Addgene

We narrowed to 4,968 results for: Apoe

Showing: 1421 - 1440 of 4968 results

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pHyg-yeGFP-His6

Plasmid

#212781

Purpose

C-terminal PCR-based tagging with yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-Flag-mMcl-1

Plasmid

#32982

Depositor

Joseph Opferman

Article

Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

Insert

Mcl-1 (Mcl1 Mouse)

Use

Retroviral

Tags

3XFlag

Expression

Mammalian

Mutation

Promoter

LTR

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
AAV-FLEX-rev-ChR2(H134R)-mCherry

Plasmid

#18916

Purpose

Cre-dependent viral expression of ChR2(H134R)-mCherry for neuronal excitation

Depositor

Scott Sternson

Article

Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

Insert

Channelrhodopsin 2-mCherry

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

mCherry

Expression

Mutation

H134R

Promoter

CAG

Available since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-IRES

Plasmid

#64784

Purpose

Luciferase reporter vector

Depositor

Joshua Mendell

Article

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Type

Empty backbone

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

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pWPI-TFDP1

Plasmid

#114299

Purpose

Constitutive expression of the TFDP1 protein

Depositor

Patrick Salmon

Article

Rady et al Cell Cycle. 2013 Aug 15;12(16):2691-702.

Insert

TFDP1 (TFDP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-TXNIP

Plasmid

#18758

Depositor

Clark Distelhorst

Article

Wang et al Oncogene. 2006 Mar 23. 25(13):1903-13.

Insert

thioredoxin-interacting protein (Txnip Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2008

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-Flag-mMcl-1 KR

Plasmid

#32983

Depositor

Joseph Opferman

Article

Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

Insert

Mcl-1 (Mcl1 Mouse)

Use

Retroviral

Tags

3XFlag

Expression

Mammalian

Mutation

All Lys to Arg

Promoter

LTR

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pCDH_Str-KDEL_neomycin

Plasmid

#65307

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end

Use

Lentiviral

Tags

KDEL motif and Signal Sequence

Expression

Mutation

Promoter

CMV

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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Ii-Str_TNF-SBP-EGFP

Plasmid

#65280

Purpose

synchronize trafficking of TNF from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Use

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-mCherry-GPI

Plasmid

#65295

Purpose

synchronize trafficking of mCherry-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Use

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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Tom20-CIB-GFP

Plasmid

#117242

Purpose

Light activated recruitment to OMM in mammalian cells with Cry2/CIB optogenetic switch

Depositor

Robert Hughes

Article

Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

Insert

CIBN

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMAL-c5x_UnaG

Plasmid

#163125

Purpose

A plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.

Depositor

Julie Biteen

Article

Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

Insert

UnaG

Use

Tags

His and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX 4T1 hBad

Plasmid

#35567

Depositor

Harish Srinivas

Article

Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

Insert

bad (BAD Human)

Use

Tags

Flag

Expression

Bacterial

Mutation

Promoter

Available since

March 26, 2012

Availability

Academic Institutions and Nonprofits only
pQC.Flip-nluc(LgBiT1-8)CP[TEV]

Plasmid

#200122

Purpose

Retroviral expression of Flip-nluc(LgBiT1-8)CP[TEV] protease reporter and T2A mCherry in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

Flip-nluc(LgBiT1-8)CP[TEV] T2A mCherry

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 iTEV

Plasmid

#64277

Purpose

Expresses infrared fluorescent tobacco etch virus protease sensor (iTEV)

Depositor

Xiaokun Shu

Article

To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

Infrared fluorescent tobacco etch virus protease sensor (iTEV)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pBT100.106b

Plasmid

#122601

Purpose

clone APOBEC1 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

APOBEC1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSH1/M-FRBl2-E

Plasmid

#15289

Depositor

David Spencer

Article

Li et al Gene Ther. 2002 Feb . 9(4):233-44.

Insert

FRAP-rapamycin binding domain (MTOR Human)

Use

Tags

HA epitope and Myristoylation-targeting domain c-…

Expression

Mammalian

Mutation

res. 2025-2113, T2098L

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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eGFP Y93H TC editing reporter

Plasmid

#198882

Purpose

Express eGFP fluorescence after Y93H correction and mCherry as transfection control

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

enhanced green fluorescent protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Y93H

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 CDK7 HA (P#633)

Plasmid

#14647

Depositor

Matija Peterlin

Article

Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

Insert

Cdk7 (CDK7 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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Ii-Str_ManII-SBP-mCherry

Plasmid

#65256

Purpose

synchronize trafficking of ManII from the ER

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ii-Streptavidin and truncated Mannosidase II

Use

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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