We narrowed to 29,857 results for: REP

1. pX330A-1x5

   Plasmid

   #58769

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-Bmp7

   Plasmid

   #163591

   Purpose

   Expresses BMP-7 in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmp7 (Bmp7 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pN1-QNA

   Plasmid

   #129309

   Purpose

   Negative control for ATP measurement

   Depositor

   Yasushi Okada

   Article

   Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

   Insert

   QUEEN-NA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. LMAPPW

   Plasmid

   #205657

   Purpose

   shRNAmir backbone for RNA interference, with Puromycin selection

   Depositor

   Dapeng Wang

   Article

   Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

   Insert

   miR-AB
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   mCMV

   Available Since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PylT(UAG) PylS mCherry-P2A-eGFP(150TAG)

   Plasmid

   #174526

   Purpose

   Dual-fluorescence reporter for quantifying PylT(UAG) decoding efficiency

   Depositor

   Yu-Hsuan Tsai

   Article

   Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

   Insert

   PylS
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. MAC_C_RYK

   Plasmid

   #187790

   Purpose

   MAC-tagged gene expression of human RYK

   Depositor

   Markku Varjosalo

   Article

   Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

   Insert

   RYK (RYK Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-MCS-BioID2-3xFLAG

   Plasmid

   #186812

   Purpose

   CAG promoter-driven expression of BioID2-fused protein (C terminus)

   Depositor

   Masahito Ikawa

   Article

   Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

   Type

   Empty backbone

   Use

   Tags

   BioID2-3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. myr-palm_2xmEYFP

   Plasmid

   #127811

   Purpose

   Homo-dimer mEYFP-mEYFP expression vector with plasma membrane localization signal

   Depositor

   Salvatore Chiantia

   Article

   Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

   Insert

   myristoylated-palmitoylated-mEYFP-mEYFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX330A-1x7

   Plasmid

   #58771

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDRF1-GW yomCherry-T2A-mTurquoise2

    Plasmid

    #118447

    Purpose

    Produces equimolar expression of yeast codon-optimized mCherry and mTq2

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    yomCherry-T2A-mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR - CaSR-EGFP

    Plasmid

    #191503

    Purpose

    A zebrafish CaSR-EGFP construct used for Gateway cloning.

    Depositor

    Michael Granato

    Article

    Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

    Insert

    CaSR-EGFP-SV40
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    none

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pN1-QUE2m

    Plasmid

    #129308

    Purpose

    QUEEN ATP sensor for 25degC

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

    Insert

    QUEEN-2m
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHSP70B-CP6-bsr

    Plasmid

    #165878

    Purpose

    Basic Blasticidin resistance cassette in Alpha1, lacks physical marker or attB site

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Basic Selection Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. S5_EcoRV_Ef1a_AscI

    Plasmid

    #128469

    Purpose

    Ef1a promoter

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    Ef1a promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME18ST-d5-HT2B

    Plasmid

    #53110

    Purpose

    Expression of Drosophila 5-HT2B

    Depositor

    Leslie Vosshall

    Article

    Gasque et al Sci Rep. 2013;3:srep02120. doi: 10.1038/

    Insert

    5HT2B (CG42796 Fly)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

16. EZH2_N_FS_v2_Donor

    Plasmid

    #79901

    Purpose

    N-terminal tagging of human EZH2 with 3xFLAG_Twin_Strep

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Type

    Empty backbone

    Use

    CRISPR and TALEN

    Tags

    3xFLAG_Twin_Strep

    Expression

    Mutation

    Promoter

    Available Since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAD/HisD-TagRFP675

    Plasmid

    #44274

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

    Insert

    TagRFP675
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

    Promoter

    pBAD

    Available Since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. XRCC1-c003

    Plasmid

    #173090

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    XRCC1 (XRCC1 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    E315-P406

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. myr-palm_mCherry-mEGFP

    Plasmid

    #127815

    Purpose

    Hetero-dimer mCherry-mEGFP expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

    Insert

    myristoylated-palmitoylated-mCherry-mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTXTL-T7max-LuxA

    Plasmid

    #188305

    Purpose

    Expression of LuxA via a T7 promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    luxA
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7Max

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. myc-Pex19

    Plasmid

    #127625

    Purpose

    N-terminal myc-tagged mouse Pex19

    Depositor

    Josef Kittler

    Article

    Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

    Insert

    Pex19 (Pex19 Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSBDEST.Z

    Plasmid

    #79470

    Purpose

    Promoterless, Sleeping Beauty-compatible Gateway destination vector, Zeocin

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Type

    Empty backbone

    Use

    Promoterless gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. EC2_7_dCas9_HC_sgRNA

    Plasmid

    #163711

    Purpose

    High copy plasmid with dCas9 and sgRNA expression cassettes

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Bongwoori-R3

    Plasmid

    #101658

    Purpose

    A variant of Bongwoori with H237R mutation in its linker region. (* super ecliptic pHluorin A227D)

    Depositor

    Bradley Baker

    Article

    Lee et al Sci Rep. 2017 Aug 15;7(1):8286. doi: 10.

    Insert

    Bongwoori-R3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET23a-Mm/HsUBA52

    Plasmid

    #69560

    Purpose

    Bacterial expression; in vitro transcription/translation (T7)

    Depositor

    Jorge Eduardo Azevedo

    Article

    Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

    Insert

    UBA52 (UBA52 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pKOdeltagalK (Kan)

    Plasmid

    #110089

    Purpose

    Analogous to pKO but without the negative selection gene galK.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Bacterial knockout generation

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFA6a-ytdTomato-CaURA3

    Plasmid

    #118461

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection. *An updated version of this plasmid is available: #168054 pFA6a-link-ytdTomato-URA3.

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ytdTomato
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MAC_C_STYK1

    Plasmid

    #187792

    Purpose

    MAC-tagged gene expression of human STYK1

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    STYK1 (STYK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAG-Ssh1

    Plasmid

    #163602

    Purpose

    Expresses Protein phosphatase Slingshot homolog 1 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    ssh1 (Ssh1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFA6a-ymVenus-CaURA3

    Plasmid

    #118459

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection. *An updated version of this plasmid is available: #168058 pFA6a-link-ymVenus-URA3.

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymVenus
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge