We narrowed to 21,976 results for: his

1. pBJG1 T7-8xHis-OGT D554N

   Plasmid

   #154279

   Purpose

   Bacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

   Depositor

   Suzanne Walker

   Article

   Janetzko et al Nat Chem Biol. 2016 Nov;12(11):899-901.

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Tags

   8x His, HRV3C protease site, and T7 leader

   Expression

   Bacterial

   Mutation

   Aspartate 554 changed to Asparagine- bacterial co…

   Promoter

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFL His-SDS22 / PP1gamma

   Plasmid

   #113511

   Purpose

   Expression of His-SDS22 / PP1gamma dimer in insect cells (Baculovirus system)

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Inserts

   SDS22 (PPP1R7 Human)
   PP1gamma (PPP1CC Human)
   

   Use

   Tags

   6*His

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET22b-mEB3-mCherry-6xHis

   Plasmid

   #130985

   Purpose

   Bacterial expression of mouse EB3-mCherry-6xHis for protein purification.

   Depositor

   Anne Straube

   Article

   Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

   Insert

   EB3-mCherry-6xHis (Mapre3 Mouse)
   

   Use

   Tags

   mCherry-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHIS3p:mRuby2-Tub1+3'UTR::URA3

   Plasmid

   #50639

   Depositor

   Wei-Lih Lee

   Article

   Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

   Insert

   mRuby2-Tub1
   

   Use

   Yeast expression

   Tags

   mRuby2

   Expression

   Mutation

   Promoter

   HIS3p

   Available since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA4-TO-myc-His wt-Trask

   Plasmid

   #31768

   Depositor

   Mark Moasser

   Article

   Spassov et al PLoS One. 2011 Apr 29;6(4):e19154.

   Insert

   Trask (CDCP1 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET30a-His6-ABP-LOXCATmut

   Plasmid

   #139972

   Purpose

   A control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

   Depositor

   Vamsi Mootha

   Article

   Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

   Insert

   A fusion of lactate oxidase from A. viridans and catalase from E. coli
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Mutation in H265A and R268A in LOX (used original…

   Promoter

   T7

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21a(+)-Histag-Cerulean (526)

   Plasmid

   #70716

   Purpose

   Expression vector for Cerulean purification

   Depositor

   Richard Murray

   Article

   Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

   Insert

   Cerulean
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSL0374 (His-MBP-Cas8_Cas7_Cas6)

   Plasmid

   #130639

   Purpose

   Expresses V. cholerae CAST Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on Cas8 for purification.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchCas8, VchCas7, VchCas6
   

   Use

   CRISPR; Transposon

   Tags

   His10-MBP-TEVsite on Cas8

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a(+) 6xHis-TEV-IFIT3

   Plasmid

   #53559

   Purpose

   bacterial expression of IFIT3

   Depositor

   Kathleen Collins

   Article

   Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

   Insert

   IFIT3 (IFIT3 Human)
   

   Use

   Tags

   6xHIS and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pHybr_r2a>mG2a-srt-his

    Plasmid

    #124803

    Purpose

    HDR template to knock-in mouse IgG2a Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2a producing cell lines.

    Depositor

    Martijn Verdoes, Ferenc Scheeren

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    Murine IgG2a
    

    Use

    CRISPR; Hdr template

    Tags

    Histag (HHHHHH) and Sortag (LPETGG)

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEB31 (hANGPTL8-V5-His lenti)

    Plasmid

    #109116

    Purpose

    V5 and His-tagged human ANGPTL8 in a lentiviral vector

    Depositor

    Brandon Davies

    Article

    Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

    Insert

    ANGPTL8 (ANGPTL8 Human)
    

    Use

    Lentiviral

    Tags

    6x His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFRT/TO/HIS/FLAG/HA-YTHDF2

    Plasmid

    #38089

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    YTHDF2 YTH domain family, member 2 (YTHDF2 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. YEp195-CUP1-His-Smt3

    Plasmid

    #99538

    Purpose

    Yeast episomal vector for expression of His-tagged Smt3 (SUMO); URA3 marker

    Depositor

    Helle Ulrich

    Article

    Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

    Insert

    SMT3 (SMT3 Budding Yeast)
    

