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Showing: 2801 - 2840 of 8319 results

  1. pKT-PDGFRa-IRES-Katushka

    Plasmid
    #133081
    Purpose
    Expresses PDGFRa receptor and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Insert
    PDGFRa (Pdgfra Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    133081-plasmid-map-sequence-id-261534

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  2. AAVS1_Puro_H2BK_mNG_hSLBP(18-108)

    Plasmid
    #191529
    Purpose
    Donor for targeted integration of mNG_hSLBP(18-108) probe to the AAVS1 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    H2BK_mNG_hSLBP(18-108)
    Use
    CRISPR and Synthetic Biology
    Tags
    mNeonGreen (mNG)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191529-plasmid-map-sequence-id-378106

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  4. MIG-REST

    Plasmid
    #91898
    Purpose
    Expression of mouse REST
    Depositor
    Julien Sage
    Insert
    REST (Rest Mouse)
    Use
    Retroviral
    Tags
    ires-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    MSCV
    Available since
    June 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    91898-plasmid-map-sequence-id-180660

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  5. Sperm whale Mb 64Y68F in pVP80K

    Plasmid
    #86956
    Purpose
    Increases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).
    Depositor
    John Olson
    Insert
    Mb 64Y68F
    Use
    Tags
    Expression
    Bacterial
    Mutation
    64Y68F
    Promoter
    T5
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    86956-plasmid-map-sequence-id-176792

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  6. pLIX403-EBFP2-ccdB-Blast

    Plasmid
    #158557
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158557-plasmid-map-sequence-id-309870

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  7. pB-CAG-PE2-NatMX

    Plasmid
    #158567
    Purpose
    Piggybac vector that allows for the generation of PE2-expressing stable cell lines
    Depositor
    Alejandro Chavez
    Insert
    PE2
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158567-plasmid-map-sequence-id-309882

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  8. pJK633

    Plasmid
    #72397
    Purpose
    Produces Escherichia coli aminoimidazole ribonucleotide synthetase, N-terminal His6 tag (H6EcPurM)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole synthetase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    72397-plasmid-map-sequence-id-134629

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  9. mem-mCherry-pTRIP

    Plasmid
    #163521
    Purpose
    Used to construct lentivirus to express membrane-targeted mCherry in mammalian cells
    Depositor
    Thomas Weber
    Insert
    membrane targeted mCherry
    Use
    Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163521-plasmid-map-sequence-id-321568

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  10. pLXIN2-RFP

    Plasmid
    #99205
    Purpose
    RFP expressing bicistronic retroviral vector
    Depositor
    Saroj Mathupala
    Insert
    Red fluorescent protein
    Use
    Retroviral; Bicistronic retroviral expression vec…
    Tags
    Expression
    Mammalian
    Mutation
    The dsRed cDNA was PCR amplified off AddGene plas…
    Promoter
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    99205-plasmid-map-sequence-id-192441

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  11. pAG416GAL-EMCV_3C

    Plasmid
    #201941
    Purpose
    Expresses EMCV 3C protease from a GAL promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    EMCV 3C protease (EMCVgp1 )
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    Aug. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    201941-plasmid-map-sequence-id-403575

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  12. pDTA-5

    Plasmid
    #128845
    Purpose
    Gateway-compatible p15A origin plasmid with a DTA negative selection cassette for targeting vector construction, I-CeuI, low copy number
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    128845-plasmid-map-sequence-id-252002

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  13. pHH0103 NEDD4 WW domain #3

    Plasmid
    #104210
    Purpose
    Bacterial expression of WW domain #3 from NEDD4
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    NEDD4 WW domain #3 (NEDD4 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    104210-plasmid-map-sequence-id-204199

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  14. pGAL-flp(H305L)-NAT

    Plasmid
    #160380
    Purpose
    overexpression of Flp recombinase step-arrest mutant in yeast
    Depositor
    John McCusker
    Insert
    FLP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    H305L
    Promoter
    GAL
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    160380-plasmid-map-sequence-id-314503

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  15. pHH0103 NEDD4L WW domain #2

    Plasmid
    #104205
    Purpose
    Bacterial expression of WW domain #2 from NEDD4L
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    NEDD4L WW domain #2 (NEDD4L Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104205-plasmid-map-sequence-id-203673

