We narrowed to 2,911 results for: PPL

1. POC1478

   Plasmid

   #213804

   Purpose

   BsaI-associated switch methylase subunit S

   Depositor

   Christopher A. O'Callaghan

   Article

   Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

   Insert

   BsaI-associated switch methylase S.Sen0738I
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. POC1476

   Plasmid

   #213802

   Purpose

   BsaI-associated switch methylase subunit M

   Depositor

   Christopher A. O'Callaghan

   Article

   Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

   Insert

   BsaI-associated switch methylase M.Sen0738I
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. POC1477

   Plasmid

   #213803

   Purpose

   BsaI-associated switch methylase subunit S

   Depositor

   Christopher A. O'Callaghan

   Article

   Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

   Insert

   BsaI-associated switch methylase S.Sen0738I
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. POC1475

   Plasmid

   #213801

   Purpose

   BsaI-associated switch methylase subunit M

   Depositor

   Christopher A. O'Callaghan

   Article

   Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

   Insert

   BsaI-associated switch methylase M.Sen0738I
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMJ074

   Plasmid

   #215654

   Purpose

   pET28b derived T7 promoter with a 5’-UTR transcriptional fusion to clac143

   Depositor

   Brian Pfleger

   Article

   Jones et al Appl Microbiol Biotechnol. 2021 Feb;105(

   Insert

   Yellow fluorescent protein
   

   Use

   Synthetic Biology

   Tags

   10x histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSmart-EF1alpha-NlaCas7-p65

   Plasmid

   #207394

   Purpose

   Expresses NlaCas7-p65 activator fusion in mammalian cells

   Depositor

   Yan Zhang

   Article

   Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

   Insert

   EF1a-NlaCas7-p65-bGH polyA
   

   Use

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLP-sfgfp

   Plasmid

   #209994

   Purpose

   Contains Level 0 Part: Coding sequences (sfGFP) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher, Georg Fritz

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLPT1

   Plasmid

   #209995

   Purpose

   Contains Level 0 Part: Terminator (TB1006) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher, Georg Fritz

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   Terminator (TB1006)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLPS1

   Plasmid

   #209992

   Purpose

   Contains Level 0 Part: Ribosome binding site (RB0034) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher, Georg Fritz

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   RBS (RB0034)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLT237

    Plasmid

    #174300

    Purpose

    Replicating plasmid with homology region for introducing PolCC669Y mutation. Confers thiamphenicol resistance.

    Depositor

    Lee Lynd

    Article

    Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

    Insert

    PolC homology arm
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed Cysteine 669 to Tyrosine

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Gly-tRNA-gRNA scaffold for library 2

    Plasmid

    #192365

    Purpose

    tRNA sequence provider (for checkpoint library)

    Depositor

    Lijia Ma

    Article

    Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

    Insert

    Gly tRNA-gRNA scaffold
    

    Use

    For golden gate assembly

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Pro-tRNA-gRNA scaffold for library 2

    Plasmid

    #192366

    Purpose

    tRNA sequence provider (for checkpoint library)

    Depositor

    Lijia Ma

    Article

    Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

    Insert

    Pro tRNA-gRNA scaffold
    

    Use

    For golden gate assembly

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Gln-tRNA-gRNA scaffold for library 1&2

    Plasmid

    #192367

    Purpose

    tRNA sequence provider (for checkpoint and immune library)

    Depositor

    Lijia Ma

    Article

    Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

    Insert

    Gln tRNA-gRNA scaffold
    

    Use

    For golden gate assembly

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Gly-tRNA-gRNA scaffold for library 1

    Plasmid

    #192368

    Purpose

    tRNA sequence provider (for immune library)

    Depositor

    Lijia Ma

    Article

    Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

    Insert

    Gly tRNA-gRNA scaffold
    

    Use

    For golden gate assembly

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Pro-tRNA-gRNA scaffold for library 1

    Plasmid

    #192369

    Purpose

    tRNA sequence provider (for immune library)

    Depositor

    Lijia Ma

    Article

    Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

    Insert

    Pro tRNA-gRNA scaffold
    

    Use

    For golden gate assembly

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPpT4- HTA1prom-TIR1-FLAG

    Plasmid

    #189725

    Purpose

    Enables expression of OsTIR1 from the HTA1 promoter, used to implement the AID system in Komagataella phaffii (original name pLLpLL001)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    HTA1

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPpT4- TEF2prom-TIR1-FLAG

    Plasmid

    #189726

    Purpose

    Enables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    TEF2

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NAX_CAGGS_CAS9_VPR_mcherry

    Plasmid

    #167894

    Purpose

    PiggyBac compatible plasmid expressing Cas9-VPR

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

19. NAX_CAGGS_CAS9_m4_mcherry

    Plasmid

    #167870

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

20. pET3a-FruK

    Plasmid

    #186256

    Purpose

    Overexpression of FruK for purification

    Depositor

    Emile Van Schaftingen, Liskin Swint-Kruse

    Article

    Veiga-da-Cunha et al Protein Expr Purif. 2000 Jun;19(1):48-52

    Insert

    1-phosphofructokinase (fruK E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge