We narrowed to 8,856 results for: Plat

1. pDL17_Cas9-His Δcys M1C

   Plasmid

   #206251

   Purpose

   Bacterial expression of SpCas9 Δcys M1C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   M1C, C80S, C574S

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCK01_PalkS_alkS

   Plasmid

   #166502

   Purpose

   Expression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.

   Depositor

   Sven Panke

   Article

   Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2

   Insert

   alkS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   L401G

   Promoter

   PalkS

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC0075 TtCsm6 His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115270

   Purpose

   Expresses TtCsm6 from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   TtCsm6
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-hSyn-Galpha-q ONE-GO

   Plasmid

   #204336

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   hSyn Galpha-q ONE-GO
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. NmCas9_sgRNA_expression_in_pBluescript

   Plasmid

   #122091

   Purpose

   U6 driven NmCas9 sgRNA expression vector for cloning own guides

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   NmCas9 tracrRNA (NEWENTRY Synthetic, S. pyogenes)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMaCTag-P22

   Plasmid

   #120033

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with SNAP

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   SNAP
   

   Use

   Pcr template

   Tags

   SNAP

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2_Renilla_luc-60A

   Plasmid

   #200397

   Purpose

   A plasmid IVT template containing a Renilla luciferase CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

   Depositor

   Xiao Wang

   Article

   Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

   Insert

   Renilla luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLZRS-IRES-deltaNGFR

   Plasmid

   #72930

   Purpose

   retroviral expression with FACS-selectable marker (extracellular part of NGFR)

   Depositor

   Esther van de Vosse

   Article

   van_de_Vosse et al Blood. 2009 May 7;113(19):4548-55. doi:

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSETA-qmTQ2-citrate

   Plasmid

   #225251

   Purpose

   Bacterial expression of N-terminal His-tagged qmTQ2-citrate, a cyan fluorescent FLIM biosensor for detecting citrate

   Depositor

   Yasushi Okada

   Article

   Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

   Insert

   qmTQ2-citrate
   

   Use

   Tags

   6XHis, T7 tag, XpressTM tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-mas-KB2123-Nluc

    Plasmid

    #204325

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    KB2123-Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. MXS_CMV::PuroR-bGHpA

    Plasmid

    #62439

    Purpose

    MXS_chaining vector with CMV::PuroR-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    resistance cassette against Puromycin with CMV Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 5HT2A_NB2_1

    Plasmid

    #127690

    Purpose

    Plasmid contains a positive allosteric modulating nanobody the binds to the cytoplasmic surface of 5HT2A.

    Depositor

    Justin English, Bryan Roth

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    5HT2A_NB2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSb_init containing anti SARS-CoV-2_RBD sybody Sb#68

    Plasmid

    #153527

    Purpose

    Expression of the anti SARS-CoV-2_RBD sybody Sb#68

    Depositor

    Markus Seeger

    Article

    Walter et al bioRxiv 2020.04.16.045419

    Insert

    Sb#68
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTREX GPR68_NB2_2

    Plasmid

    #127691

    Purpose

    Plasmid contains a nanobody that directly interacts with the cytoplasmic surface of GPR68.

    Depositor

    Justin English, Bryan Roth

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    GPR68_NB2_2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLVX-hSyn-Galpha-i3 ONE-GO

    Plasmid

    #204334

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    hSyn Galpha-i3 ONE-GO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pMaCTag-P33

    Plasmid

    #120018

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with FLAG

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    FLAG
    

    Use

    Pcr template

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. IZ501: pMVP/Lenti-DEST

    Plasmid

    #121848

    Purpose

    pMVP destination vector, empty Lentivirus vector backbone

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Lentiviral; Pmvp gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMaCTag-P26

    Plasmid

    #120037

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mAID-Clover

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mAID-Clover
    

    Use

    Pcr template

    Tags

    mAID-Clover

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. His-DERA

    Plasmid

    #203225

    Purpose

    For protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

    Depositor

    Vern Schramm

    Article

    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

    Insert

    deoxyribose aldolase
    

    Use

    Tags

    N-terminal TEV cleavable 6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMaCTag-P27

    Plasmid

    #120038

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 1xHA

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    1xHA
    

    Use

    Pcr template

    Tags

    1xHA

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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