We narrowed to 2,171 results for: D10

1. pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)

   Plasmid

   #79619

   Purpose

   Expresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   3xFlag-PmCDA1

   Expression

   Mammalian

   Mutation

   D10A for nickase Cas9

   Promoter

   pCMV

   Available since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. ANKRD24-TwinStrepII

   Plasmid

   #83190

   Purpose

   Expression of tagged protein in mammalian cells

   Depositor

   Peter Barr-Gillespie

   Article

   Morgan et al Elife. 2016 Aug 15;5:e18312. doi: 10.755

   Insert

   ANKRD24 (Ankrd24 Mouse)
   

   Use

   Tags

   Twin Strep Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDIRECT_21B

   Plasmid

   #91129

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA, Plant Selection: 2x35S:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A, H840A

   Promoter

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT)

   Plasmid

   #79621

   Purpose

   Expresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Mammalian

   Mutation

   D10A and H840A for nuclease deficient Cas9

   Promoter

   pCMV

   Available since

   Sept. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-Beta(4)Integrin-DeltaCYT

   Plasmid

   #16264

   Depositor

   Arthur Mercurio

   Article

   Clarke et al J Biol Chem. 1995 Sep 29. 270(39):22673-

   Insert

   Beta 4 Integrin (ITGB4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   truncated cytoplasmic tail introduced a stop cod…

   Promoter

   Available since

   March 6, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pDIRECT_26G

   Plasmid

   #91149

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:bar

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A, H840A

   Promoter

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SpCas9 TadCBEb

   Plasmid

   #193832

   Purpose

   Express TadCBEb (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-CDb-SpCas9 D10A-2xUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYPQ269C2

   Plasmid

   #164716

   Purpose

   Gateway entry clone (attL1 & attR5) hAID-CBE_V04 for C-T base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

   Insert

   hAID-zCas9(D10A)-UGI-T2A-MS2-UGI
   

   Use

   CRISPR; Gateway compatible haid-cbe_v04 entry clo…

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDIRECT_25G

   Plasmid

   #91144

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A, H840A

   Promoter

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRS0035

    Plasmid

    #131125

    Purpose

    Mammalian Target-AID expression vector

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    nCas9-PmCDA1-UGI
    

    Use

    CRISPR

    Tags

    PmCDA1 and UGI

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CMV promoter

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDIRECT_23D

    Plasmid

    #91141

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A, H840A

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCXLE-dCas9VPP300-T2A-GFP-shP53

    Plasmid

    #102896

    Purpose

    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    dCas9VPP300-T2A-GFP-shP53
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYPQ269A2

    Plasmid

    #164714

    Purpose

    Gateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V04 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    rAPOBEC1-zCas9(D10A)-UGI-T2A-MS2-UGI
    

    Use

    CRISPR; Gateway compatible rapobec1-cbe_v04 entry…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYPQ265

    Plasmid

    #164712

    Purpose

    Gateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    rAPOBEC1-zCas9(D10A)-UGI
    

    Use

    CRISPR; Gateway compatible rapobec1-cbe_v01 entry…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDIRECT_25I

    Plasmid

    #91146

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A, H840A

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SEMA4-bio-His

    Plasmid

    #51827

    Purpose

    Expresses full-length Semaphorin-4D precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SEMA4 (SEMA4D Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYPQ269D

    Plasmid

    #164717

    Purpose

    Gateway entry clone (attL1 & attR5) PmCDA1-CBE_V04 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    zCas9(D10A)-PmCDA1-UGI-T2A-MS2-UGI)
    

    Use

    CRISPR; Gateway compatible pmcda1-cbe_v04 entry c…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJT112_GalL_nCDA1-xBE3

    Plasmid

    #141318

    Purpose

    Expressing base editor nCDA1-xBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    nCDA1-xBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

    Promoter

    GalL

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJMP1341

    Plasmid

    #119272

    Purpose

    control sgRNA in cells lacking rfp

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDIRECT_22D

    Plasmid

    #91136

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A, H840A

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge