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Showing: 3601 - 3650 of 18941 results

  6. pJK372

    Plasmid
    #73597
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) with C-terminal c-myc epitope
    Depositor
    T. J. Kappock
    Inserts
    delta-12 fatty acid acetylenase
    delta-15 fatty acid desaturase
    Use
    Tags
    FLAG tag and c-myc tag
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73597-plasmid-map-sequence-id-138754

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  7. pJK370

    Plasmid
    #73595
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitope
    Depositor
    T. J. Kappock
    Inserts
    delta-12 fatty acid acetylenase
    delta-12 fatty acid desaturase (form 2)
    Use
    Tags
    FLAG tag and c-myc tag
    Expression
    Yeast
    Mutation
    Silent mutation in Arg299 codon.
    Promoter
    GAL1 and GAL10
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    73595-plasmid-map-sequence-id-138462

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  8. pDYU1H-TED

    Plasmid
    #154300
    Purpose
    A expression vector in Dictyostelium discoideum containing the Hygromycin B resistant gene and the TED module for GFP-negative screening
    Depositor
    Shigehiko Yumura
    Article
    Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi
    Type
    Empty backbone
    Use
    Dictyostelium expression
    Tags
    Expression
    Mutation
    Promoter
    actin15
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    154300-plasmid-map-sequence-id-316748

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  9. pDYU1B-TED

    Plasmid
    #154299
    Purpose
    A expression vector in Dictyostelium discoideum containing the Blasticidin S resistant gene and the TED module for GFP-negative screening
    Depositor
    Shigehiko Yumura
    Article
    Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi
    Type
    Empty backbone
    Use
    Dictyostelium expression
    Tags
    Expression
    Mutation
    Promoter
    actin15
    Available since
    Jan. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    154299-plasmid-map-sequence-id-307553

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  15. pDYU1G-TED

    Plasmid
    #154298
    Purpose
    A expression vector in Dictyostelium discoideum containing the G418 resistant gene and the TED module for GFP-negative screening
    Depositor
    Shigehiko Yumura
    Article
    Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi
    Type
    Empty backbone
    Use
    Dictyostelium expression
    Tags
    Expression
    Mutation
    Promoter
    actin15
    Available since
    Jan. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    154298-plasmid-map-sequence-id-307552

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  19. pYHY3

    Plasmid
    #216109
    Purpose
    aTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.
    Depositor
    Shannon Sirk
    Article
    Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:
    Insert
    NanoLuc
    Use
    Synthetic Biology; E. coli-bacteroides shuttle ve…
    Tags
    3xFLAG tag and BT3630 signal peptide
    Expression
    Bacterial
    Mutation
    Promoter
    P1TDP-A21
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216109-plasmid-map-sequence-id-434372

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  23. pYHY2

    Plasmid
    #216108
    Purpose
    Constitutive expression of secretory BT0294 SP-NanoLuc via BfP1E6-RBS8 promoter-RBS pair in Bacteroides species.
    Depositor
    Shannon Sirk
    Article
    Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:
    Insert
    NanoLuc
    Use
    Synthetic Biology; E. coli-bacteroides shuttle ve…
    Tags
    3xFLAG tag and BT0294 signal peptide
    Expression
    Bacterial
    Mutation
    Promoter
    BfP1E6 promoter
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216108-plasmid-map-sequence-id-434369

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  44. pJK371

    Plasmid
    #73596
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) with C-terminal c-myc epitope
    Depositor
    T. J. Kappock
    Inserts
    delta-12 fatty acid acetylenase
    delta-12 fatty acid desaturase (form 3)
    Use
    Tags
    FLAG tag and c-myc tag
    Expression
    Yeast
    Mutation
    Point mutants in FAD2-3 ORF relative to deposited…
    Promoter
    GAL1 and GAL10
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73596-plasmid-map-sequence-id-139406

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  46. pACBE-ASR (pXK-607)

    Plasmid
    #208822
    Purpose
    Universal Antibiotic Screening Reporter for adenine and cytosine base editors. Key Construct: CMV-PuroR*(ATAACG)-eGFP-hU6-gRNA.
    Depositor
    Kun Xu
    Article
    Ma et al J Biol Chem. 2022 Jul;298(7):102103. doi
    Type
    Empty backbone
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, U6
    Available since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208822-plasmid-map-sequence-id-417248

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Showing: 3601 - 3650 of 18941 results