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We narrowed to 10,333 results for: SEC

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Showing: 1401 - 1420 of 10333 results

  1. pcDNA3.1+CMV-Ins-GLuc

    Plasmid
    #89929
    Purpose
    Expresses Insulin-GLuc from the CMV promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.
    Depositor
    Melanie Cobb
    Article
    Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E
    Insert
    human insulin-Gaussia-Luciferase (INS Human, Synthetic)
    Expression
    Mammalian
    Mutation
    DNA sequence for Gaussia luciferase was humanized…
    Promoter
    CMV
    Available Since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    89929-plasmid-map-sequence-id-255704

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  2. pLIB_3xFlag-His-nsp5CS-nsp9 (SARS-CoV-2)

    Plasmid
    #169177
    Purpose
    Baculoviral transfer vector to express SARS-CoV-2 nsp9 with nsp5-cleavable 3xFlag-His6 tag in insect cells
    Depositor
    John Diffley
    Article
    Milligan et al Biochem J. 2021 Jul 16;478(13):2499-2515
    Insert
    3xFlag-His-nsp5CS-nsp9 (ORF1ab SARS-CoV-2)
    Tags
    3xFlag-His6 (nsp5-cleavable)
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169177-plasmid-map-sequence-id-333223

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  3. pLIB_3xFlag-His-PLpro (nsp3) (SARS-CoV-2)

    Plasmid
    #169194
    Purpose
    Baculoviral transfer vector to express SARS-CoV-2 papain-like protease domain (nsp3) in insect cells
    Depositor
    John Diffley
    Article
    Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531
    Insert
    3xFlag-His6-PLpro (ORF1ab Synthetic, SARS-CoV-2)
    Tags
    3xFlag-His6
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169194-plasmid-map-sequence-id-333980

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  4. pcDNA3.1-GSDMD_1-270

    Plasmid
    #218229
    Purpose
    Gasdermin1-270 is a pore-forming GSDMD formed by SARS-CoV-2 3CLpro cleavage. Transfection in HEK293 cells showed GSDMD 1-270 pores secrete 3CLpro, nucleocapsid, and in excess, kill cells by pyroptosis
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    GSDMD (GSDMD Human)
    Tags
    flag
    Expression
    Mammalian
    Mutation
    construct only codes for amino acids 1-270
    Available Since
    July 1, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    218229-plasmid-map-sequence-id-430564

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  5. pcDNA3.1-GSDMD_1-275

    Plasmid
    #218230
    Purpose
    Gasdermin-D_1-275 is a pore-forming GSDMD formed by cleavage by host caspases. Transfection in HEK293 cells showed GSDMD 1-275 pores secrete 3CLpro, nucleocapsid; in excess, kills cells by pyroptosis
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    GSDMD (GSDMD Human)
    Tags
    flag
    Expression
    Mammalian
    Mutation
    construct only codes for amino acids 1-275
    Available Since
    July 1, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    218230-plasmid-map-sequence-id-430565

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  6. pF1-Hs Cdk1/cyclin B1/Cks1

    Plasmid
    #177329
    Purpose
    Co-expression of the human active Cdk1/cyclin B1/Cks1 kinase complex
    Depositor
    Andreas Boland
    Article
    Yu et al Nature. 2021 Aug;596(7870):138-142. doi:
    Inserts
    Cyclin-dependent kinase 1 (CDK1 Human)
    G2/mitotic-specific cyclin-B1 (CCNB1 Human)
    Cyclin-dependent kinases regulatory subunit 1 (CKS1B Human)
    Tags
    2 x Strep-tag and 8 x His
    Expression
    Insect
    Promoter
    p10 and polh
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    177329-plasmid-map-sequence-id-348397

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  7. pJH127

    Plasmid
    #162483
    Purpose
    SEC plasmid containing LG1 homology arms and myo-2p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.
    Depositor
    Bob Goldstein
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    myo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Use
    CRISPR
    Tags
    myo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Expression
    Worm
    Available Since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    162483-plasmid-map-sequence-id-320838

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  8. pJH130

    Plasmid
    #162484
    Purpose
    SEC plasmid containing LG1 homology arms and myo-3p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.
    Depositor
    Bob Goldstein
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    myo-3p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Use
    CRISPR
    Tags
    myo-3p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Expression
    Worm
    Available Since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    162484-plasmid-map-sequence-id-320834

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  9. pJH126

    Plasmid
    #162482
    Purpose
    SEC plasmid containing LG1 homology arms and vha-8p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.
    Depositor
    Bob Goldstein
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    vha-8p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Use
    CRISPR
    Tags
    vha-8p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Expression
    Worm
    Available Since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    162482-plasmid-map-sequence-id-320829

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  10. pAc5.1B-HA-SBP_J

    Plasmid
    #146621
    Purpose
    Insect Expression vector
    Depositor
    Elisa Izaurralde
    Type
    Empty backbone
    Expression
    Insect
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146621-plasmid-map-sequence-id-421635

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  11. pAc5.1B-V5-SBP_J

    Plasmid
    #146633
    Purpose
    Insect Expression vector
    Depositor
    Elisa Izaurralde
    Type
    Empty backbone
    Expression
    Insect
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146633-plasmid-map-sequence-id-421638

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  12. pAc5.1B-MS2_V

    Plasmid
    #147737
    Purpose
    Insect Expression vector
    Depositor
    Elisa Izaurralde
    Type
    Empty backbone
    Expression
    Insect
    Available Since
    March 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    147737-plasmid-map-sequence-id-357450

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  13. pMT-CTAP_H

    Plasmid
    #146453
    Purpose
    Insect Expression vector
    Depositor
    Elisa Izaurralde
    Type
    Empty backbone
    Expression
    Insect
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146453-plasmid-map-sequence-id-288898

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  14. pMT-NTAP_H

    Plasmid
    #146454
    Purpose
    Insect Expression vector
    Depositor
    Elisa Izaurralde
    Type
    Empty backbone
    Expression
    Insect
    Available Since
    March 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    146454-plasmid-map-sequence-id-355944

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  15. pAc5.1B-EGFP-MBP_V

    Plasmid
    #147736
    Purpose
    Insect Expression vector
    Depositor
    Elisa Izaurralde
    Type
    Empty backbone
    Expression
    Insect
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147736-plasmid-map-sequence-id-290183

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  16. pAc5.1B-HA-SBP-MBP_J

    Plasmid
    #146623
    Purpose
    Insect Expression vector
    Depositor
    Elisa Izaurralde
    Type
    Empty backbone
    Expression
    Insect
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146623-plasmid-map-sequence-id-289069

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  17. pAc5.1B-lambdaN_A

    Plasmid
    #145850
    Purpose
    Insect Expression of lambdaN
    Depositor
    Elisa Izaurralde
    Insert
    lambdaN
    Expression
    Insect
    Available Since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    145850-plasmid-map-sequence-id-288295

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  19. pAc5.1C-Fluc-EGFP_P

    Plasmid
    #147174
    Purpose
    Insect Expression of EGFP
    Depositor
    Elisa Izaurralde
    Insert
    EGFP
    Expression
    Insect
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147174-plasmid-map-sequence-id-289621

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Showing: 1401 - 1420 of 10333 results