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Plasmid#221199PurposeScChd1-aa142-1274 chromatin remodeler with ChEx domain deletedDepositorInsertScChd1 (aa142-1274)
UseTags6xHis-tag (Precission protease cleavable)ExpressionBacterialMutationdeleted amino acids 1-117 and 1275-1468PromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST-3xFLAG-Ubc7
Plasmid#212801PurposeConstitutive expression of 3xFLAG-tagged budding yeast Ubc7 in mammalian cellsDepositorInsertUbc7
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDN0520 (pDEST, TRP1)
Plasmid#210481PurposepDEST vector with TRP1 marker for gene expression in S. cerevisiae.DepositorTypeEmpty backboneUseDestination vector for gateway cloningTagsExpressionMutationPromoterADH1Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA ins:TDimer mVenus
Plasmid#210520PurposeExpresses a tandem dimer of mVenus in zebrafish pancreatic beta cells using a zebrafish insulin promoterDepositorInsertTandem dimer mVenus
UseZebrafish expression, tol2 kit gateway-compatibleTagsExpressionMutationPromoterZebrafish insulin promoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA ins:R198P mVenus
Plasmid#210519PurposeExpresses mVenus-tagged R198P mutant of Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoterDepositorInsertApollo-NADP+
UseZebrafish expression, tol2 kit gateway-compatibleTagsmVenusExpressionMutationR198PPromoterZebrafish insulin promoterAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pATT-Dest-Cre
Plasmid#202714PurposeCre recombinase staging plasmid to make transposon insertion libraries.DepositorInsertCre
UseStaging plasmid to make transposon insertion libr…TagsExpressionMutationPromoterAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-HO1(51-231)
Plasmid#203486PurposeFor recombinant protein production in Escherichia coli. His-tagged Arabidopsis thaliana HEME OXYGENASE 1 (HO1) without the plastid transit peptide under the control of the T7 promoter.DepositorInsertArabidopsis thaliana HEME OXYGENASE1 (51 aa – 231 aa)
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti4/V5-DEST-AERRIE
Plasmid#204023PurposeLentiviral vector expressing human long non-coding RNA AERRIE (linc01013)DepositorInsertlong non-coding RNA AERRIE (LINC01013 Human)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3/TO/V5-DEST-mTurquoise2
Plasmid#191010PurposeLentiviral expression vector for mTurquoise2DepositorInsertmTurquoise2
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-Ctdnep1_C-ter
Plasmid#196525PurposeExpression of Ctdnep1_C-terDepositorInsertCtdnep1-C-ter (CTDNEP1 Human)
UseTagsHisExpressionBacterialMutationNo transmembrane domainPromoterT7 promoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pDEST40-CES2C-ΔC-S230A
Plasmid#200557PurposeMammalian expression of secreted mouse CES2C S230A mutantDepositorInsertMouse Carboxylesterase 2C S230A mutant delta C-terminal (Ces2c Mouse)
UseTags6xHis, Flag, and V5ExpressionMammalianMutationPromoterCMV promoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_1-183
Plasmid#196535PurposeExpression of GST-Eps8L2_1-183DepositorInsertEps8L2_1-183 (EPS8L2 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoter; lac UV5 promoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_181-299
Plasmid#196536PurposeExpression of GST-Eps8L2_181-299DepositorInsertEps8L2_181-299 (EPS8L2 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoter; lac UV5 promoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_544-715
Plasmid#196540PurposeExpression of GST-Eps8L2_544-715DepositorInsertEps8L2_544-715 (EPS8L2 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoter; lac UV5 promoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_297-370
Plasmid#196537PurposeExpression of GST-Eps8L2_297-370DepositorInsertEps8L2_297-370 (EPS8L2 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoter; lac UV5 promoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only