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ECFP-Bak

Plasmid

#31501

Depositor

Richard Youle

Article

Nechushtan et al J Cell Biol. 2001 Jun 11. 153(6):1265-76

Insert

human Bak (BAK1 Human)

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA-Sirius-4X(MS2-PP7)

Plasmid

#121941

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-4X(MS2-PP7)

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

human U6 promoter

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-rat Nrxn1beta AS4(-)

Plasmid

#58267

Purpose

Expression plasmid for HA-tagged rat Neurexin 1beta, lacking the insert at alternatively spliced segment 4 (AS4-)

Depositor

Peter Scheiffele

Article

Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Insert

Nrxn1beta AS4(-) (Nrxn1 Rat)

Tags

HA

Expression

Mammalian

Mutation

see comments

Promoter

CAG

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pTP728

Plasmid

#216374

Purpose

xNup358-Nb1t (+3Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xNup358-Nb1t (+3Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2]

Plasmid

#74101

Purpose

vector bearing an NheI restriction site for the two-step cloning method in Wang and Szaro, Dev Biol, 2015

Depositor

Ben Szaro

Article

Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

Inserts

Green Lantern Green Fluorescent Protein
Rabbit beta 1-globin gene 3'UTR

Available Since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 CDK8 HA (P#634)

Plasmid

#14649

Depositor

Matija Peterlin

Article

Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

Insert

Cdk8 (CDK8 Human)

Tags

HA

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pDG04439

Plasmid

#216365

Purpose

xhNup133-Nb2t (+2Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup133-Nb2t (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCVpuroATT

Plasmid

#47539

Purpose

Retroviral Gateway destination vector constructed by inserting Reading Frame Cassette A into pMSCVpuro (Clontech) using the Gateway Vector Conversion System (Invitrogen)

Depositor

David Weinstock

Article

Shindoh et al PLoS One. 2012;7(11):e49201. doi: 10.137

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only
pKD4-Nter-APEX2

Plasmid

#112868

Purpose

Bacterial plasmid derived from pKD4 for chromosomal insertion of APEX2 gene in 5' of a gene of interest by using Datsenko & Wanner method

Depositor

Eric Cascales

Article

Santin et al Nat Microbiol. 2018 Nov;3(11):1304-1313.

Insert

APEX2

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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WT lamin A minigene

Plasmid

#20291

Depositor

Tom Misteli

Article

Scaffidi et al Nat Med. 2005 Apr . 11(4):440-5.

Insert

lamin A (LMNA Human)

Tags

GFP

Expression

Mammalian

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pIntegrase_3

Plasmid

#60575

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_3

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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rs214-minor

Plasmid

#113380

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs2149943 minor allele (T)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs2149943 minor allele

Use

Luciferase

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pEasy Flox

Plasmid

#11725

Depositor

Klaus Rajewsky

Article

Schenten et al Eur J Immunol. 2002 Nov . 32(11):3152-60

Insert

pEasyFlox

Use

Cre/Lox

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pNAS1B

Plasmid

#61968

Purpose

Duet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectors

Depositor

Lynne Regan

Article

Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

Inserts

mCherry
GFP

Expression

Bacterial

Mutation

Serine 65 to Glycine (GGT), Serine 72 to Alanine …

Promoter

PBAD and PLtetO

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pMSC226

Plasmid

#216375

Purpose

xhNup358-Nb2t (+3Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup358-Nb2t (+3Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5

Plasmid

#63168

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-V5"

Tags

N-myristoylation

Expression

Insect

Promoter

GAL4-responsive UAS hsp70

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pReporter_3

Plasmid

#60564

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_3

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pReporter_4

Plasmid

#60565

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_4

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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ER50-SpCas9-ER50

Plasmid

#85448

Purpose

Expresses SpCas9 fused to ER50 domain on both N- and C-termini in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

ER50-SpCas9-ER50

Use

AAV

Tags

destabilized domain of estrogen receptor ligand b…

Expression

Mammalian

Promoter

Cbh

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCFJ910

Plasmid

#44481

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, NeoR, MCS

Use

Multiple cloning site vector

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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DYSF-Venus

Plasmid

#29768

Depositor

Steven Vogel

Article

Covian-Nares et al J Cell Sci. 2010 Jun 1. 123(Pt 11):1884-

Insert

DYSF-Venus (DYSF Human)

Tags

Venus

Expression

Mammalian

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
pBS1479-HTP-KanMX6

Plasmid

#154085

Purpose

For C-terminal his6-TEV-ProtA-tagging in S. pombe using KanMX6 as resistance marker.

Depositor

Lidia Vasiljeva

Article

Kilchert et al Cell Rep. 2015 Dec 22;13(11):2504-2515.

Insert

his6-TEV-protein A-KanR

Use

Pcr-based tagging in yeast

Tags

his6-TEV-protein A

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBN1

Plasmid

#86710

Purpose

C. elegans injection marker

Depositor

Peter Askjaer

Article

Rodenas et al Mol Biol Cell. 2012 Mar;23(5):930-44. do

Insert

Plmn-1::mCherry::his-58::pie-1 3'UTR

Expression

Worm

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pJFRC203-10XUAS-FRT>STOP>FRT-myr::smGFP-cMyc

Plasmid

#63167

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-cMyc reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-cMyc"

Tags

N-myristoylation

Expression

Insect

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-R4-uAb

Plasmid

#101800

Purpose

Mammalian cell expression of the beta-galactosidase-specific ubiquibody, R4-uAb

Depositor

Matthew DeLisa

Article

Portnoff et al J Biol Chem. 2014 Mar 14;289(11):7844-55

Insert

R4-uAb

Tags

6xHis and Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-C2gamma

Plasmid

#21215

Depositor

Tobias Meyer

Article

Codazzi et al Curr Biol. 2001 Jul 24. 11(14):1089-97.

Insert

GFP-pcDNA3-PKCgamma-c2 (Prkcg Rat)

Tags

GFP

Expression

Mammalian

Mutation

The C2-domain from PKCgamma (aa170-260) plus flan…

Available Since

July 13, 2009

Availability

Academic Institutions and Nonprofits only

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RhoC FLARE.sc mCer, mVenus - wt

Plasmid

#65071

Purpose

RhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC

Depositor

Klaus Hahn

Article

Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

Insert

RBD, mCerulean, mVenus, RhoC

Expression

Bacterial, Insect, and Mamm…

Mutation

T153M in mVenus

Promoter

CMV, p10

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only
pReporter_12

Plasmid

#60572

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_12

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pRosa26 R1-ccdB-R2 RexNeo PI-SceI

Plasmid

#24418

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

Use

Mouse Targeting

Available Since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

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pCAG-3xGFP11-mem-3xControlgRNA

Plasmid

#224583

Purpose

Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

3xGFP11

Use

CRISPR

Tags

Membrane Localization Signal

Mutation

Codon optimized

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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