We narrowed to 10,113 results for: ada

1. FLAG-HA-FbxO11-ΔFbox-pcDNA3.1-

   Plasmid

   #52502

   Purpose

   expresses human FbxO11-ΔFbox in mammalian cells with FLAG-HA tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   FbxO11 (FBXO11 Human)
   

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   deletion of Fbox (deleted aa 71-116)

   Promoter

   CMV

   Available Since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMIG-FLAG-MLL-AF9

   Plasmid

   #71443

   Purpose

   Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-GFP

   Depositor

   Daisuke Nakada

   Article

   Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

   Insert

   MLL-Af9 (KMT2A Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   MSCV

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. PEmax_AAVS1_knockin_HDR_donor

   Plasmid

   #223000

   Purpose

   HDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PEmax
   

   Use

   Hdr donor

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. miABE

   Plasmid

   #205412

   Purpose

   Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

   Insert

   pU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
   

   Expression

   Mammalian

   Mutation

   D61A+E85R+H369R+S387R+S457R

   Promoter

   hU6, CAG, CMV

   Available Since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3xFlag-eGFP-Flag-SETX

   Plasmid

   #218838

   Purpose

   Mammalian expression of GFP-tagged SETX construct for subcellular localization studies with GFP, or immunoprecipitation with Flag tag.

   Depositor

   Albert La Spada

   Article

   Bennett et al Heliyon. 2020 Jun 13;6(6):e04165. doi: 1

   Insert

   Senataxin (SETX Human)
   

   Tags

   Flag and eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. α‐eGFP

   Plasmid

   #160214

   Purpose

   Retroviral construct to express an eGFP-tagged version of the α subunit of AP2

   Depositor

   Sandra Schmid

   Article

   Mino et al Traffic. 2020 Sep;21(9):603-616. doi: 10

   Insert

   α‐eGFP (Ap2a2, Ap2a1 Mouse)
   

   Use

   Retroviral

   Tags

   eGFP at Hinge region

   Mutation

   Brain-specific Insert

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMIR-FLAG-MLL-AF9

   Plasmid

   #71444

   Purpose

   Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-dsRed Express2

   Depositor

   Daisuke Nakada

   Article

   Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

   Insert

   MLL-AF9 (KMT2A Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   MSCV

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. loxP-G418-LoxP-TurboID-Rab11 HR

   Plasmid

   #230026

   Purpose

   Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells

   Depositor

   Francesca Bottanelli

   Article

   Adarska et al STAR Protoc. 2025 Aug 4;6(3):103949. doi

   Insert

   genomic sequence Rab11 locus (RAB11A Human)
   

   Use

   CRISPR

   Tags

   Turbo-ID, V5

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. CamKII-LGI1-pHluorin

   Plasmid

   #185537

   Purpose

   Expresses LGI1-pHluorin under the CamKII promoter

   Depositor

   Jaime de Juan-Sanz

   Article

   Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

   Insert

   LGI1 (Lgi1 Rat)
   

   Use

   Lentiviral

   Tags

   pHluorin

   Expression

   Mammalian

   Promoter

   CamKII

   Available Since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. CamKII-LGI1-pHmScarlet

    Plasmid

    #185538

    Purpose

    Expresses LGI1-pHmScarlet under the CamKII promoter

    Depositor

    Jaime de Juan-Sanz

    Article

    Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

    Insert

    LGI1 (Lgi1 Rat)
    

    Use

    Lentiviral

    Tags

    pHmScarlet

    Expression

    Mammalian

    Promoter

    CamKII

    Available Since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNIC-ZB-SPRTNwt-FL

    Plasmid

    #110217

    Purpose

    Expression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.

    Depositor

    Kristijan Ramadan

    Article

    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

    Insert

    SPRTN (SPRTN Human)
    

    Expression

    Bacterial

    Mutation

    P296L (see depositor comments below)

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. SPACE (pRZ1813)

    Plasmid

    #140242

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE
    

    Expression

    Mammalian

    Mutation

    V82G in TadA*, D10A in Cas9, R187W in pmCDA1

    Promoter

    CMV

    Available Since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. DENV4 Rluc Reporter GND mutant Replicon

    Plasmid

    #118586

    Purpose

    For use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.

    Depositor

    Radhakrishnan Padmanabhan

    Article

    Alcaraz-Estrada et al J Gen Virol. 2010 Nov;91(Pt 11):2713-8.

    Insert

    DENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
    

    Use

    Yeast-e. col shuttle vector prs424

    Mutation

    The conserved GDD in the NS5 polymerase gene muta…

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. SPACE-NG (pRZ5150)

    Plasmid

    #140244

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE_NG
    

    Expression

    Mammalian

    Mutation

    V82G in TadA*, NG mutations in SpCas9, D10A in Sp…

    Promoter

    CMV

    Available Since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOD2046-bwmDEG

    Plasmid

    #89367

    Purpose

    MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmyo-3 (body wall muscle specific).

    Depositor

    Karen Oegema

    Article

    Wang et al Development. 2017 Jun 15. pii: dev.15009

    Insert

    vhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
    

    Use

    Targeting vector for mos1 transposon mediated sin…

    Tags

    vhhGFP4 (GFP nanobody)

    Promoter

    Pmyo-3

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. NST1_pOpOn2.1

    Plasmid

    #228065

    Purpose

    Expresses NST1 in different cell types in Arabidopsis thaliana upon induction with dexamethasone.

    Depositor

    Charles Anderson

    Article

    Tamadaddi et al Ann Bot. 2024 Aug 30:mcae153. doi: 10.10

    Insert

    NAC SECONDARY WALL THICKENING PROMOTING FACTOR1, NST1 (NST1 Mustard Weed)
    

    Use

    Dexamethasone inducible vector

    Expression

    Plant

    Promoter

    CaMV35S for LhGR and pOp6-minimal CaMV35S for NST1

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOD2048-csnDEG

    Plasmid

    #89368

    Purpose

    MosSCI targeting vector expressing a fusion between a GFP nanobody and ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression controlled by Posm-6 (ciliated sensory neuron specific)

    Depositor

    Karen Oegema

    Article

    Wang et al Development. 2017 Jun 15. pii: dev.15009

    Insert

    vhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
    

    Use

    Targeting vector for mos1 transposon mediated sin…

    Tags

    vhhGFP4 (GFP nanobody)

    Promoter

    Posm-6

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

18. SPACE-VRQR (pRZ5137)

    Plasmid

    #140245

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9_VRQR-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE_VRQR
    

    Expression

    Mammalian

    Mutation

    V82G in TadA*, VRQR mutations in SpCas9, D10A in …

    Promoter

    CMV

    Available Since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. SPACE-ΔUGI (pRZ1836)

    Plasmid

    #140243

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE-ΔUGI
    

    Expression

    Mammalian

    Mutation

    V82G in TadA*, D10A in Cas9, R187W in pmCDA1

    Promoter

    CMV

    Available Since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. CRY2clust-mCherry-RANK-P2A-CIBNcaax

    Plasmid

    #208613

    Purpose

    Mammalian expression of CRY2clust-mCherry-RANK-P2A-CIBNcaax (Opto-RANKm)

    Depositor

    Tomohiro Ishii, Takao Nakata

    Article

    Takada et al Sci Rep. 2024 Jan 19;14(1):1749. doi: 10

    Insert

    CRY2clust-mCherry-RANK-P2A-CIBNcaax (Cry2 Mouse, Mustard Weed)
    

    Tags

    CAAX and mCherry

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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