4,180 results

1. pLX313-Firefly luciferase

   Plasmid

   #118017

   Purpose

   Constitutive expression of Firefly luciferase

   Depositor

   William Hahn, David Root

   Article

   Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-TO-dCas9-3XGFP

   Plasmid

   #64107

   Purpose

   Expression of Sp dCas9-3XGFP in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   Sp dCas9
   

   Use

   Lentiviral

   Tags

   3XsfGFP

   Expression

   Mammalian

   Promoter

   CMV-TO

   Available Since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti CMV Puro DEST (w118-1)

   Plasmid

   #17452

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Puro

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBiFC-VN173

   Plasmid

   #22010

   Purpose

   Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

   Depositor

   Chang-Deng Hu

   Article

   Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

   Insert

   VN173
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Venus residus 1-172

   Available Since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBAD-mTagBFP2

   Plasmid

   #34632

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   mTagBFP2
   

   Expression

   Bacterial

   Mutation

   I174A compared to mTagBFP (enhanced photostabilit…

   Available Since

   Jan. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX313-Renilla luciferase

   Plasmid

   #118016

   Purpose

   Constitutive expression of Renilla luciferase

   Depositor

   William Hahn, David Root

   Article

   Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

   Insert

   Renilla luciferase
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3/F-tractin=mStayGold

   Plasmid

   #212019

   Purpose

   For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   F-tractin
   

   Tags

   mStayGold

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. p6XHis-T7(P266L)

   Plasmid

   #174866

   Purpose

   Expresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.

   Depositor

   Anna Pyle

   Article

   Chillon et al Methods Enzymol. 2015;558:3-37. doi: 10.

   Insert

   T7 RNA polymerase
   

   Tags

   6XHis-tag

   Expression

   Bacterial

   Mutation

   P266L

   Promoter

   lac promoter

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. PH-PLCD1-GFP

   Plasmid

   #51407

   Purpose

   Is a biosensor for PI(4,5)P2

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

   Insert

   PH-PLCD1 (aa 1–170) (PLCD1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9(BB)-2A-Hygro

    Plasmid

    #127763

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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