We narrowed to 4,032 results for: pCMV mammalian

1. pCMV6-AN-HA_aa 1-420 POLI

   Plasmid

   #201582

   Purpose

   Expresses the catalytic domain (aa 1-420) of human DNA polymerase iota with a HA tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   DNA polymerase iota (POLI Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Expresses amino acids 1-420 of DNA polymerase iota

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. hSLC17A6/vGlutT2/pCMV6-entry

   Plasmid

   #196939

   Purpose

   Plasmid expressing an antigen targeting the VGluT2 transporter

   Depositor

   James Trimmer

   Insert

   vGlutT2/ SLC17A6
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV_SS-HA-V5-LaccID-CD4 TM_pDisplay

   Plasmid

   #234046

   Purpose

   expresses LaccID on the mammalian cell surface

   Depositor

   Alice Ting

   Article

   Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

   Insert

   LaccID-CD4TM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3.1-pCMV-PfV-GS-Sapphire

   Plasmid

   #116933

   Purpose

   Mammalian vector for expression of the 40nm GEM

   Depositor

   Liam Holt

   Article

   Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

   Insert

   Pfv
   

   Use

   Tags

   Sapphire

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-hA3A-eBE-Y130F

   Plasmid

   #113423

   Purpose

   Expresses hA3A-eBE-Y130F in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3A-eBE-Y130F (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   hAPOBEC3A_Y130F

   Promoter

   CMV

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-SARS-CoV-2-Nsp3PLpro

   Plasmid

   #204208

   Purpose

   Mammalian expression of SARS-CoV-2 Nsp3

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
   

   Use

   Tags

   FLAGx1

   Expression

   Mammalian

   Mutation

   PLpro of Nsp3

   Promoter

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-mTagBFP2-FRB-Rab7

   Plasmid

   #214273

   Purpose

   Mammalian expression of mTagBFP2-FRB-Rab7 (late endosome marker)

   Depositor

   Shinya Tsukiji

   Article

   Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

   Insert

   mTagBFP2-FRB-Rab7A (RAB7A Canis lupus familiaris (Dog))
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-SARS-CoV-2 Nsp3TMC111A

   Plasmid

   #204207

   Purpose

   Mammalian expression of SARS-CoV-2 Nsp3

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
   

   Use

   Tags

   FLAGx1

   Expression

   Mammalian

   Mutation

   Cys to Ala in active site of PLpro

   Promoter

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-SARS-CoV-2 Nsp3TM

   Plasmid

   #204206

   Purpose

   Mammalian expression of SARS-CoV-2 Nsp3

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
   

   Use

   Tags

   FLAGx1

   Expression

   Mammalian

   Mutation

   none, from obtained clone

   Promoter

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcmv3-STING-C88S-FLAG

    Plasmid

    #198187

    Purpose

    Mammalian expression of FLAG-tagged mouse STING1 C88S

    Depositor

    Yun Zhao

    Article

    Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

    Insert

    TMEM173 (Sting1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV 3xFLAG-ATG3 C264A

    Plasmid

    #129297

    Purpose

    Expresses 3xFLAG-ATG3 C264A in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Cysteine 264 to Alanine

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-myc3-Mdm2 D367A

    Plasmid

    #52058

    Purpose

    expression of non-cleavable Mdm2 in mammalian cells

    Depositor

    Trudy Oliver, Tyler Jacks

    Article

    Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

    Insert

    Mdm2 D367A (MDM2 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    D367A

    Promoter

    CMV

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pCMV-myc3-Mdm2 p60

    Plasmid

    #52059

    Purpose

    expression of truncated Mdm2 in mammalian cells

    Depositor

    Trudy Oliver, Tyler Jacks

    Article

    Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

    Insert

    Mdm2 p60 (truncated) (MDM2 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    p60 (truncated at D367, DVDP motif)

    Promoter

    CMV

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pCMV-hA3A-eBE-Y132D

    Plasmid

    #113429

    Purpose

    Expresses hA3A-eBE-Y132D in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3A-eBE-Y132D (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    hAPOBEC3A_Y132D

    Promoter

    CMV

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-myc3-Mdm2 D367E

    Plasmid

    #52057

    Purpose

    expression of non-cleavable Mdm2 in mammalian cells

    Depositor

    Trudy Oliver, Tyler Jacks

    Article

    Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

    Insert

    Mdm2 D367E (MDM2 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    D367E

    Promoter

    CMV

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pCMV 3xFLAG-ATG3 K243R

    Plasmid

    #129301

    Purpose

    Expresses pCMV 3xFLAG-ATG3 K243R in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Lysine 243 to Arginine

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-ABEMAX-NLS-P2A_BLAST

    Plasmid

    #135355

    Purpose

    Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    ABEMax-2XNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV5D HA-PPM1H S124A/S211A

    Plasmid

    #208370

    Purpose

    Mammalian expression of HA-tagged human PPM1H S124A/S211A

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    HA-PPM1H S124A/S211A (PPM1H Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S124A/S211A

    Promoter

    Available since

    Dec. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV6-AN-HA_aa 1-542 POLK

    Plasmid

    #201585

    Purpose

    Expresses the N-terminal clasp domain and catalytic domain (aa 1-542) of human DNA polymerase kappa with a HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    Polymerase kappa (POLK Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Expresses amino acids 1-542 of DNA polymerase kap…

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV6-AN-DDK_RKK/AAA POLI

    Plasmid

    #131240

    Purpose

    Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations R524A, K526A and K530A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains point mutations that convert arginine re…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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