We narrowed to 15,037 results for: pET

1. pET-51b(+)-Caprola_14

   Plasmid

   #194675

   Purpose

   Protein production of the split HaloTag based calcium recorder Caprola_14 in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_14
   

   Use

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET-51b(+)-Caprola_15

   Plasmid

   #194676

   Purpose

   Protein production of the split HaloTag based calcium recorder Caprola_15 in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_15
   

   Use

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-pro-sumo3

   Plasmid

   #25103

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   pro-SUMO3 (SUMO3 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   changed Serine 2 to Alanine

   Promoter

   Available Since

   June 22, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pET28a(His10-PD-1cyto)

   Plasmid

   #177896

   Purpose

   E coli expression of His10 tag fused to human PD-1 Intracellular Domain

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   PD-1 (aa 194-288 only) (PDCD1 Human)
   

   Use

   Tags

   10xHis-Thrombin cut site-T7 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET14b PHAS-I F113A

   Plasmid

   #15680

   Depositor

   John Lawrence

   Article

   Choi et al J Biol Chem. 2003 May 30. 278(22):19667-

   Insert

   PHAS-I F113A (Eif4ebp1 Rat)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F113A mutation.

   Promoter

   Available Since

   Sept. 24, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pET28_6xHis-PARP1-Y986H

   Plasmid

   #174801

   Purpose

   Bacterial expression of a PARP1 mutant that produces highly branched but short PAR chains overall

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-Y986H (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Y986H, V762A

   Promoter

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28_6xHis-PARP1-Y986S

   Plasmid

   #174802

   Purpose

   Bacterial expression of a PARP1 mutant that produces mainly short PAR oligomers

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-Y986S (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Y986H, V762A

   Promoter

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28_6xHis-PARP1-G972R

   Plasmid

   #174800

   Purpose

   Bacterial expression of a PARP1 mutant that produces less PAR oligomers overall

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-G972R (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   G972R, V762A

   Promoter

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFS_0143-pET30-Fusicatenibacter_saccharivorans

   Plasmid

   #116952

   Purpose

   Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with classic acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Fusicatenibacter saccharivorans RT-Cas1-Cas2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-Hisx6-Sirt6

    Plasmid

    #20277

    Depositor

    Leonard Guarente

    Article

    Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

    Insert

    Sirt6 (Sirt6 Mouse)
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pET-PLS-KP6_rpoA-C

    Plasmid

    #190966

    Purpose

    Test the C-to-U editing activity of KP6 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET22b-T5-FB-E25TAG-F6C

    Plasmid

    #176546

    Purpose

    Expresses IgG affinity protein FB(F6C) with a TAG stop codon at E25 position

    Depositor

    Han Xiao

    Article

    Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

    Insert

    IgG affinity protein
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    change phenylalanine 6 to cysteine; change glutam…

    Promoter

    T5

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pETa(+) HpCsd3 (42-403)

    Plasmid

    #71285

    Purpose

    Expresses Csd3 from Helicobacter pylori

    Depositor

    SeWon Suh

    Article

    An et al Acta Crystallogr D Biol Crystallogr. 201

    Insert

    HP0506 (HP0506 )
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pET51b-NAD-cpLuc

    Plasmid

    #135628

    Purpose

    Bacterial expression of a biosensor for measuring NAD+ levels at the point of care (V1)

    Depositor

    Kai Johnsson

    Article

    Yu et al Nat Metab. 2019 Dec;1(12):1219-1225. doi

    Insert

    NAD-cpLuc sensor
    

    Use

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pETM-60-NusA-DmBelle_B

    Plasmid

    #145963

    Purpose

    Bacterial Expression of DmBelle

    Depositor

    Elisa Izaurralde

    Insert

    DmBelle (bel Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    del 155-157, T530A and I685V

    Promoter

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET16b-Trx C35S

    Plasmid

    #36947

    Depositor

    Anna Blom

    Article

    King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

    Insert

    Thioredoxin-1 C35S mutation (TXN Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    cys 35 to serine

    Promoter

    T7

    Available Since

    May 20, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pET30-hDlg PDZ2

    Plasmid

    #21692

    Depositor

    James Baleja

    Article

    Liu et al Biochemistry. 2007 Sep 25. 46(38):10864-

    Insert

    hDlg/SAP97 PDZ2 domain (DLG1 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    human hDlg/SAP97 PDZ2 domain (residues 318-405)

    Promoter

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pET-ChE1 v4.5

    Plasmid

    #185755

    Purpose

    Bacterial expression of ChE1 v4.5

    Depositor

    Jeffrey Bode

    Article

    Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

    Insert

    ChE1 v4.5
    

    Use

    Tags

    His10

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET SUMO-FL-syt1

    Plasmid

    #213628

    Purpose

    Bacterial expression of SUMO-tagged rat FL-syt1

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    FL-syt1 (Syt1 Rat)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-DUET-1-mRhubarb719-HO

    Plasmid

    #141200

    Purpose

    Expresses mRhubarb719 fluorescent protein and heme oxygenase

    Depositor

    Elad Firnberg

    Article

    Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

    Inserts

    mRhubarb719
    heme oxygenase
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    R134H, Y198S, T202Y, V203I, W309R, Q310A compared…

    Promoter

    T7

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Brg1 Del4 pET28C

    Plasmid

    #122118

    Purpose

    Expression Brg1 amino acids 1446-1647 in bacteria

    Depositor

    Paul Herring

    Article

    Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

    Insert

    Brg1 (SMARCA4 Human)
    

    Use

    Tags

    His-T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET19-M(BRCT)TdTwt

    Plasmid

    #207459

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domain

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    M(BRCT)TdTwt (Dntt Mouse)
    

    Use

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28-p53 (1-90) (72P)

    Plasmid

    #62080

    Purpose

    Expression of human p53 (1-90) (72P) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD/PP residues 1-90; 72P

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pET-DUET-Prp18

    Plasmid

    #111354

    Purpose

    protein purification

    Depositor

    Christine Guthrie

    Insert

    PRP18
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-His-Tre

    Plasmid

    #123129

    Purpose

    Bacterial expression plasmid for Tre with an N-terminal His-tag.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    Tre recombinase
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p3992 pET ubcH5

    Plasmid

    #8652

    Depositor

    Peter Howley

    Article

    Scheffner et al Proc Natl Acad Sci U S A 1994 Sep 13;91(

    Insert

    UbcH5 (UBE2D1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

27. HOXC13(human)-pET

    Plasmid

    #8679

    Depositor

    Corey Largman

    Article

    Komuves et al Dev Dyn 2003 Jun;227(2):192-202.

    Insert

    HOXC13 (HOXC13 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    makes protein by Coomassie blue staining

    Promoter

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

28. pETpRha-alpha-SkTm

    Plasmid

    #72929

    Purpose

    Allows expression of rabbit alpha-skeletal tropomyosin from a rhamnose inducible promoter

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

    Insert

    alpha skeletal Tropomyosin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhaB

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-2HMG-eGFP

    Plasmid

    #112737

    Purpose

    Express 2HMG-eGFP in E. coli

    Depositor

    Kyubong Jo

    Article

    Lee et al RSC Adv, 2016, 6, 46291-46298

    Insert

    2HMG-eGFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. DJ-1 TEV site pET15b

    Plasmid

    #60687

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    DJ-1 (PARK7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    TEV site was inserted (see comments below).

    Promoter

    T7

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only