We narrowed to 4,495 results for: Ado

1. pFETCh_KLF11

   Plasmid

   #86269

   Purpose

   Donor vector for 3' FLAG tag of human KLF11

   Depositor

   Richard M. Myers, Eric Mendenhall

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   KLF11 homology arms (KLF11 Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHL836

   Plasmid

   #62827

   Purpose

   Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::Asp terminator + PLtetO-1::RBS (st7) hfq::T1 terminator + ColE1

   Depositor

   Han Lim

   Article

   Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

   Inserts

   gfp
   ipex leader
   hfq leader
   

   Use

   Synthetic Biology

   Tags

   ompC leader and st7 RBS

   Expression

   Bacterial

   Mutation

   Promoter

   pLlacO-1 and pLtetO-1

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pHL1954

   Plasmid

   #62831

   Purpose

   Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::RBS (st7) hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1

   Depositor

   Han Lim

   Article

   Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

   Inserts

   M8::ryhB leader
   hfq leader
   gfp
   

   Use

   Synthetic Biology

   Tags

   m9::sodB leader and st7 RBS

   Expression

   Bacterial

   Mutation

   Promoter

   pConNoHind, pLlacO-1, and pLtetO-1

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pHL1969

   Plasmid

   #62832

   Purpose

   Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1

   Depositor

   Han Lim

   Article

   Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

   Inserts

   M8::ryhB leader
   T710 hfq leader
   gfp
   

   Use

   Synthetic Biology

   Tags

   m9::sodB leader

   Expression

   Bacterial

   Mutation

   Promoter

   pConNoHind, pLlacO-1, and pLtetO-1

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pHL2107

   Plasmid

   #62841

   Purpose

   Lim Lab plasmid: AmpR + PconNoHindM8::micC::Asp terminator + PLlacO-1::T710::hfq::T1 terminator + PLtetO-1m9::ompC::gfp::T1T2 terminator + ColE1

   Depositor

   Han Lim

   Article

   Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

   Inserts

   m8::micC leader
   T710 hfq leader
   gfp
   

   Use

   Synthetic Biology

   Tags

   m9::ompC leader

   Expression

   Bacterial

   Mutation

   Promoter

   pConNoHind, pLlacO-1, and pLtetO-1

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

6. Fuw-dCas9-Dnmt3a-P2A-tagBFP

   Plasmid

   #84569

   Purpose

   Lentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFP

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   dCas9-Dnmt3a (DNMT3A Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUCmini-iCAP-PHP.eB

   Plasmid

   #103005

   Purpose

   non-standard AAV2 rep-AAV-PHP.eB cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   Synthetic construct isolate AAV-PHP.eB VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. CROPseq-Guide-Puro

   Plasmid

   #86708

   Purpose

   Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.

   Depositor

   Christoph Bock

   Article

   Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n

   Insert

   EF1a-Puro-WPRE-hU6-gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHIV-EF1a-2A-Ovalbumin(dN)-ZsGreen

   Plasmid

   #231986

   Purpose

   Empty backbone for expression of Ovalbumin with Bi-cistronic expression ZsGreen

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   OVA-2A-ZsGreen
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUCmini-iCAP-PHP.S

    Plasmid

    #103006

    Purpose

    non-standard AAV2 rep-AAV-PHP.S cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    Synthetic construct isolate AAV-PHP.S VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. VPS13B^Halo

    Plasmid

    #224584

    Purpose

    codon optimized Halo tagged VPS13B for expression in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

    Insert

    VPS13B^Halo (VPS13B Human)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. P021-mPB-ENGRAM-All-in-one-PGK-BFP-P2A-Blast

    Plasmid

    #239972

    Purpose

    Updated ENGRAM plasmid that is ready for both 5' and 3'-FT ENGRAM cloning

    Depositor

    Jay Shendure

    Article

    Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pInducer20 TIFA WT

    Plasmid

    #231589

    Purpose

    TIFA WT gene under control of a doxycycline-inducible promoter

    Depositor

    Thomas Henry

    Article

    Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

    Insert

    TIFA (TIFA Human)
    

    Use

    Lentiviral

    Tags

    3X flag

    Expression

    Mammalian

    Mutation

    Promoter

    doxycycline

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. P022-mPB-TRE-PEmax-T2A-mCherry-PGK-rttA-Puro

    Plasmid

    #239974

    Purpose

    PiggyBac plasmid for inducible PEmax expression and Puromycin selection

    Depositor

    Jay Shendure

    Article

    Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.hSynapsin1.OxLight1

    Plasmid

    #169792

    Purpose

    Fluorescent reporter for orexin neuropeptides

    Depositor

    Tommaso Patriarchi

    Article

    Duffet et al Nat Methods. 2022 Feb;19(2):231-241. doi

    Insert

    OxLight1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin-1

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF1a-dCas9-KRAB-GFP backbone

    Plasmid

    #194281

    Purpose

    Vector for CRISPRi-GFP expression ready for gateway cloning of sgRNA

    Depositor

    Elizabeth Kirby

    Article

    Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

    Insert

    dCas9-KRAB-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRN3P_T3_ABE8e_IVT

    Plasmid

    #201676

    Purpose

    Plasmid to be used as DNA template for in vitro RNA transcription of the ABE8e base editor (A to G) by T3 RNA polymerase. The plasmid contains optimised 5'UTR and 3'UTR to improve protein expression.

    Depositor

    Timo Otonkoski

    Article

    Jalil et al Am J Hum Genet. 2024 Apr 4;111(4):714-72

    Insert

    ecTadA(8e)-nSpCas9
    

    Use

    CRISPR; Vector for in-vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T3 promoter

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC

    Plasmid

    #183790

    Purpose

    A single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.

    Depositor

    Jay Shendure

    Article

    Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

    Inserts

    P2A-eGFP-TargetBC-5xTAPE-1
    pegRNA-InsertBC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    G>A in the gRNA scaffold- please see depositor…

    Promoter

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-CRISPRv2-sgNTC13-hygro

    Plasmid

    #231988

    Purpose

    Knockout non-targeting control

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 and hygromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. EFS-SpdCas9

    Plasmid

    #210703

    Purpose

    This plasmid express EFS promoter driven human codon optimized SpdCas9

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    S. Pyogenes dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A/H840A

    Promoter

    EF1a core promoter

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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