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pCMV-HA-JMJD3

Plasmid

#24167

Purpose

Mammalian expression of human JMJD3 with HA tag

Depositor

Kristian Helin

Article

Agger et al Nature. 2007 Oct 11. 449(7163):731-4.

Insert

JMJD3 (KDM6B Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

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HA-Halotag-FRB-EX-nes_pLX304

Plasmid

#120913

Purpose

Lentiviral vector expressing HA-Halotag-FRB-EX-NES in mammalian cytosol

Depositor

Alice Ting

Article

Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

Insert

HA-Halotag-FRB-EX-NES

Use

Lentiviral

Expression

Mammalian

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pmBaoJin-Tubulin

Plasmid

#229566

Purpose

Expresses mBaoJin-tubulin fusion in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mBaoJin

Expression

Mammalian

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-EF1a-Zim3-dCas9-loxP-P2A-EGFP-loxP

Plasmid

#188773

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

EF1a

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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mEmerald-Nesprin3-C-18

Plasmid

#54203

Purpose

Localization: Nuclear Membrane , Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Nesprin3 (SYNE3 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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cfSGFP2-col4a1

Plasmid

#160317

Purpose

cfSGFP2-tagged collagen4a1 for mammalian expression

Depositor

Ikuo Wada

Article

Matsui et al Cell Struct Funct. 2020 Jul 23;45(2):107

Insert

COL4A1 (COL4A1 Human)

Tags

cfSGFP2

Expression

Mammalian

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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peSpCas9(1.1)-2×sgRNA (IFT88, donor)

Plasmid

#80769

Purpose

Expresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Insert

IFT88 gRNA#1 (IFT88 Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUB1 (full-length, aa 1-271)

Plasmid

#61420

Purpose

Expresses human OTUB1 (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUB1 (Ubiquitin thioesterase OTUB1) (OTUB1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR2b-mScarlet

Plasmid

#207961

Purpose

Gateway entry vector with the mScarlet fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

Insert

mScarlet

Use

Gateway entry vector

Promoter

None

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC7A11

Plasmid

#132244

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC7A11 (SLC7A11 Human)

Expression

Mammalian

Available Since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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