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We narrowed to 6,530 results for: nls

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Showing: 2881 - 2920 of 6530 results

  7. pcDNA3-nuc-Nb80

    Plasmid
    #205519
    Purpose
    Nuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.
    Depositor
    Jin Zhang
    Article
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    nuc-Nb80
    Tags
    Nuclear localization signal (NLS) and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205519-plasmid-map-sequence-id-409422

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  16. DCIP

    Plasmid
    #193860
    Purpose
    Encodes SV40-mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.
    Depositor
    Markita Landry
    Article
    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:
    Insert
    NLS-mCherry-GFP1-10
    Tags
    SV40 NLS
    Expression
    Plant
    Promoter
    35S
    Available Since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    193860-plasmid-map-sequence-id-391139

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  20. MSP2283

    Plasmid
    #70702
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70702-plasmid-map-sequence-id-127769

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  22. OmEF1aCas9P2ApuroSB

    Plasmid
    #165484
    Purpose
    Stable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells
    Depositor
    Dietmar Kueltz
    Article
    Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10
    Insert
    Zebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)
    Use
    CRISPR; Transposon
    Promoter
    Oreochromis mossambicus EF1 alpha promoter (OmEF1…
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    165484-plasmid-map-sequence-id-330622

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  24. A3A-PBE-ΔUGI

    Plasmid
    #119770
    Purpose
    Conversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)
    Depositor
    Caixia Gao
    Article
    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426
    Insert
    APOBEC3A-nCas9-ΔUGI-NLS
    Tags
    NLS
    Expression
    Plant
    Mutation
    nCas9 has a mutation D10A in wild type SpCas9
    Promoter
    maize Ubiquitin-1
    Available Since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119770-plasmid-map-sequence-id-232089

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  26. pNS3

    Plasmid
    #131770
    Purpose
    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites
    Depositor
    Jared Toettcher
    Article
    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d
    Insert
    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1
    Use
    Integration into δ-sites
    Expression
    Yeast
    Mutation
    E490G to make Cry2 into Cry2Olig
    Promoter
    PADH1
    Available Since
    Sept. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    131770-plasmid-map-sequence-id-257191

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  28. MSP2266

    Plasmid
    #70705
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70705-plasmid-map-sequence-id-131909

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Showing: 2881 - 2920 of 6530 results