178,651 results
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Raptor_2 shRNA
Plasmid#1858DepositorAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE
Plasmid#138150PurposeFor cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInserthU6_Rfxcas13d-DR1_BsmBI
UseLentiviralMutationDR1 A>TAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP
Plasmid#102796PurposeRetrovirus for delivery of one sgRNA (empty, bbsI)DepositorInsertU6 sgRNA
UseCRISPR and RetroviralExpressionMammalianPromoterU6Available sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
miniTurbo-His6_pET21a
Plasmid#107178Purposeexpresses His6-tagged miniTurbo in bacteriaDepositorInsertminiTurbo (BirA mutant)
ExpressionBacterialMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Available sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lck-mScarlet-I
Plasmid#98821PurposeIn vivo visualization of the plasma membrane (can be used for colocalization studies)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLck
TagsmScarlet-IExpressionMammalianPromoterCMVAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMK293 (mAID-mCherry2-Hygro)
Plasmid#72831PurposeC-terminal tagging with mAID-mCherry2 using CRISPR/Cas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmAID-mCherry2-Hygro
UseCRISPRTagsmAID-mCherry2ExpressionMammalianAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28-MBP-TEV
Plasmid#69929Purposebacterial expression of MBP-TEV n-terminal fusionsDepositorPublicationTypeEmpty backboneTagsMBP and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available sinceJan. 8, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV-LUC2CP/ARE
Plasmid#62857PurposeSplicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLuciferase
UseLuciferaseExpressionMammalianMutationdestabilizing sequences (DS) added to the C termi…PromoterCMVAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330S-2
Plasmid#58778PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FH-Tet2-pEF
Plasmid#41710PurposeThis plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian systemDepositorInserttet methylcytosine dioxygenase 2 (Tet2 Mouse)
TagsFLAG and HAExpressionMammalianPromoterEF1aAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-MCS2
Plasmid#46954PurposeCloning plasmid for the generation of Knock-In in human cells using rAAV.DepositorPublicationTypeEmpty backboneUseAAVAvailable sinceSept. 19, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV-R-GECO1.2
Plasmid#45494PurposeRed intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertR-GECO1.2
ExpressionMammalianMutationR-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…PromoterCMVAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR1A-Huwe1
Plasmid#37431DepositorInsertHuwe1 (HUWE1 Human)
UseGateway CloningAvailable sinceSept. 25, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-CMV-jGCaMP8m
Plasmid#162372PurposeMammalian expression of ultrafast protein calcium sensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertjGCaMP8m
Tags6xHisExpressionMammalianMutationA25G F286YPromoterCMVAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pACRISPR
Plasmid#113348PurposeA sgRNA expression plasmid for genome editing in Pseudomonas aeruginosaDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lambda Phosphatase
Plasmid#79748PurposeIntended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.DepositorInsertLambda Phosphatase
ExpressionBacterialPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pULTRA-CNF
Plasmid#48215Purposehighly efficient suppressor plasmidDepositorInsertspolyspecific MJ tyrosyl synthetase
tRNA
ExpressionBacterialMutationY32L, L65V, F108W, Q109M, D158G, I159APromoterproK and tacIAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCI-neo-mOVA
Plasmid#25099DepositorInsertmembrane bound ovalbumin
ExpressionBacterial and MammalianAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-myc3-CUL4B
Plasmid#19922DepositorPublicationAvailable sinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by
