We narrowed to 6,265 results for: pcDNA 3.1

Showing: 1421 - 1440 of 6265 results

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pcDNA3.1-hLbCpf1(TYCV) (pY230)

Plasmid

#89355

Purpose

Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

Depositor

Feng Zhang

Article

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

G532R/K595R

Promoter

CMV

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-AntiHer2-LC122-TAG

Plasmid

#226839

Purpose

Contains trastuzumab with a TAG codon at position 122 of the light chain for ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Osgood et al Bioconjug Chem. 2024 Apr 17;35(4):457-46

Inserts

anti-HER2 light chain
anti-HER2 heavy chain

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Mutation

amino acid 122 of light chain mutated to TAG

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-AntiHer2-HC197-TAG

Plasmid

#226838

Purpose

Contains trastuzumab with a TAG codon at position 197 of the heavy chain for ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Osgood et al Bioconjug Chem. 2024 Apr 17;35(4):457-46

Inserts

anti-HER2 light chain
anti-HER2 heavy chain

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Mutation

amino acid 197 of heavy chain mutated to TAG

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-JEDI-2P

Plasmid

#179458

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Insert

JEDI-2P

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-MQ1(WT)-EGFP

Plasmid

#89633

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR

Tags

3*FLAG and 6*His

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 ZoAl-ENT N-1F (R1103A)

Plasmid

#216548

Purpose

For expression of N-flag tagged ZoAl-ENT R2 protein in mammalian cells

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement protein

Tags

Flag

Expression

Mammalian

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-mutTornado-split-nLuc

Plasmid

#212707

Purpose

Tornado split nLuc with a mutated 3' ribozyme so that circularization cannot occur.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

mutTornado-CVB3-split-nLuc

Mutation

Deleted the partial 3'ribozyme sequence in T…

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/hChR2(H134R)-EYFP

Plasmid

#20940

Purpose

Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Expression

Mammalian

Mutation

H134R mutation in ChR2

Promoter

CMV

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag-HIF1B (#930)

Plasmid

#99916

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Chen et al Nature. 2016 Nov 3;539(7627):112-117. do

Insert

HIF1B (ARNT Human)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Myc-His(-)-HSulf-1

Plasmid

#13003

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

human sulfatase 1 (SULF1 Human)

Expression

Mammalian

Available Since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/opto-b2AR-EYFP

Plasmid

#20948

Purpose

Mammalian expression of opto-b2AR fused to EYFP for optical control of GPCR signaling

Depositor

Karl Deisseroth

Article

Airan et al Nature. 2009 Apr 23;458(7241):1025-9. do

Insert

opto-b2AR

Expression

Mammalian

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)

Plasmid

#117484

Purpose

Expresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cells

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

SpyCatcher002-6aa-sfCherry2(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG-SBP-STAG2 (WT)

Plasmid

#73963

Purpose

STAG2 cDNA (CCDS43990) wild type

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Tags

FLAG-SBP

Expression

Mammalian

Mutation

wild type

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-HLA-Flag-natT1R3

Plasmid

#113950

Purpose

mammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R3 (TAS1R3 Human)

Tags

FLAG and Signal/leader sequence from HLA class I …

Expression

Mammalian

Mutation

residues 21-852

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) mouse Egr3

Plasmid

#107998

Purpose

Expresses mouse Egr3 in mammalian cells

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr3 (Egr3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HaloAKAR2.1-T2A-EGFP

Plasmid

#216489

Purpose

Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and EGFP transfection marker in mammalian cells

Depositor

Jin Zhang

Article

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloAKAR2.1

Tags

T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HshnRNPA2-B1_Z

Plasmid

#148113

Purpose

Mammalian Expression of HshnRNPA2-B1

Depositor

Elisa Izaurralde

Insert

HshnRNPA2-B1 (HNRNPA2B1 Human)

Expression

Mammalian

Mutation

splicing variant of hnRNPA2 variant B1. Exon 6 an…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUG-DAP5-3XFLAG

Plasmid

#127331

Purpose

Express 3xFLAG tagged DAP5 protein from AUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

DAP5 (EIF4G2 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 (-) + SLNCR1 + 12x MS2 bs

Plasmid

#86828

Purpose

Expression of SLNCR1 lncRNA tagged with 12 copies of the MS2 stem-loop sequence

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Tat101(AD8)-Flag

Plasmid

#115777

Purpose

pCMV-Tat101AD8-Flag allows the expression of Tat101(AD8) protein with a C-terminal flag-tag.

Depositor

Damian Purcell

Article

Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

Insert

HIV-1 Tat protein (AD8)

Tags

Flag

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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