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Plasmid#157740Purposeempty vector; N-terminal PelB signal sequence; potential C-terminal His6; T7 promoter; like pET-20b, but additionally avoids added residues in final protein since cloning uses BseRI restriction sitesDepositorTypeEmpty backboneUseTagsPelB signal sequence and possible His6-tag (adds …ExpressionBacterialMutationPromoterT7Available sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only
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pREPK219H
Plasmid#51492PurposepREP with a mutation in the ATPase domain (K219H). This is predicted to result in loss of geminivirus replication.DepositorInsertRep/RepA
UsePlant expression t-dnaTagsExpressionMutationchanged lysine 219 to histidinePromoter2x35SAvailable sinceMay 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 - mSRSF6
Plasmid#120420Purposeoverexpression of mouse Srsf6DepositorInsertSRSF6 (Srsf6 Mouse)
UseTagsExpressionMammalianMutationPromoterCMV immediate/earlyAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
GEARBOCS-SPARCL1 KO
Plasmid#218182PurposeTo KO Hevin in astrocytesDepositorInsertsgRNA (Sparcl1 Mouse)
UseAAV and CRISPRTagsExpressionMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pES133
Plasmid#127552PurposeVariant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13DepositorInsertF5-flanked tetracycline resistance cassette
UseTagsExpressionBacterialMutationMultiple SNPs in tetA (originally from pBR322) to…PromoterAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
HT-mGold
Plasmid#183986PurposeExpresses fluorescent HaloTag7 fusion protein in mammalian cells in the whole cell.DepositorInsertHaloTag-mGold
UseTagsHaloTag, mGoldExpressionMammalianMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sup35 ScNM A230C His7
Plasmid#15598DepositorInsertSup35 NM (SUP35 Budding Yeast)
UseTags7xHisExpressionBacterialMutationA230C. Alanine 230 mutated to cysteine.PromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRVV661
Plasmid#108282PurposeDonor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9DepositorInsertPhiC31 attP sites
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2+methylated-reporter
Plasmid#164491PurposeReporter to test methylated mRNA stability, for mRNA injectionDepositorInsertA-less CDS, HA tag, 12x m6A motif 3'UTR
UseProduction of mrna for zebrafish injection.TagsHA tagExpressionMutationPromoterSP6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTF101.3
Plasmid#134772PurposeA binary plasmid containing double CaMV 35S promoter to drive bialophos resistance gene (bar) for plant transformation.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLSLGUS
Plasmid#51517PurposepLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmidDepositorInsertsGUS
3kb filler sequence
UsePlant t-dna plasmidTagsNLS and flagExpressionMutationPromotervirion-sense LIR and 2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRVV584
Plasmid#108280PurposeDonor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9DepositorInsertPhiC31 attP sites
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRVV669
Plasmid#108283PurposeDonor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9DepositorInsertPhiC31 attP sites
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRVV613
Plasmid#108281PurposeDonor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9DepositorInsertPhiC31 attP sites
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLSLT
Plasmid#51498PurposepLSL with T30 TALEN followed by us:NPTII repair template, Rep is not included on this plasmidDepositorInsertsT30 TALEN pair
us:NPTII repair template
UsePlant t-dna plasmidTagsExpressionMutationPromotervirion-sense LIR and 2x35SAvailable sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
Sup35 Sc/Ca NM Chimera WT His7 Cys
Plasmid#15600DepositorInsertSup35 NM (SUP35 Budding Yeast, Candida albicans)
UseTags7xHis and CysteineExpressionBacterialMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
p35SZ
Plasmid#51513PurposepMDC32 with Zif268:FokI followed by approximately 3kb of filler sequenceDepositorInsertsZif268:FokI
3kb filler sequence
UsePlant expression t-dnaTagsNLSExpressionMutationPromoter2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
HOXA9-micro-MSCV
Plasmid#20976DepositorInsertHOXA9 (Hoxa9 Mouse)
UseRetroviralTagsExpressionMammalianMutationMutations to remove binding sites for miR-145, le…PromoterAvailable sinceAug. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
fabp10a:FLAG-SpiCee-mRFP1; cryaa:mCherry
Plasmid#230062PurposeExpression of genetically encoded calcium chelator in zebrafish hepatocytes.DepositorInsertSpiCee
UseZebrafish expressionTagsFLAG and mRFP1ExpressionMutationPromoterfabp10aAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBSII-FRT-MCS
Plasmid#205988PurposeVector containing FRT and FRT-F3 sitesDepositorTypeEmpty backboneUsePcr vectorTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
LEGO-pDHS-AP1x6-DUSP5
Plasmid#217418PurposeLuciferase expression vector with 6 AP-1 sites and the full length DUSP5 promoter, and a chromatin priming element. Alias: LEGO-DUSP5p-AP-1x6DepositorInsertLuciferase, GFP
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterHuman DUSP5 promoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTF101.4
Plasmid#134773PurposeA binary plasmid containing nopaline synthase promoter to drive bialophos resistance gene (bar) for plant transformation.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLSLGFP.R
Plasmid#51501PurposeSingle component LSL vector with GFP. Rep is expressed from the complementary sense LIR promoter. GFP is between LIR and SIR and is driven from a 2X35S promoter. Rep is within the repliconDepositorInsertGFP
UsePlant t-dna plasmidTagsExpressionMutationPromoter2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pmTurquoise2-Drp1
Plasmid#110946PurposeExpresses dynamin-like protein 1 fused to mTurquoise2DepositorInsertDynamin-1-like protein (DNM1L Human)
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
bRA89
Plasmid#100950PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherryC1 Human WIP
Plasmid#29573DepositorInsertWASP-interacting protein (WIPF1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFA104.PI.eGFP.WPRE.bGH
Plasmid#100896PurposeAAV expression of EGFP in astrocytesDepositorHas ServiceAAV5InsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterGFA104 (GFAP promoter)Available sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
bRA77
Plasmid#100953PurposeGAL1 driven Cas9. gRNA can be cloned into BplI sites.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLGB28
Plasmid#126617PurposepNBU2-derived shuttle vector for integration at attBT2 sites in Bacteroides chromosomes, inulin selection for use in anbitiotic resistant strainsDepositorTypeEmpty backboneUseUnspecified; Chromosomal integration suicide vect…TagsExpressionMutationPromoterAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only