    Use

    Tags

    7xHis

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR-HLA-B0702-His

    Plasmid

    #104849

    Purpose

    HLA-B0702 his tagged

    Depositor

    Sébastien Walchli

    Article

    Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

    Insert

    major histocompatibility complex, class I, B (HLA-B Human)
    

    Use

    Entry vector for gateway system

    Tags

    his

    Expression

    Mutation

    Promoter

    none

    Available since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. MBP-His6-TEV-MMS2

    Plasmid

    #25465

    Depositor

    Cynthia Wolberger

    Article

    Eddins et al Nat Struct Mol Biol. 2006 Oct . 13(10):9

    Insert

    MMS2 (MMS2, UBC13 Budding Yeast)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pFRT/TO/HIS/FLAG/HA-PES1

    Plasmid

    #38085

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    PES1 pescadillo ribosomal biogenesis factor 1 (PES1 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET51b-His-TEV-HaloTag7_dead_mt

    Plasmid

    #167267

    Purpose

    Overexpression of the dead variant (D106A) of HaloTag7 in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    HaloTag7
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    D106A

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFRT/TO/HIS/FLAG/HA-BTF3

    Plasmid

    #38075

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    BTF3 basic transcription factor 3 (BTF3 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p2CT-His-MBP-Lbu_C2c2_R1079A

    Plasmid

    #83494

    Purpose

    Bacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_R1079A
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    R1079A

    Promoter

    T7

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAc5.1B-lambdaN-HA-DmeIF4G-V5His6

    Plasmid

    #79259

    Purpose

    Expresses lambdaN-HA-tagged and C-terminal V5-His6-tagged DmeIF4G in S2 cells

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al EMBO J. 2013 Apr 3;32(7):1052-65. doi: 1

    Insert

    DmeIF4G (eIF4G1 Fly)
    

    Use

    Schneider 2 cell expression

    Tags

    V5-His6 and lambdaN-HA

    Expression

    Mutation

    Promoter

    Ac5 promoter

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28b-LanCL2 (6xHis-LanCL2)

    Plasmid

    #73407

    Purpose

    Expresses His tagged LanCL2

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    LanCL2 (LANCL2 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

22. pGEX4T1-GST-eIF4G1-His6

    Plasmid

    #37231

    Depositor

    Jon Lorsch

    Article

    Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

    Insert

    TIF4631 (TIF4631 Budding Yeast)
    

    Use

    Tags

    GST and His6

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

23. pAS1-rab-3P::his-24::CyOFP

    Plasmid

    #124347

    Purpose

    Nuclear Localized Pan-Neuronal CyOFP

    Depositor

    Andrew Leifer

    Insert

    rab-3, CyOFP (rab-3 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28a(+) 6xHis-TEV-IFIT2

    Plasmid

    #53558

    Purpose

    bacterial expression of IFIT2

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT2 (IFIT2 Human)
    

    Use

    Tags

    6xHIS and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pcDNA4/TO/myc-His-A-YAP2

    Plasmid

    #19060

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Jul 17. ():.

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

26. pET-DEST42-ARF6-V5-His6

    Plasmid

    #67415

    Purpose

    Expression of hArf6 with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF6 (ARF6 Human)
    

    Use

    Tags

    His and V5

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lacO

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pRPL28(K55M) SpHIS5 (pNTI666)

    Plasmid

    #115436

    Purpose

    Expresses K55M RPL28 from HIS3 yeast plasmid

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    K55M; MfeI site in intron

    Promoter

    Ashbya gospii TEF1 and endogenous

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFA6a-link-yoGFPgamma-SpHis5

    Plasmid

    #44859

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized GFPgamma with SpHis5 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoGFPgamma
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBOB-CAG-fHis-H2A-Ub

    Plasmid

    #41797

    Depositor

    Inder Verma

    Article

    Zhu et al Nature. 2011 Sep 7;477(7363):179-84. doi

    Insert

    histone H2A
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET21a-Med25-VBD-6His

    Plasmid

    #64773

    Purpose

    Bacterial expression of His-tagged Med25-VBD

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    Med25-VBD (MED25 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    insert is 394-543 only

    Promoter

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only