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Desmin/pAS2-1

Plasmid

#97407

Purpose

Desmin (aa 30 – 103) in DNA-binding domain (BD) vector, used for yeast-two hybrid

Depositor

Rita Shiang

Article

Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

Insert

Desmin (DES Human)

Expression

Yeast

Available Since

Aug. 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pSIN-Arkadia-gRNA2

Plasmid

#180362

Purpose

targeting mouse Arkadia gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Arkadia targeting gRNA (Rnf111 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

human U6

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc-TorT(sp)

Plasmid

#111255

Purpose

Broad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorT signal peptide

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

TorT signal peptide

Expression

Bacterial

Promoter

Pc

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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CMYA5/pACT2

Plasmid

#97208

Purpose

CMYA5 (aa 4039 – 4069) in a GAL4 AD vector, used for yeast-two hybrid

Depositor

Rita Shiang

Article

Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

Insert

CMYA5 (CMYA5 Human)

Expression

Yeast

Available Since

Aug. 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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EC.SurA.pTYB1.a21-281&390-428.L239R

Plasmid

#15959

Depositor

David McKay

Article

Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

Insert

Escherichia coli surA delta PPIase domain 2

Tags

intein

Expression

Bacterial

Mutation

residues 282-389 deleted; L239R mutation

Available Since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pLenti_DualsgRNA_TIMM23_Puro_T2A_BFP2

Plasmid

#236731

Purpose

Dual sgRNA targeting TIMM23 expressing BFP with Puromycin selection

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

TIMM23 (TIMM23 Human)

Use

Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_DualsgRNA_TIMM23_ER-dAPEX-BFP2

Plasmid

#236734

Purpose

Dual sgRNA targeting TIMM23 expressing dAPEX-BFP targeting to ER

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

TIMM23 (TIMM23 Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pRSET-Cm-CspA1

Plasmid

#215398

Purpose

Cm NCPPB382 cspA1 codon-optimized for E. coli recombinant protein expression (6xHis-CspA1)

Depositor

Gitta Coaker

Article

Stevens et al Proc Natl Acad Sci U S A. 2024 Jun 4;121

Insert

cspA1

Tags

6xHis Tag

Expression

Bacterial

Mutation

Codon-optimized for E. coli BL21(DE3)

Promoter

T7 promoter

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-P19-EGFP

Plasmid

#196609

Purpose

siRNA vector expressing TMV P19 and a 720-long EGFP sequence as inverted repeat

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

p19 + EGFP inverted repeat sequence

Tags

6x His tag

Expression

Bacterial

Promoter

AraC

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-P19-NP

Plasmid

#196608

Purpose

siRNA vector expressing TMV P19 and a 425bp-long NP sequence as inverted repeat

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

p19 + Influenza virus NP inverted repeat

Tags

6x His tag

Expression

Bacterial

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK-NP

Plasmid

#196607

Purpose

Dual-Luciferase Reporter vector carrying a 425bp-long Influenza virus NP sequence in forward direction

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

nucleocapsid protein (NP) gene, partial

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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LLP754_L2pV1_NOT_FRT_Rluc_1IF_lacZ

Plasmid

#192399

Purpose

Off state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-Rluc-FRT

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP753_L2pV1_NOT_FRT_Rluc_0I_lacZ

Plasmid

#192398

Purpose

On state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-Rluc-FRT

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP730_L2_pV01_0I_FRT-OCS-TB-rLUC_TCTP-fLUC

Plasmid

#192373

Purpose

To test for the repressive potential of the OCS and Transcription Block (TB) fusion with the FRT sites around it.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-TB-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP733_L2_pV01_0I_FRT-uORF-rLUC_TCTP-fLUC

Plasmid

#192376

Purpose

To test for the repressive potential of the conserved peptide uORF from AT1G36730 with the FRT sites around it.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-uORF-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP727_L2_pV01_0I_TMV-rLUC_TCTP-fLUC

Plasmid

#192370

Purpose

To control for variation introduced to the gene circuits, Rluc expression not affected by the introduction of terminators or recombination target sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP732_L2_pV01_0I_FRT-35Ster-Rluc_lacZ

Plasmid

#192375

Purpose

To test for the repressive potential of the 35S terminator from CaMV with the FRT sites around it.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-35Ster-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP731_L2_pV01_0I_FRT-OCStrunc-Rluc_lacZ

Plasmid

#192374

Purpose

To test for the repressive potential of the OCS variant (truncation) with the FRT sites around it.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCStrunc-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP741_L2pV1_CR_B3RT-Rluc_TCTP-FlpO_lacZ

Plasmid

#192385

Purpose

To test for if Flp is able to de-repress a circuit with B3RT, to rule out cross-reactivity between these recombinases.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP742_L2pV1_CR_FRT-Rluc_TCTP-B3o_lacZ

Plasmid

#192386

Purpose

To test for if B3 is able to de-repress a circuit with FRT, to rule out cross-reactivity between these recombinases.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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