184483

Purpose

Lentivirus gRNA targeting human LIPT2 gene

Depositor

Todd Golub, Peter Tsvetkov

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

LIPT2 (LIPT2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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IP6K3 gRNA (BRDN0001147058)

Plasmid

#77654

Purpose

3rd generation lentiviral gRNA plasmid targeting human IP6K3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

IP6K3 (IP6K3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ADCK1 gRNA (BRDN0001149367)

Plasmid

#76087

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADCK1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADCK1 (ADCK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ADK gRNA (BRDN0001146410)

Plasmid

#75743

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADK (ADK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FXN gRNA (BRDN0001144784)

Plasmid

#77813

Purpose

3rd generation lentiviral gRNA plasmid targeting human FXN

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FXN (FXN Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pX330-FOXL2-cterm

Plasmid

#192893

Purpose

Expresses Cas9 and sgRNA targeting the C-terminus region of FOXL2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

FOXL2 C-term sgRNA (FOXL2 Synthetic)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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plentiCRISPRv2-PURO-LYSET-gRNA2

Plasmid

#210490

Purpose

CRISPR pooled KO of human LYSET

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-gRNA2 (LYSET Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2 puro - CRBN (#2)

Plasmid

#166241

Purpose

sgRNA (#2) to generate a knockout of human CRBN by targeting the start region of Exon1

Depositor

Günter Schneider

Article

Lier et al Bioorg Chem. 2021 Nov 20:105505. doi: 10

Insert

CRBN (CRBN Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pXPR_003-sgKCMF1

Plasmid

#202441

Purpose

Expression of a guide RNA targeting KCMF1

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

KCMF1 gRNA (KCMF1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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CDK15 gRNA (BRDN0001145059)

Plasmid

#76483

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK15

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK15 (CDK15 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NEK1 gRNA (BRDN0001146251)

Plasmid

#76125

Purpose

3rd generation lentiviral gRNA plasmid targeting human NEK1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NEK1 (NEK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP2K1 gRNA (BRDN0001147078)

Plasmid

#76211

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K1 (MAP2K1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP2K2 gRNA (BRDN0001147805)

Plasmid

#77600

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K2 (MAP2K2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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KIT gRNA (BRDN0001147662)

Plasmid

#77562

Purpose

3rd generation lentiviral gRNA plasmid targeting human KIT

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

KIT (KIT Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ALDH18A1 gRNA (BRDN0001145444)

Plasmid

#77995

Purpose

3rd generation lentiviral gRNA plasmid targeting human ALDH18A1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ALDH18A1 (ALDH18A1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge