We narrowed to 14,435 results for: nts

1. pGEX-6p1-GST-ArcΔCTD

   Plasmid

   #119878

   Purpose

   Bacterial Expression construct for the production and purification of Arc missing C-terminal Domain

   Depositor

   Jason Shepherd

   Article

   Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

   Insert

   Activity Regulated Cytoskeletal Protein (Arc Rat)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Deletion of nt 832-1110

   Promoter

   tac

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. hum alpha ENAC promoter a25-a23

   Plasmid

   #83427

   Purpose

   promoter reporter construct to test 5' flanking sequences

   Depositor

   Christie Thomas

   Article

   Sayegh et al J Biol Chem. 1999 Apr 30;274(18):12431-7

   Insert

   human aENaC gene 5' flanking seq + 55 nt of the 5' UTR for exon 1A (SCNN1A Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Promoter

   alpha ENAC

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-sgNT.3#3/Cre

   Plasmid

   #173662

   Purpose

   Expresses a NT.3-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   Non-targeting gRNA
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

   Plasmid

   #138525

   Purpose

   Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.

   Depositor

   Richard Jenner

   Article

   Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

   Insert

   Control non-PRC2 binding A-tract repeat RNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDF-casBCDE(g8)

   Plasmid

   #89727

   Purpose

   Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing 33-nt spacer (referred to as 'wt') matching a protospacer in the M13 phage genome.

   Depositor

   Konstantin Severinov, Konstantin Kuznedelov

   Article

   Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

   Insert

   CRISPR array
   

   Expression

   Bacterial

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1_SNORD27h_2ntmut

   Plasmid

   #73067

   Purpose

   expression clone for human mutated SNORD27 (C/D box snoRNA U27)

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   SNORD27 (E2F7 Human)
   

   Tags

   no tag

   Expression

   Mammalian

   Mutation

   2 nt mutation in AS box that binds E2F7 pre-mRNA

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pMCh2008_pLenti_Syn_mChe-cdc42E7-E63'UTR

   Plasmid

   #118622

   Purpose

   expression of mChe-tagged cdc42E7-E6-3'UTR under pSyn promoter for primary neurons

   Depositor

   Marina Chekulaeva

   Article

   Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

   Insert

   cdc42E7-E6 3'UTR (Cdc42 Mouse)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   changed ttgcttt to cggtaag (496 - 502 nt in 3…

   Promoter

   synapsin I (rat)

   Available Since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMEXneo-TrkC-Δ6

   Plasmid

   #190204

   Purpose

   To express the human TrkC that has in the extracellular domain only the second IgG like domain. Still binds NT-3.

   Depositor

   Pantelis Tsoulfas

   Article

   Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40.

   Insert

   TrkC (NTRK3 Human)
   

   Expression

   Mammalian

   Mutation

   The extracellualr domain conatins only the signal…

   Promoter

   Moloney mouse sarcoma virus right LTR

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUb FLAG-tws

   Plasmid

   #195068

   Purpose

   Expresses FLAG-tagged Drosophila tws protein

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   twins (tws Fly)
   

   Tags

   3xFLAG

   Expression

   Insect

   Promoter

   Ubi-p63e

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRosettav2

    Plasmid

    #115557

    Purpose

    To determine correct antibiotic selection conditions in mammalian cells; standard for titering experiments

    Depositor

    John Doench

    Insert

    Hygromycin-Blasticidin-Puromycin-EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-ePEG-LINE1-loxPsym

    Plasmid

    #216866

    Purpose

    Lentiviral guide RNA vector to insert the loxPsym sequence into LINE1 elements

    Depositor

    Leopold Parts

    Insert

    spacerLINE1_cr772scaffold_PBS-loxPsm-HA-tevopreQ
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSET.HaloTag.v7.CoOp.Cys-less

    Plasmid

    #195487

    Purpose

    Bacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Insert

    HaloTag.v7
    

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    C61T, C262V

    Promoter

    T7

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDR195

    Plasmid

    #36028

    Depositor

    Dominique Loque, Wolf Frommer

    Article

    Rentsch et al FEBS Lett. 1995 Aug 21;370(3):264-8.

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    pPMA1

    Available Since

    July 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDR196

    Plasmid

    #36029

    Depositor

    Dominique Loque, Doris Rentsch, Wolf Frommer

    Article

    Meyer et al J Biol Chem. 2006 Mar 17;281(11):7197-20

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    June 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGTEVDNIb

    Plasmid

    #122530

    Purpose

    Binary plasmid with a TEV (NIb deleted) clone

    Depositor

    Jose-Antonio Daros

    Article

    Daros et al Methods Mol Biol. 2020;2083:303-312. doi

    Insert

    Tobacco etch virus (NIb deleted) cDNA
    

    Use

    Potyvirus vectors to produce carotenoids in plants

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTV1

    Plasmid

    #122528

    Purpose

    Intermediate plasmid 1 to build tobacco etch virus recombinant clones

    Depositor

    Jose-Antonio Daros

    Article

    Daros et al Methods Mol Biol. 2020;2083:303-312. doi

    Insert

    Tobacco etch virus cDNA fragment
    

    Use

    Potyvirus vectors to produce carotenoids in plants

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTV3

    Plasmid

    #122529

    Purpose

    Intermediate plasmid 3 to build tobacco etch virus recombinant clone

    Depositor

    Jose-Antonio Daros

    Article

    Daros et al Methods Mol Biol. 2020;2083:303-312. doi

    Insert

    Tobacco etch virus cDNA fragment 3
    

    Use

    Potyvirus vectors to produce carotenoids in plants

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPyCAG-Nanog(K140A)-IP

    Plasmid

    #62998

    Purpose

    Expresses Mouse Nanog K140A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

    Depositor

    Shinya Yamanaka

    Insert

    Mouse Nanog K140A Mutant (Nanog Mouse)
    

    Expression

    Mammalian

    Mutation

    Changed Lysine 140 (141 AA in Mouse Protein) to A…

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPyCAG-Nanog(L122A)-IP

    Plasmid

    #62992

    Purpose

    Expresses Mouse Nanog L122A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

    Depositor

    Shinya Yamanaka

    Insert

    Mouse Nanog L122A Mutant (Nanog Mouse)
    

    Expression

    Mammalian

    Mutation

    Changed Leucine 122 (123 AA in Mouse Protein) to …

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPyCAG-Nanog(Y136A)-IP

    Plasmid

    #62995

    Purpose

    Expresses Mouse Nanog Y136A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

    Depositor

    Shinya Yamanaka

    Insert

    Mouse Nanog Y136A Mutant (Nanog Mouse)
    

    Expression

    Mammalian

    Mutation

    Changed Tyrosine 136 (137 AA in Mouse Protein) to…

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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