We narrowed to 2,433 results for: PRK

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pHH0103_MAP3K9-1/2

Plasmid

#91313

Purpose

Protein expression and purification of human SH3 domain construct MAP3K9-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MAP3K9-1/2 (MAP3K9 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARF4-GFP

Plasmid

#67392

Purpose

Mammalian expression of hArf4 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF4 (ARF4 Human)

Tags

GFP

Expression

Mammalian

Mutation

V53A, L123P and M134I mutations were unintended P…

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pPN186

Plasmid

#91573

Purpose

Express sgRNA targeting human AKT3

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

AKT3 (AKT3 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN187

Plasmid

#91574

Purpose

Express sgRNA targeting human AKT3

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

AKT3 (AKT3 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-HA-MEK1 C121S

Plasmid

#53164

Purpose

Expresses GST-HA tagged human MEK1 C121S from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Tags

GST and HA

Expression

Bacterial

Mutation

C121S

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pSUPER-retro-puro-tetO-Mek1-shRNA

Plasmid

#53179

Purpose

Expresses shRNA against mouse Mek1 from puromycin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (Map2k1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

June 19, 2014

Availability

Academic Institutions and Nonprofits only
His 3C Kinase dead MKK6 1-334

Plasmid

#87882

Purpose

E. coli expression of MKK6 kinase dead

Depositor

Peter Mace

Article

Weijman et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

MKK6 (MAP2K6 Human)

Tags

3C protease site and 6xHis

Expression

Bacterial

Mutation

K82A, kinase dead mutant

Promoter

T7

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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WNK1 gRNA (BRDN0001146391)

Plasmid

#75622

Purpose

3rd generation lentiviral gRNA plasmid targeting human WNK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

WNK1 (WNK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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WNK1 gRNA (BRDN0001147437)

Plasmid

#75623

Purpose

3rd generation lentiviral gRNA plasmid targeting human WNK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

WNK1 (WNK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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WNK1 gRNA (BRDN0001148217)

Plasmid

#75624

Purpose

3rd generation lentiviral gRNA plasmid targeting human WNK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

WNK1 (WNK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-HA-MEK1 CBM

Plasmid

#53163

Purpose

Expresses GST-HA tagged human MEK1 M187AH188AM230AH239A from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Tags

GST and HA

Expression

Bacterial

Mutation

M187A/H188A/M230A/H239A

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pGEX6P1-HA-MEK5 CBM-E

Plasmid

#53168

Purpose

Expresses GST-HA tagged human MEK5 L280AH281AM323AQ332A from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK5 (MAP2K5 Human)

Tags

GST and HA

Expression

Bacterial

Mutation

L280A/H281A/M323A/Q332A

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pJL394

Plasmid

#243718

Purpose

Stably expresses a Myc-tagged AKAP1 with tudor domain deleted and gRNA-resistant silent mutations

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

AKAP1 (AKAP1 Human)

Use

Lentiviral

Tags

Myc

Mutation

tudor domain deleted and gRNA-resistant silent mu…

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pJL396

Plasmid

#243719

Purpose

Stably expresses a Myc-tagged AKAP1 with PKA helix deleted and gRNA-resistant silent mutations

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

AKAP1 (AKAP1 Human)

Use

Lentiviral

Tags

Myc

Mutation

PKA helix deleted and gRNA-resistant silent mutat…

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pJL349

Plasmid

#243708

Purpose

Stably expresses a Myc-tagged wild-type AKAP1 containing gRNA-resistant silent mutations

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

AKAP1 (AKAP1 Human)

Use

Lentiviral

Tags

Myc

Mutation

gRNA-resistant silent mutations

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1 AMPK αKD(27-290aa)

Plasmid

#227853

Purpose

To express in E. coli AMPK αKD(27-290aa)

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

AMPK (PRKAA1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 27-290 only

Available Since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-EIF2AK2-ts2

Plasmid

#174248

Purpose

EIF2AK2 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

EIF2AK2 shRNA (EIF2AK2 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-EIF2AK2-ts3

Plasmid

#174249

Purpose

EIF2AK2 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

EIF2AK2 shRNA (EIF2AK2 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-MAP2K4

Plasmid

#221295

Purpose

Expression of MAP2K4 (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

MAP2K4 (pLxIS) (Map2k4 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Parkin(C431N)-A92mKO2

Plasmid

#213545

Purpose

Transient mammalian expresssion of ligase-dead C431N Parkin, internally tagged with mKO2

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Expression

Mammalian

Mutation

C431N mutation in Parkin

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLv-EF1a-Parkin(R104A)-A92mKO2

Plasmid

#213556

Purpose

Lentiviral vector for stable expression of R104A Parkin, internally tagged with mKO2

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Lentiviral

Expression

Mammalian

Mutation

R104A mutation in Parkin

Promoter

EF1a

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLv-CMV(trunc)-Parkin-A92mKO2

Plasmid

#213549

Purpose

Lentiviral vector for stable low expression of Parkin, internally tagged with mKO2

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Truncated CMV

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLv-EF1a-Parkin(N273K)-A92mKO2

Plasmid

#213555

Purpose

Lentiviral vector for stable expression of N273K Parkin, internally tagged with mKO2

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Lentiviral

Expression

Mammalian

Mutation

N273K mutation in Parkin

Promoter

EF1a

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLv-EF1a-Parkin(W403A)-A92mKO2

Plasmid

#213558

Purpose

Lentiviral vector for stable expression of W403A Parkin, internally tagged with mKO2

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Lentiviral

Expression

Mammalian

Mutation

W403A mutation in Parkin

Promoter

EF1a

Available Since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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ZMYND8-2A-neo

Plasmid

#190619

Purpose

Lentiviral expression plasmid of human ZMYND8, neomycin selection

Depositor

Junwei Shi

Article

Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1

Insert

ZMYND8 (ZMYND8 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

synonymous mutation for sgRNA resistance at aa214…

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-chPKCdelta/eDHFR(69K6)-EGFP

Plasmid

#178857

Purpose

Mammalian expression of PKCδ-eDHFR(69K6) chimera fused to EGFP

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

PKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-EGFP (PRKCD Human)

Expression

Mammalian

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-chPKCdelta/eDHFR(69K6)-miRFP670

Plasmid

#178858

Purpose

Mammalian expression of PKCδ-eDHFR(69K6) chimera fused to miRFP670

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

PKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-miRFP670 (PRKCD Human)

Expression

Mammalian

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_MAPK9-P2A-Hygro_Barcode

Plasmid

#170238

Purpose

Barcoded lentiviral vector to express MAPK9 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

MAPK9 (MAPK9 Human)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-OLIG2wt-IDR

Plasmid

#145250

Purpose

Expresses fusion of GAL4 DNA-binding domain and OLIG2wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

OLIG2wt-IDR (OLIG2 Human)

Expression

Mammalian

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3_nmPKA-CαN-mPAGFP

Plasmid

#114130

Purpose

Mammalian expression of the G2A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP, with G2A mutation (Prkaca Mouse)

Expression

Mammalian

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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