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pCMV-T7-AcrIIA16_Lmo-NLS(SV40) (KAC508)

Plasmid

#134332

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Lmo with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA16_Lmo

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA17_Sga-NLS(SV40) (KAC92)

Plasmid

#134334

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Sga with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA17_Sga

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA18_Sma-NLS(SV40) (KAC95)

Plasmid

#134335

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA18_Sma with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA18_Sma

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA19_Ssim-NLS(SV40) (KAC97)

Plasmid

#134336

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Ssim with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA19_Ssim

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-AcrIIA18_Sga-NLS(SV40) (KAC214)

Plasmid

#134338

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA18_Sga with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA18_Sga

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-AcrIIA19_Spse-NLS(SV40) (KAC215)

Plasmid

#134339

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Spse with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA19_Spse

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT45_GalL_nCDA1-BE3

Plasmid

#145038

Purpose

Expresses nCDA1-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1-BE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pRAN357

Plasmid

#120898

Purpose

E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target protein

Depositor

David S. Weiss, Craig Ellermeier

Article

Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.

Insert

cyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pPN460

Plasmid

#137878

Purpose

PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRa;mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

POGZ (POGZ Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pPK1E-1-gfp

Plasmid

#48133

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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QuasAr-P2A-GCaMP

Plasmid

#154082

Purpose

Independent expression of QuasAr and GCaMP under human synapsin 1 promoter

Depositor

Michael Hoppa

Article

Cho et al Proc Natl Acad Sci U S A. 2020 Nov 24;11

Insert

QuasAr-P2A-GCaMP6f

Use

AAV

Tags

Expression

Mammalian

Mutation

Changed Tyrosine 327 to Alanine

Promoter

human synapsin 1

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST53-Arl13b20

Plasmid

#40876

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted Arl N-terminal domain; added stop codon

Promoter

CMV

Available since

Nov. 29, 2012

Availability

Academic Institutions and Nonprofits only
LP454

Plasmid

#90185

Purpose

pFLAG-CMV2 with cDNA encoding HsNPHP4 aa residues 1-649

Depositor

Lotte Pedersen

Article

Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

Insert

NPHP4 (NPHP4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

aa residues 1-649

Promoter

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pJT79_GalL_nCDA1Δ192-BE3

Plasmid

#145045

Purpose

Expresses nCDA1Δ192-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1Δ192-BE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

pmCDA1(1-192AA); spCas9(D10A)

Promoter

GalL

Available since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pECE M2-SNX17 S437A

Plasmid

#69812

Purpose

Expresses M2-SNX17 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Sorting nexin-17 (SNX17 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pPK1E-1+t-gfp

Plasmid

#48131

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF5/FRT-DEST EVC2dW-TEV-YFP-Flag

Plasmid

#41006

Depositor

Rajat Rohatgi

Article

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

Ellis van Creveld syndrome 2 (EVC2 Mouse)

Use

Tags

TEV-YFP-Flag

Expression

Mammalian

Mutation

deleted amino acids 1177-1220

Promoter

Available since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only
pPK1E-2+t-gfp

Plasmid

#48132

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Puro-CMV>{5-HT6-R_GECO1.1}

Plasmid

#232879

Purpose

Expresses the 5-HT6R coupled to the RGECO1 calcium sensor

Depositor

Paul DeCaen

Article

DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

Insert

5-Hydroxytryptamine Receptor 6 (HTR6 Human)

Use

Lentiviral

Tags

RGECO1.1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Puro-CMV>{ARL13B_6gly_GFP_6Gly_R_GECO1}

Plasmid

#232880

Purpose

Expresses the ARL13B coupled to GFP and RGECO1 calcium sensor.

Depositor

Paul DeCaen

Article

DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

Insert

ADP-ribosylation factor-like protein 13B (ARL13B Human)

Use

Lentiviral

Tags

GFP and RGECO1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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