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pPRISM-Cre-cmlc2-mRFP

Plasmid

#117793

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR CMVie-Lifeact-iRFP670 L1-R5

Plasmid

#84390

Purpose

Multisite Gateway entry vector for expression of iRFP670-tagged Lifeact in cells including neurons

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

Lifeact

Use

Gateway

Tags

iRFP670

Expression

Mammalian

Mutation

Promoter

CMV immediate early

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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Pdx1-RFP hygro (DM#236)

Plasmid

#15110

Depositor

Douglas Melton

Article

Rajagopal et al Science. 2003 Jan 17. 299(5605):363.

Insert

Pdx1 promoter (Pdx1 Mouse)

Use

Tags

RFP

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pCVL iRFP-T2A-Trex2

Plasmid

#50418

Purpose

Coexpresses Trex2 and iRFP in mammalian cells.

Depositor

Andrew Scharenberg

Inserts

iRFP
Trex2

Use

Lentiviral

Tags

T2A

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pPHR-iRFP713-NLS-VP16

Plasmid

#103823

Purpose

Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-iRFP713-VP16 (CRY2 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

VP16: G126T (silent, G42G)

Promoter

CMV

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-SRGAP2A-FBAR-mRFP

Plasmid

#168502

Purpose

Expression of the F-BAR domain (aa1-501) of SRGAP2 with a C-terminal mRFP fusion.

Depositor

Franck Polleux

Article

Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

Insert

SRGAP2A-FBAR (SRGAP2 Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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PacrAB-rfp + PmutS-yfp

Plasmid

#121442

Purpose

plasmid reporting the expression from the acrAB and mutS promoters

Depositor

Mary Dunlop

Article

El Meouche et al Science. 2018 Nov 9;362(6415):686-690. d

Inserts

PmutS-yfp
PacrAB-rfp

Use

Synthetic Biology; Pbb vector

Tags

Expression

Bacterial

Mutation

Promoter

PacrAB and PmutS

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pExodus iRFP-T2A-Trex2

Plasmid

#50419

Purpose

Coexpresses Trex2 and iRFP in mammalian cells.

Depositor

Andrew Scharenberg

Inserts

iRFP
Trex2

Use

Tags

T2A

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-NLS-tdiRFP670

Plasmid

#82621

Purpose

Expresses NLS-tdiRFP670 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Insert

NLS-tdiRFP670

Use

Piggybac vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-GBP-RFP-hphMX6

Plasmid

#105161

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

Insert

GBP-RFP-hphMX6

Use

Tags

GBP-RFP

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-E1.1-NRSE-RFP

Plasmid

#22929

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

E1.1 binding site from Scardigli et al. 2003, with neuron-restrictive silencing element, with minimal CMV

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pLV.U6gT28.13.EFS-NS.H2B-RFP

Plasmid

#170388

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B-RFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS-NS

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pRFP shRNA-resistant TrkB WT

Plasmid

#72714

Purpose

Mammalian expression of TrkB fused to RFP in C-terminus, shRNA resistant

Depositor

Freddy Jeanneteau

Article

Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

Insert

TrkB

Use

Tags

RFP

Expression

Mammalian

Mutation

shRNA-resistant TrkB (T300C, T301A, C302G, C306A,…

Promoter

CMV

Available since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
pCAG-RFP-miR-E1-1int

Plasmid

#19823

Depositor

Zuoshang Xu

Article

Qiu et al BMC Biotechnol. 2008 . 8():77.

Insert

miRNA

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
pJM105: pTac RFP on pCM66T

Plasmid

#74746

Purpose

pCM66T with promoter pTac driving RFP expression (maxRFP = BBa_E1010)

Depositor

Mary Lidstrom

Insert

RFP (BioBrick part number BBa_E1010)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pTac

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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mRFP-FKBP-INPP5E(D556A)

Plasmid

#183678

Purpose

Recruitable human type IV 5-phosphatase enzyme (catalytically inactive)

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

INPP5E (INPP5E Human)

Use

Tags

mRFP-FKBP12(1-108)

Expression

Mammalian

Mutation

C641A, D556A

Promoter

CMV

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-GBP-RFP-kanMX6

Plasmid

#105160

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

Insert

GBP-RFP-kanMX6

Use

Tags

GBP-RFP

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-DR1-NRSE-RFP

Plasmid

#22927

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

peroxisome proliferator activated receptor gamma binding site promoter with neuron-restrictive silencer element with minimal CMV

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pBS-M71-IRES-tauRFP2ACNf

Plasmid

#15510

Depositor

Peter Mombaerts

Article

Li et al Nature. 2004 Mar 25. 428(6981):393-9.

Insert

M71 targeting vector with IRES tauRFP-RFP ACN

Use

High copy number

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pCMMP-NGFR:LMP1-IRES-mRFP

Plasmid

#36974

Depositor

Bill Sugden

Article

Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 20

Insert

NGFR:LMP1 (NGFR, LMP-1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

NGFR:LMP1 chimeric receptor consists of aa 1–276 …

Promoter

CMV

Available since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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