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  16. pJK615

    Plasmid
    #71699
    Purpose
    Produces E. coli thioredoxin with a C-terminal His6 tag (EcTrxAH6)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71699-plasmid-map-sequence-id-137034

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  17. pUA97

    Plasmid
    #69340
    Purpose
    It encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategy
    Depositor
    Oliver Hobert
    Insert
    ceBFP SEC 3xFlag
    Use
    Tags
    3xFlag and ceBFP (mTagBFP)
    Expression
    Worm
    Mutation
    sqt-1(e1350)
    Promoter
    Available since
    Dec. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    69340-plasmid-map-sequence-id-133241

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  18. Preprotachykinin A

    Plasmid
    #130664
    Purpose
    Expresses human Preprotachykinin A in mammalian cells
    Depositor
    Michael Kressel
    Insert
    Preprotachykinin A (TAC1 Human)
    Use
    Tags
    Fusion Red
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    130664-plasmid-map-sequence-id-254047

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  19. pGHE-PdNIP2-1

    Plasmid
    #108447
    Purpose
    PdNIP2-1 gene from Date Palm (Phoenix dactylifera) cloned in pGHE. Can be used for Xenopus oocyte assay. The vector has Xenopus globin 3UTR and 5UTR, pT7, and polyA.
    Depositor
    Richard Bélanger
    Insert
    PdNIP2-1
    Use
    Tags
    Expression
    Mutation
    Promoter
    pT7
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    108447-plasmid-map-sequence-id-274297

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  20. pHH0103 NEDD4 WW domain #1

    Plasmid
    #104208
    Purpose
    Bacterial expression of WW domain #1 from NEDD4
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    NEDD4 WW domain #1 (NEDD4 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    104208-plasmid-map-sequence-id-204367

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  21. pKSB- 3xP3-GFPsv40 GGGG AAGA

    Plasmid
    #174531
    Purpose
    3xP3-GFP fluorescence marker cassette for gene knock-in in mosquitoes
    Depositor
    Eric Marois
    Insert
    3xP3-GFP BsaI cassette
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    3xP3
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    174531-plasmid-map-sequence-id-343226

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  22. pLinker-BirA-3xHA-HXGPRT-loxP

    Plasmid
    #86668
    Purpose
    for generation of BirA tagged strains using CRISPR tagging technology
    Depositor
    David Sibley
    Insert
    Linker-BirA-3xHA-HXGPRT 3'UTR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    86668-plasmid-map-sequence-id-190141

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  23. pE-SUMO CRYGD

    Plasmid
    #80755
    Purpose
    Expression of human gamma D-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYGD (CRYGD Human)
    Use
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80755-plasmid-map-sequence-id-153570

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  24. pHH0103 NEDD4 WW domain #4

    Plasmid
    #104211
    Purpose
    Bacterial expression of WW domain #4 from NEDD4
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    NEDD4 WW domain #4 (NEDD4 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    104211-plasmid-map-sequence-id-201499

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  25. pExp-Trx

    Plasmid
    #112577
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Trx (thioredoxin (trxA) from E. coli fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-TrxA and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112577-plasmid-map-sequence-id-217212

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  26. pLinker-6xHA-HXGPRT-LoxP

    Plasmid
    #86552
    Purpose
    The plasmid is used for endogenous tagging using CRISPR technology in Toxoplasma
    Depositor
    David Sibley
    Insert
    Linker-6xHA-HGPRT-LoxP
    Use
    Expression in toxoplasma gondii
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    86552-plasmid-map-sequence-id-190138

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  27. pAG426GAL-KUNV_NS2B-GS-NS3

    Plasmid
    #203506
    Purpose
    Expresses KUNV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    KUNV NS2B-GS-NS3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    July 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    203506-plasmid-map-sequence-id-402629

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  28. HT7-mTurquoise2-KDEL

    Plasmid
    #188571
    Purpose
    Expresses fluorescent HaloTag7-mTurquoise2 fusion protein in mammalian cells with localization in the endoplasmic reticulum.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    HaloTag7
    Use
    Tags
    KDEL and mTurquoise2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    188571-plasmid-map-sequence-id-369501

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  29. pB6-ROSA26-DTA-Capecchi-for

    Plasmid
    #125743
    Purpose
    B6J ROSA26 targeting vector homology arms, Capecchi configuration, DTA, I-CeuI, forward cloning sites, low copy number
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125743-plasmid-map-sequence-id-247902

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  30. PB CAG-sfGFP-iCre-ERT2

    Plasmid
    #137856
    Purpose
    CAG-sfGFP-iCre-ERT2 piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    sfGFP-iCre-ERT2
    Use
    Cre/Lox; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    137856-plasmid-map-sequence-id-316180

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  31. Flag-TRIM24-F869D

    Plasmid
    #28141
    Depositor
    Michelle Barton
    Insert
    TRIM24 (TRIM24 Human)
    Use
    Tags
    FLAGX2
    Expression
    Mammalian
    Mutation
    A489V, F869D; aa 308 and 309 mutated for shRNA re…
    Promoter
    Available since
    Feb. 10, 2012
    Availability
    Academic Institutions and Nonprofits only

  32. pET32a-Trx-His6-TEV_SacB

    Plasmid
    #164588
    Purpose
    Bacterial expression plasmid with sacB gene. Suitable for LIC cloning of a gene insert replacing the sacB.
    Depositor
    Ernst Schonbrunn
    Insert
    TEV-SacB
    Use
    Tags
    Trx-His6 followed by TEV site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    164588-plasmid-map-sequence-id-337962

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  33. pET21a(+)_C.jejuni m23 peptidase

    Plasmid
    #83297
    Purpose
    Expresses m23 peptidase from Campylobacter jejuni
    Depositor
    SeWon Suh
    Insert
    ERS445056_01343
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    deleted amino acid 1-20
    Promoter
    Available since
    Nov. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    83297-plasmid-map-sequence-id-164865

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  34. pCMV-PE2-pegRNA-tetra-com vector

    Plasmid
    #136271
    Purpose
    Expresses PE2 and Tetra-com modified pegRNA cassette
    Depositor
    Baisong Lu
    Insert
    PE2 and pegRNA cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    136271-plasmid-map-sequence-id-273274

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  35. CbSBS-Flag/TRRAP(S722F)

    Plasmid
    #32105
    Depositor
    Yardena Samuels
    Insert
    Transformation/transcription domain-associated protein (S722F) (TRRAP Human)
    Use
    Tags
    Flag 1x
    Expression
    Mammalian
    Mutation
    Changes serine 722 to Phe
    Promoter
    CMV
    Available since
    Nov. 14, 2011
    Availability
    Academic Institutions and Nonprofits only

  36. pcDNA3.1 myc-PITPalpha

    Plasmid
    #58277
    Purpose
    mammalian expression of human PITP alpha
    Depositor
    Shamshad Cockcroft
    Insert
    PITP alpha (PITPNA Human)
    Use
    Tags
    myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  37. pAct-Fok1:dCas9

    Plasmid
    #62211
    Purpose
    Expresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous high specificity genome engineering in Drosophila melanogaster.
    Depositor
    Simon Bullock
    Insert
    Fok1-dCas9
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    act5C
    Available since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    62211-plasmid-map-sequence-id-101870

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  38. p2Bac.Myc.p44

    Plasmid
    #39220
    Depositor
    Aziz Sancar
    Insert
    p44 (GTF2H2 )
    Use
    Tags
    Myc
    Expression
    Insect
    Mutation
    Promoter
    Pp10
    Available since
    Aug. 24, 2012
    Availability
    Academic Institutions and Nonprofits only

  39. pNB3033

    Plasmid
    #113397
    Purpose
    Ty3 Gag3-His tagged, codon optimized for expression in E. coli; . IPTG inducible
    Depositor
    Suzanne Sandmeyer
    Insert
    Ty3 Gag3 codon optimized for E. coli expression
    Use
    Tags
    Gag3-6XHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    113397-plasmid-map-sequence-id-217374

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  40. pmr101A-cPr-mCherry (ErmR)

    Plasmid
    #177206
    Purpose
    Plasmid with p15A origin for constitutive mCherry expression in E. coli
    Depositor
    Alexey Merz
    Insert
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic constitutive promoter
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177206-plasmid-map-sequence-id-348606

    Enlarge

Showing: 2801 - 2840 of 8319 results