We narrowed to 7,515 results for: Ski;

1. PB-tight-DDdCas9VP192-T2A-GFP-IRES-Neo

   Plasmid

   #102889

   Purpose

   PiggyBac transposon system construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE-tight

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA1 Flag ATXN1[2Q]

   Plasmid

   #33235

   Depositor

   Huda Zoghbi

   Article

   Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   insert contains 2Q (2 glutamines) and there isno …

   Promoter

   CMV

   Available Since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWPI-GFP-3xFLAG

   Plasmid

   #201639

   Purpose

   Over-expression of 3xFLAG-tagged GFP

   Depositor

   Vamsi Mootha, Alexis Jourdain

   Article

   Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. CAS9PBKS

   Plasmid

   #68371

   Purpose

   CbAkt promoter driven Cas9-2A-GFP (from px458)

   Depositor

   Eric-Paul Bennett

   Article

   Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

   Insert

   Cas9
   

   Use

   Tags

   2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CbAkt

   Available Since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRARE-MBP-DEST

   Plasmid

   #84650

   Purpose

   Gateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.

   Depositor

   Neil Olszewski

   Article

   Steiner et al Plant Cell. 2012 Jan;24(1):96-108. doi:

   Type

   Empty backbone

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. p205-5'-Switch-ON

   Plasmid

   #217884

   Purpose

   Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

   Depositor

   Ulrich Elling

   Article

   Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB9v1

   Plasmid

   #210028

   Purpose

   Contains Cas9 gRNA cloning site, inducible Cas9, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas9
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   SV40 NLS and nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. U6GRNA

   Plasmid

   #68370

   Purpose

   gRNA scaffold with hU6 promoter

   Depositor

   Eric-Paul Bennett

   Article

   Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

   Plasmid

   #69534

   Purpose

   DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

   Insert

   DD-dCas9VP192-T2A-EGFP
   

   Use

   Tags

   C-term T2A-EGFP and DHFR Destabilised Domain

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Nov. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJEP300-pAAV-CMV-MCS2-pA

    Plasmid

    #112138

    Purpose

    CMV driven Multi Cloning Site-2

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    N/A
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGG-NFIX2

    Plasmid

    #112699

    Purpose

    Expression of NFIX2 protein and GFP

    Depositor

    Richard Gronostajski, Francois Guillemot

    Article

    Martynoga et al Genes Dev. 2013 Aug 15;27(16):1769-86. d

    Insert

    Nuclear Factor I X2 (Nfix Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-Cluc-RMA

    Plasmid

    #189624

    Purpose

    Expresses Cluc-RMA under the hSyn promoter. Cluc-RMA for monitoring neuronal transduction.

    Depositor

    Jerzy Szablowski

    Article

    Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    Cypridina luciferase fused to Fc
    

    Use

    AAV and Luciferase

    Tags

    Cluc and IgG1 Fc

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. p207-Switch-ON (FRT)

    Plasmid

    #217886

    Purpose

    Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1a-DIO-H2B.APEX-P2A-EGFP

    Plasmid

    #182825

    Purpose

    Cre-dependent expression of H2B-APEX fusion

    Depositor

    Yevgenia Kozorovitskiy

    Article

    Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

    Insert

    H2B.APEX-P2A-EGFP
    

    Use

    AAV

    Tags

    EGFP reporter (P2A)

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-CAG-DDdCas9VP192-T2A-GFP-IRES-Neo

    Plasmid

    #102885

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQCXIP-GFP-LmnB1

    Plasmid

    #164249

    Purpose

    retroviral plasmid for GFP-LmnB1

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    LMNB1 (LMNB1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPB12A

    Plasmid

    #210035

    Purpose

    Contains cloning site for Cas12a gRNA cassette, inducible Cas12a, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas12a
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLEX-FHH-MTORKinaseDomain

    Plasmid

    #120708

    Purpose

    Expresses Flag-HA-6xHIS-Mtor Kinase Domain fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    MTOR Kinase Domain (MTOR Human)
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Amino acids 1967-2549

    Promoter

    CMV

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLENTI-CMV-GFP-TREX1 delta C (aa1-235) BLAST

    Plasmid

    #164229

    Purpose

    lentiviral plasmid for GFP-hTREX1 delta C (aa1-235)

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 235-314

    Promoter

    CMV

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLEX Myc-HA

    Plasmid

    #229499

    Purpose

    For lentiviral expression of Myc coding sequence with an HA tag.

    Depositor

    Davide Ruggero

    Article

    Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

    Insert

    MYC
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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