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pKeratin-LSSmOrange

Plasmid

#37138

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Tags

human cytokeratin 18

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pJYDNgp

Plasmid

#162453

Purpose

Positive control plasmid for pJYDNg carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid contains a long 2G linker.

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Insert

Aga2p-2Glinker-eUnaG2 (AGA2 )

Use

Shuttle vector bacteria/yeast

Tags

HA nad myc and eUnaG2 fluorescent protein

Promoter

GAL1

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-Alpha-synuclein-H50Q

Plasmid

#181724

Purpose

Galactose inducible expression of Alpha-synuclein-H50Q

Depositor

Alejandro Chavez

Insert

Alpha-synuclein (SNCA Human)

Expression

Yeast

Mutation

H50Q

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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plvx-AD8

Plasmid

#191558

Purpose

Inducible expression of HIV-1 Env

Depositor

Alon Herschhorn, Joseph Sodroski

Article

Herschhorn et al PLoS One. 2011;6(11):e26731. doi: 10.137

Insert

HIV-1 AD8 env

Use

Lentiviral

Mutation

None

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pX165-Sniper-Cas9

Plasmid

#140560

Purpose

Expresses Sniper Cas9 in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

Cas9

Mutation

F539S M763I K890N

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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APneg-SNAP25d

Plasmid

#168014

Purpose

negative selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

SNAP25-T7PAP

Expression

Bacterial

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pRS306-PTDH3-SUMO10-6SIM

Plasmid

#190248

Purpose

Expression SUMO10-SIM6 in yeast cells

Depositor

Liam Holt

Article

Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

Insert

10SUMO-6SIM

Expression

Yeast

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pet28a-ClbB-NRPS-NHis

Plasmid

#49217

Purpose

NRPS module of ClbB containing CAT domains with N-terminal 6His tag

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbB-NRPS

Tags

6x His tag and T7 tag

Expression

Bacterial

Mutation

contains aa4-1080 of reference sequence NP_754362…

Promoter

T7

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pX165-Cas9-HF1

Plasmid

#140565

Purpose

Expresses Cas9-HF1 in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

Cas9

Mutation

N497A R661A Q695A Q926A

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pReporter_5

Plasmid

#60566

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_5

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pPM2212

Plasmid

#167552

Purpose

Encodes rfb region of Shigella flexneri serotype 2a, which expresses Shigella O antigen in E. coli K-12

Depositor

Renato Morona

Article

Macpherson et al Mol Microbiol. 1991 Jun;5(6):1491-9. doi

Insert

rfb region of Shigella flexneri serotype 2a

Use

Cosmid

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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APneg-SNAP25c

Plasmid

#168013

Purpose

negative selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

SNAP25-T7PAP

Expression

Bacterial

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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APneg-SNAP25b

Plasmid

#168012

Purpose

negative selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

SNAP25-T7PAP

Expression

Bacterial

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCas_5+7_gfp

Plasmid

#60595

Purpose

encodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cas_5+7_gfp

Expression

Bacterial

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCas_2+5

Plasmid

#60594

Purpose

encodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cas_2+5

Expression

Bacterial

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pIntegrase_10

Plasmid

#60581

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_10

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Sam68 G178E

Plasmid

#17689

Depositor

David Shalloway

Article

Lin et al J Biol Chem. 1997 Oct 24. 272(43):27274-

Insert

Sam68 (Khdrbs1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

G178E

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pET28a-R4-uAb

Plasmid

#101801

Purpose

Bacterial cell expression of the beta-galactosidase-specific ubiquibody, R4-uAb

Depositor

Matthew DeLisa

Article

Portnoff et al J Biol Chem. 2014 Mar 14;289(11):7844-55

Insert

R4-uAb

Tags

6xHis and Flag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 22, 2018

Availability

Academic Institutions and Nonprofits only

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psAAVS1-T2

Plasmid

#60543

Purpose

GFP reporter for CRISPR/CAS9 and gRNA_AAVS1-T2 activities

Depositor

William Simonds

Article

Zhang et al J Biotechnol. 2014 Sep 2. pii: S0168-165

Insert

ProtoSpacer AAVS1-T2 (AAVS1 Human)

Use

CRISPR; Kan

Expression

Mammalian

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-Alpha-synuclein-A53T

Plasmid

#181722

Purpose

Galactose inducible expression of Alpha-synuclein-A53T

Depositor

Alejandro Chavez

Insert

Alpha-synuclein (SNCA Human)

Expression

Yeast

Mutation

A53T

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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